The purpose of this study was to verify within- and between-day repeatability and variability in children's oxygen uptake (VO2), gross economy (GE; VO2 divided by speed) and heart rate (HR) during treadmill walking based on self-selected speed (SS). Fourteen children (10.1 ± 1.4 years) undertook three testing sessions over 2 days in which four walking speeds, including SS were tested. Within- and between-day repeatability were assessed using the Bland and Altman method, and coefficients of variability (CV) were determined for each child across exercise bouts and averaged to obtain a mean group CV value for VO2, GE, and HR per speed. Repeated measures analysis of variance showed no statistically significant differences in within- or between-day CV for VO2, GE, or HR at any speed. Repeatability within- and between-day for VO2, GE, and HR for all speeds was verified. These results suggest that submaximal VO2 during treadmill walking is stable and reproducible at a range of speeds based on children's SS.