Variation in H-2 antigen expression in F1 hybrid mice: Analysis using monoclonal antibodies

Helen C O'Neill, R V Blanden

Research output: Contribution to journalArticleResearchpeer-review

14 Citations (Scopus)

Abstract

Cells of (CBA/H X BALB/c)F1 hybrid mice express CBA/H-derived H-2k antigens more weakly than do CBA/H cells, but H-2d antigens are similarly expressed by F1 and BALB/c cells. This was evident when F1 hybrid macrophages were compared with CBA/H and BALB/c macrophages as targets for both alloreactive and H-2 restricted anti-viral TC cells. Quantitative absorption of anti-H-2Kk serum by spleen cells of F1 or CBA/H mice also suggested about 3-fold less H-2Kk antigen on F1 cells. With the use of two anti-H-2Kk monoclonal antibodies, 30R3 and 27R9, the reduced expression of H-2Kk in this F1 hybrid was further analysed in a two-stage radio-immunoassay employing the uptake of 125I-protein A to measure antibody binding. By a thermodynamic approach, estimates were made of the dissociation constant for antibody binding, and of the relative numbers of H-2 molecules expressed by both F1 hybrid and CBA/H spleen cells. The results indicate that there is a two-fold reduction in the number of H-2Kk molecules expressed on the surface of F1 cells. Similar dissociation constants for F1 and CBA/H cells indicated no detectable qualitative difference in their H-2Kk antigens with respect to sites recognized by 30R3 and 27R9.

Original languageEnglish
Pages (from-to)627-635
Number of pages9
JournalAustralian Journal of Experimental Biology and Medical Science
Volume57
Issue number6
DOIs
Publication statusPublished - Dec 1979
Externally publishedYes

Fingerprint

H-2 Antigens
Monoclonal Antibodies
Inbred CBA Mouse
Spleen
Macrophages
Immunologic Techniques
Staphylococcal Protein A
Radio
Thermodynamics
Immunoassay

Cite this

@article{702f1c8ae6284756bf7bdda8cd041f35,
title = "Variation in H-2 antigen expression in F1 hybrid mice: Analysis using monoclonal antibodies",
abstract = "Cells of (CBA/H X BALB/c)F1 hybrid mice express CBA/H-derived H-2k antigens more weakly than do CBA/H cells, but H-2d antigens are similarly expressed by F1 and BALB/c cells. This was evident when F1 hybrid macrophages were compared with CBA/H and BALB/c macrophages as targets for both alloreactive and H-2 restricted anti-viral TC cells. Quantitative absorption of anti-H-2Kk serum by spleen cells of F1 or CBA/H mice also suggested about 3-fold less H-2Kk antigen on F1 cells. With the use of two anti-H-2Kk monoclonal antibodies, 30R3 and 27R9, the reduced expression of H-2Kk in this F1 hybrid was further analysed in a two-stage radio-immunoassay employing the uptake of 125I-protein A to measure antibody binding. By a thermodynamic approach, estimates were made of the dissociation constant for antibody binding, and of the relative numbers of H-2 molecules expressed by both F1 hybrid and CBA/H spleen cells. The results indicate that there is a two-fold reduction in the number of H-2Kk molecules expressed on the surface of F1 cells. Similar dissociation constants for F1 and CBA/H cells indicated no detectable qualitative difference in their H-2Kk antigens with respect to sites recognized by 30R3 and 27R9.",
author = "O'Neill, {Helen C} and Blanden, {R V}",
year = "1979",
month = "12",
doi = "10.1038/icb.1979.66",
language = "English",
volume = "57",
pages = "627--635",
journal = "Australian Journal of Experimental Biology and Medical Science",
issn = "0818-9641",
publisher = "BLACKWELL SCIENCE ASIA",
number = "6",

}

Variation in H-2 antigen expression in F1 hybrid mice : Analysis using monoclonal antibodies. / O'Neill, Helen C; Blanden, R V.

In: Australian Journal of Experimental Biology and Medical Science, Vol. 57, No. 6, 12.1979, p. 627-635.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Variation in H-2 antigen expression in F1 hybrid mice

T2 - Analysis using monoclonal antibodies

AU - O'Neill, Helen C

AU - Blanden, R V

PY - 1979/12

Y1 - 1979/12

N2 - Cells of (CBA/H X BALB/c)F1 hybrid mice express CBA/H-derived H-2k antigens more weakly than do CBA/H cells, but H-2d antigens are similarly expressed by F1 and BALB/c cells. This was evident when F1 hybrid macrophages were compared with CBA/H and BALB/c macrophages as targets for both alloreactive and H-2 restricted anti-viral TC cells. Quantitative absorption of anti-H-2Kk serum by spleen cells of F1 or CBA/H mice also suggested about 3-fold less H-2Kk antigen on F1 cells. With the use of two anti-H-2Kk monoclonal antibodies, 30R3 and 27R9, the reduced expression of H-2Kk in this F1 hybrid was further analysed in a two-stage radio-immunoassay employing the uptake of 125I-protein A to measure antibody binding. By a thermodynamic approach, estimates were made of the dissociation constant for antibody binding, and of the relative numbers of H-2 molecules expressed by both F1 hybrid and CBA/H spleen cells. The results indicate that there is a two-fold reduction in the number of H-2Kk molecules expressed on the surface of F1 cells. Similar dissociation constants for F1 and CBA/H cells indicated no detectable qualitative difference in their H-2Kk antigens with respect to sites recognized by 30R3 and 27R9.

AB - Cells of (CBA/H X BALB/c)F1 hybrid mice express CBA/H-derived H-2k antigens more weakly than do CBA/H cells, but H-2d antigens are similarly expressed by F1 and BALB/c cells. This was evident when F1 hybrid macrophages were compared with CBA/H and BALB/c macrophages as targets for both alloreactive and H-2 restricted anti-viral TC cells. Quantitative absorption of anti-H-2Kk serum by spleen cells of F1 or CBA/H mice also suggested about 3-fold less H-2Kk antigen on F1 cells. With the use of two anti-H-2Kk monoclonal antibodies, 30R3 and 27R9, the reduced expression of H-2Kk in this F1 hybrid was further analysed in a two-stage radio-immunoassay employing the uptake of 125I-protein A to measure antibody binding. By a thermodynamic approach, estimates were made of the dissociation constant for antibody binding, and of the relative numbers of H-2 molecules expressed by both F1 hybrid and CBA/H spleen cells. The results indicate that there is a two-fold reduction in the number of H-2Kk molecules expressed on the surface of F1 cells. Similar dissociation constants for F1 and CBA/H cells indicated no detectable qualitative difference in their H-2Kk antigens with respect to sites recognized by 30R3 and 27R9.

U2 - 10.1038/icb.1979.66

DO - 10.1038/icb.1979.66

M3 - Article

VL - 57

SP - 627

EP - 635

JO - Australian Journal of Experimental Biology and Medical Science

JF - Australian Journal of Experimental Biology and Medical Science

SN - 0818-9641

IS - 6

ER -