Urothelium derived inhibitory factor and cross-talk among receptors in the trigone of the bladder of the pig

L. Templeman, C. R. Chapple, R. Chess-Williams

Research output: Contribution to journalArticleResearchpeer-review

60 Citations (Scopus)

Abstract

Purpose: In the dome of the bladder of the pig muscarinic receptor stimulation has been shown to release a factor from the urothelium that exerts an inhibitory effect on the underlying smooth muscle. We examined whether the urothelium in the trigone of the bladder also releases this factor, identified which receptors stimulate its release and investigated possible cross-talk among these receptor systems in the trigone. Materials and Methods: Paired longitudinal strips of pig bladder were isolated, the urothelium was removed from 1 strip per pair and tissues were set up in gassed Krebs solution at 37C. Cumulative concentration-response curves to carbachol, phenylephrine or histamine were constructed. In some tissues a second phenylephrine curve was constructed in the presence of 1 μM. carbachol or 1 μM. histamine. In a further group of tissues the second phenylephrine curve was constructed in the presence of 1 μM. carbachol and 1 μM. atropine or 1 μM. histamine and 100 nM. mepyramine. Results: In the presence of an intact urothelium contractile responses to carbachol and histamine but not to phenylephrine were depressed. In the presence of 1 μM. carbachol or 1 μM. histamine the responses of intact urothelium strips to phenylephrine were significantly depressed. This effect was absent in the presence of atropine and mepyramine, respectively. Conclusions: Carbachol and histamine induce the release of a urothelium derived inhibitory factor in the bladder trigone. The factor appears to mediate cross-talk between these systems and the α-adrenoceptor system in this region of the bladder.

Original languageEnglish
Pages (from-to)742-745
Number of pages4
JournalJournal of Urology
Volume167
Issue number2 I
DOIs
Publication statusPublished - 2002
Externally publishedYes

Fingerprint

Receptor Cross-Talk
Urothelium
Carbachol
Phenylephrine
Urinary Bladder
Swine
Histamine
Pyrilamine
Atropine
Histamine Release
Muscarinic Receptors
Adrenergic Receptors
Smooth Muscle

Cite this

@article{038f458b9c6241bd8c0bc2991f1cba66,
title = "Urothelium derived inhibitory factor and cross-talk among receptors in the trigone of the bladder of the pig",
abstract = "Purpose: In the dome of the bladder of the pig muscarinic receptor stimulation has been shown to release a factor from the urothelium that exerts an inhibitory effect on the underlying smooth muscle. We examined whether the urothelium in the trigone of the bladder also releases this factor, identified which receptors stimulate its release and investigated possible cross-talk among these receptor systems in the trigone. Materials and Methods: Paired longitudinal strips of pig bladder were isolated, the urothelium was removed from 1 strip per pair and tissues were set up in gassed Krebs solution at 37C. Cumulative concentration-response curves to carbachol, phenylephrine or histamine were constructed. In some tissues a second phenylephrine curve was constructed in the presence of 1 μM. carbachol or 1 μM. histamine. In a further group of tissues the second phenylephrine curve was constructed in the presence of 1 μM. carbachol and 1 μM. atropine or 1 μM. histamine and 100 nM. mepyramine. Results: In the presence of an intact urothelium contractile responses to carbachol and histamine but not to phenylephrine were depressed. In the presence of 1 μM. carbachol or 1 μM. histamine the responses of intact urothelium strips to phenylephrine were significantly depressed. This effect was absent in the presence of atropine and mepyramine, respectively. Conclusions: Carbachol and histamine induce the release of a urothelium derived inhibitory factor in the bladder trigone. The factor appears to mediate cross-talk between these systems and the α-adrenoceptor system in this region of the bladder.",
author = "L. Templeman and Chapple, {C. R.} and R. Chess-Williams",
year = "2002",
doi = "10.1016/S0022-5347(01)69137-7",
language = "English",
volume = "167",
pages = "742--745",
journal = "Journal of Urology",
issn = "0022-5347",
publisher = "Elsevier",
number = "2 I",

}

Urothelium derived inhibitory factor and cross-talk among receptors in the trigone of the bladder of the pig. / Templeman, L.; Chapple, C. R.; Chess-Williams, R.

In: Journal of Urology, Vol. 167, No. 2 I, 2002, p. 742-745.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Urothelium derived inhibitory factor and cross-talk among receptors in the trigone of the bladder of the pig

AU - Templeman, L.

AU - Chapple, C. R.

AU - Chess-Williams, R.

PY - 2002

Y1 - 2002

N2 - Purpose: In the dome of the bladder of the pig muscarinic receptor stimulation has been shown to release a factor from the urothelium that exerts an inhibitory effect on the underlying smooth muscle. We examined whether the urothelium in the trigone of the bladder also releases this factor, identified which receptors stimulate its release and investigated possible cross-talk among these receptor systems in the trigone. Materials and Methods: Paired longitudinal strips of pig bladder were isolated, the urothelium was removed from 1 strip per pair and tissues were set up in gassed Krebs solution at 37C. Cumulative concentration-response curves to carbachol, phenylephrine or histamine were constructed. In some tissues a second phenylephrine curve was constructed in the presence of 1 μM. carbachol or 1 μM. histamine. In a further group of tissues the second phenylephrine curve was constructed in the presence of 1 μM. carbachol and 1 μM. atropine or 1 μM. histamine and 100 nM. mepyramine. Results: In the presence of an intact urothelium contractile responses to carbachol and histamine but not to phenylephrine were depressed. In the presence of 1 μM. carbachol or 1 μM. histamine the responses of intact urothelium strips to phenylephrine were significantly depressed. This effect was absent in the presence of atropine and mepyramine, respectively. Conclusions: Carbachol and histamine induce the release of a urothelium derived inhibitory factor in the bladder trigone. The factor appears to mediate cross-talk between these systems and the α-adrenoceptor system in this region of the bladder.

AB - Purpose: In the dome of the bladder of the pig muscarinic receptor stimulation has been shown to release a factor from the urothelium that exerts an inhibitory effect on the underlying smooth muscle. We examined whether the urothelium in the trigone of the bladder also releases this factor, identified which receptors stimulate its release and investigated possible cross-talk among these receptor systems in the trigone. Materials and Methods: Paired longitudinal strips of pig bladder were isolated, the urothelium was removed from 1 strip per pair and tissues were set up in gassed Krebs solution at 37C. Cumulative concentration-response curves to carbachol, phenylephrine or histamine were constructed. In some tissues a second phenylephrine curve was constructed in the presence of 1 μM. carbachol or 1 μM. histamine. In a further group of tissues the second phenylephrine curve was constructed in the presence of 1 μM. carbachol and 1 μM. atropine or 1 μM. histamine and 100 nM. mepyramine. Results: In the presence of an intact urothelium contractile responses to carbachol and histamine but not to phenylephrine were depressed. In the presence of 1 μM. carbachol or 1 μM. histamine the responses of intact urothelium strips to phenylephrine were significantly depressed. This effect was absent in the presence of atropine and mepyramine, respectively. Conclusions: Carbachol and histamine induce the release of a urothelium derived inhibitory factor in the bladder trigone. The factor appears to mediate cross-talk between these systems and the α-adrenoceptor system in this region of the bladder.

UR - http://www.scopus.com/inward/record.url?scp=0036144876&partnerID=8YFLogxK

U2 - 10.1016/S0022-5347(01)69137-7

DO - 10.1016/S0022-5347(01)69137-7

M3 - Article

VL - 167

SP - 742

EP - 745

JO - Journal of Urology

JF - Journal of Urology

SN - 0022-5347

IS - 2 I

ER -