Neural circuits in the central nervous system are the substrate of various high-order brain functions. Anatomical and functional graph structures of neural networks with actual connections will provide us with perspectives to elucidate the brain complex system. Here, we aim to develop a three-dimensional mouse brain atlas of neural circuits using high resolution x-ray tomography by synchrotron radiation. It will not only identify a large number of synapses but also clarify the structure of neuronal networks for understanding the most complicated organ in the body. In this study, a nano-resolution full-field x-ray microscope revealed nerve fibers and organelles including mitochondria and synapses in the mouse cerebral cortex. Fig. a shows chematic layout of the x-ray optical system and Fig. b demonstrated soft x-ray micrograph of the neural tissue slice. In the near future, we will utilize that information to begin deciphering the wiring diagram of the brain by applying the nano-resolution x-ray tomography.
|Publication status||Published - 15 Nov 2010|
|Event||40th Annual Meeting of the Society for Neuroscience - San Diego, United States|
Duration: 13 Nov 2010 → 17 Nov 2010
Conference number: 40
|Conference||40th Annual Meeting of the Society for Neuroscience|
|Period||13/11/10 → 17/11/10|
Mizutani, H., Sagara, H., Ohigashi, T., & Takagi, T. (2010). X-ray nanoscope with synapse resolution for neuronal circuit reconstruction. Paper presented at 40th Annual Meeting of the Society for Neuroscience, San Diego, United States.