TY - JOUR
T1 - Validity and reliability of a novel salivary immunoassay for individual profiling in applied sports science
AU - Coad, Sam
AU - McLellan, Christopher
AU - Whitehouse, Timothy
AU - Gray, Bon
PY - 2015/4/3
Y1 - 2015/4/3
N2 - The aim of this study was to examine the validity and reliability of a novel immunoassay, developed to assess salivary Immunoglobulin A (s-IgA). Validity and reliability of the Individual Profiling Lateral Flow Device (IPRO LFD) for s-IgA concentrations ([s-IgA]) was assessed in males (n = 12) and females (n =13) who were involved in recreational activities. Reliability of the IPRO LFD method was assessed by comparing [s-IgA] of two saliva samples collected concurrently, while validity was assessed by comparing with an enzyme-linked immunosorbent assay (ELISA) method. The IPRO LFD had a strong positive correlation (r = 0.93, p < 0.001), with no difference in [s-IgA] compared with the ELISA. The IPRO LFD was considered reliable (ICC r = 0.89, p < 0.001 and CV = 9.40 %) for measures of [s-IgA]. We concluded that the IPRO LFD method may be a substitute to the ELISA method for measurements of [s-IgA].
AB - The aim of this study was to examine the validity and reliability of a novel immunoassay, developed to assess salivary Immunoglobulin A (s-IgA). Validity and reliability of the Individual Profiling Lateral Flow Device (IPRO LFD) for s-IgA concentrations ([s-IgA]) was assessed in males (n = 12) and females (n =13) who were involved in recreational activities. Reliability of the IPRO LFD method was assessed by comparing [s-IgA] of two saliva samples collected concurrently, while validity was assessed by comparing with an enzyme-linked immunosorbent assay (ELISA) method. The IPRO LFD had a strong positive correlation (r = 0.93, p < 0.001), with no difference in [s-IgA] compared with the ELISA. The IPRO LFD was considered reliable (ICC r = 0.89, p < 0.001 and CV = 9.40 %) for measures of [s-IgA]. We concluded that the IPRO LFD method may be a substitute to the ELISA method for measurements of [s-IgA].
UR - http://www.scopus.com/inward/record.url?scp=84929028207&partnerID=8YFLogxK
U2 - 10.1080/15438627.2015.1005300
DO - 10.1080/15438627.2015.1005300
M3 - Article
C2 - MEDLINE:25639912
AN - SCOPUS:84929028207
SN - 1543-8627
VL - 23
SP - 140
EP - 150
JO - Research in Sports Medicine
JF - Research in Sports Medicine
IS - 2
ER -