X-ray microscopy for neural circuit reconstruction

Haruo Mizutani, Yoshihiro Takeda, Atsushi Momose, Akihisa Takeuchi, Toshihisa Takagi

Research output: Contribution to journalArticleResearchpeer-review

2 Citations (Scopus)

Abstract

Neural circuits in the central nervous system build our various higher brain functions. However, little is known about mechanisms underlying neuronal information processing in the brain. Anatomical graph structures of real neural networks will provide us with fundamental views to elucidate them. We aim at developing a three-dimensional atlas of neural circuits using high resolution hard X-ray microscopy by synchrotron radiation. We stained neurons of a complete brain from a mouse by the Golgi-Cox method. The heavy metals used in our procedure enhanced X-ray absorption and phase contrast. 3D images of fibriform axons and dendrites of various neurons were reconstructed by back projection. X-ray microscopy with a Talbot interferometer demonstrated finer histological structures of pyramidal neurons in the hippocampus and the cerebral cortex. This observation probably serves as a foundation for achieving a mammalian Connectome Project (identifying complete wiring diagrams of the brain) with X-ray nano-tomography in the near future.

Original languageEnglish
Article number012092
JournalJournal of Physics: Conference Series
Volume186
DOIs
Publication statusPublished - 2009
Externally publishedYes

Fingerprint

brain
neurons
microscopy
x rays
cerebral cortex
hippocampus
axons
central nervous system
wiring
phase contrast
dendrites
heavy metals
mice
synchrotron radiation
interferometers
tomography
projection
fine structure
diagrams
high resolution

Cite this

Mizutani, H., Takeda, Y., Momose, A., Takeuchi, A., & Takagi, T. (2009). X-ray microscopy for neural circuit reconstruction. Journal of Physics: Conference Series, 186, [012092]. https://doi.org/10.1088/1742-6596/186/1/012092
Mizutani, Haruo ; Takeda, Yoshihiro ; Momose, Atsushi ; Takeuchi, Akihisa ; Takagi, Toshihisa. / X-ray microscopy for neural circuit reconstruction. In: Journal of Physics: Conference Series. 2009 ; Vol. 186.
@article{4a6bb56f3bed4953a601c5c5f65e5911,
title = "X-ray microscopy for neural circuit reconstruction",
abstract = "Neural circuits in the central nervous system build our various higher brain functions. However, little is known about mechanisms underlying neuronal information processing in the brain. Anatomical graph structures of real neural networks will provide us with fundamental views to elucidate them. We aim at developing a three-dimensional atlas of neural circuits using high resolution hard X-ray microscopy by synchrotron radiation. We stained neurons of a complete brain from a mouse by the Golgi-Cox method. The heavy metals used in our procedure enhanced X-ray absorption and phase contrast. 3D images of fibriform axons and dendrites of various neurons were reconstructed by back projection. X-ray microscopy with a Talbot interferometer demonstrated finer histological structures of pyramidal neurons in the hippocampus and the cerebral cortex. This observation probably serves as a foundation for achieving a mammalian Connectome Project (identifying complete wiring diagrams of the brain) with X-ray nano-tomography in the near future.",
author = "Haruo Mizutani and Yoshihiro Takeda and Atsushi Momose and Akihisa Takeuchi and Toshihisa Takagi",
year = "2009",
doi = "10.1088/1742-6596/186/1/012092",
language = "English",
volume = "186",
journal = "Journal of Physics: Conference Series",
issn = "1742-6588",
publisher = "IOP Publishing Ltd.",

}

X-ray microscopy for neural circuit reconstruction. / Mizutani, Haruo; Takeda, Yoshihiro; Momose, Atsushi; Takeuchi, Akihisa; Takagi, Toshihisa.

In: Journal of Physics: Conference Series, Vol. 186, 012092, 2009.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - X-ray microscopy for neural circuit reconstruction

AU - Mizutani, Haruo

AU - Takeda, Yoshihiro

AU - Momose, Atsushi

AU - Takeuchi, Akihisa

AU - Takagi, Toshihisa

PY - 2009

Y1 - 2009

N2 - Neural circuits in the central nervous system build our various higher brain functions. However, little is known about mechanisms underlying neuronal information processing in the brain. Anatomical graph structures of real neural networks will provide us with fundamental views to elucidate them. We aim at developing a three-dimensional atlas of neural circuits using high resolution hard X-ray microscopy by synchrotron radiation. We stained neurons of a complete brain from a mouse by the Golgi-Cox method. The heavy metals used in our procedure enhanced X-ray absorption and phase contrast. 3D images of fibriform axons and dendrites of various neurons were reconstructed by back projection. X-ray microscopy with a Talbot interferometer demonstrated finer histological structures of pyramidal neurons in the hippocampus and the cerebral cortex. This observation probably serves as a foundation for achieving a mammalian Connectome Project (identifying complete wiring diagrams of the brain) with X-ray nano-tomography in the near future.

AB - Neural circuits in the central nervous system build our various higher brain functions. However, little is known about mechanisms underlying neuronal information processing in the brain. Anatomical graph structures of real neural networks will provide us with fundamental views to elucidate them. We aim at developing a three-dimensional atlas of neural circuits using high resolution hard X-ray microscopy by synchrotron radiation. We stained neurons of a complete brain from a mouse by the Golgi-Cox method. The heavy metals used in our procedure enhanced X-ray absorption and phase contrast. 3D images of fibriform axons and dendrites of various neurons were reconstructed by back projection. X-ray microscopy with a Talbot interferometer demonstrated finer histological structures of pyramidal neurons in the hippocampus and the cerebral cortex. This observation probably serves as a foundation for achieving a mammalian Connectome Project (identifying complete wiring diagrams of the brain) with X-ray nano-tomography in the near future.

UR - http://www.scopus.com/inward/record.url?scp=73449108045&partnerID=8YFLogxK

U2 - 10.1088/1742-6596/186/1/012092

DO - 10.1088/1742-6596/186/1/012092

M3 - Article

VL - 186

JO - Journal of Physics: Conference Series

JF - Journal of Physics: Conference Series

SN - 1742-6588

M1 - 012092

ER -