Urothelium-derived inhibitory factor(s) influences on detrusor muscle contractility in vitro

M. H. Hawthorn, C. R. Chapple, M. Cock, R. Chess-Williams

Research output: Contribution to journalArticleResearchpeer-review

213 Citations (Scopus)

Abstract

The function of the bladder urothelium in modulating contractile responses of the underlying detrusor smooth muscle to muscarinic stimulation has been examined in the pig bladder. Saturation curves for [ 3H]-QNB binding demonstrated a greater muscarinic receptor density in the urothelium than in the detrusor smooth muscle. The presence of an intact urothelium on isolated bladder strips inhibited contractions induced by carbachol but not KCl. Contractions of a urothelium-denuded muscle strip were inhibited in the presence of a second bladder strip with an intact urothelium, but not if the second strip was denuded. The urothelium-induced inhibition of contractions was not prevented in the presence of L-NOARG, methylene blue, indomethacin, propranolol, suramin, TEA or apamin. The data suggest the presence of a diffusable, urothelium-derived inhibitory factor, which could not be identified but appears to be neither nitric oxide, a cyclo-oxygenase product, a catecholamine, adenosine, GABA nor an EDHF sensitive to apamin.

Original languageEnglish
Pages (from-to)416-419
Number of pages4
JournalBritish Journal of Pharmacology
Volume129
Issue number3
DOIs
Publication statusPublished - 2000
Externally publishedYes

Fingerprint

Urothelium
Muscles
Urinary Bladder
Apamin
Smooth Muscle
Suramin
Nitroarginine
Methylene Blue
Carbachol
Muscarinic Receptors
Prostaglandin-Endoperoxide Synthases
In Vitro Techniques
Propranolol
Indomethacin
Adenosine
gamma-Aminobutyric Acid
Cholinergic Agents
Catecholamines
Nitric Oxide
Swine

Cite this

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title = "Urothelium-derived inhibitory factor(s) influences on detrusor muscle contractility in vitro",
abstract = "The function of the bladder urothelium in modulating contractile responses of the underlying detrusor smooth muscle to muscarinic stimulation has been examined in the pig bladder. Saturation curves for [ 3H]-QNB binding demonstrated a greater muscarinic receptor density in the urothelium than in the detrusor smooth muscle. The presence of an intact urothelium on isolated bladder strips inhibited contractions induced by carbachol but not KCl. Contractions of a urothelium-denuded muscle strip were inhibited in the presence of a second bladder strip with an intact urothelium, but not if the second strip was denuded. The urothelium-induced inhibition of contractions was not prevented in the presence of L-NOARG, methylene blue, indomethacin, propranolol, suramin, TEA or apamin. The data suggest the presence of a diffusable, urothelium-derived inhibitory factor, which could not be identified but appears to be neither nitric oxide, a cyclo-oxygenase product, a catecholamine, adenosine, GABA nor an EDHF sensitive to apamin.",
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journal = "British journal of pharmacology and chemotherapy",
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Urothelium-derived inhibitory factor(s) influences on detrusor muscle contractility in vitro. / Hawthorn, M. H.; Chapple, C. R.; Cock, M.; Chess-Williams, R.

In: British Journal of Pharmacology, Vol. 129, No. 3, 2000, p. 416-419.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Cock, M.

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