Manual removal (using conditioner and comb or a wet comb) can be used in the treatment of head lice. Head lice infestation (Pediculosis humanus capitis) is a common problem. It is diagnosed by visualising the lice. As half of people infested with head lice will not scratch, all people in contact with a person affected with head lice should be manually checked for infestations. Wet combing is easily and safely performed at home, but persistence is needed. This article describes the process of head lice removal using a wet comb. It has NHMRC Level 2 evidence of efficacy and no serious adverse effects have been reported. This article forms part of a series on non-drug treatments, which summarise the indications, considerations and the evidence, and where clinicians and patients can find further information.