Spleen tissue regeneration from neonatal murine spleen capsule has previously been demonstrated by our group. In this model, spleen from 3 day-old mice were dissociated to isolate spleen capsule that contains stromal cells before transplantation under the kidney capsule of adult recipient mice. Tissue regenerated after 4 weeks displayed all structural features typical of spleen and functional capacity in terms of antibody class switching and affinity maturation. We have now used the same procedure to investigate the role of host lymphoid inducer cell (LTi) and lymphotoxin expression in tissue development from spleen capsule grafts. Furthermore, we have developed a technique for transplantation of spleen cell aggregates that we are using to identify stromal populations responsible for spleen regeneration.
|Publication status||Published - 18 May 2012|
|Event||The 3rd Workshop of Synthetic Immunology - Kyoto University, Kyoto, Japan|
Duration: 18 May 2012 → 19 May 2012
|Workshop||The 3rd Workshop of Synthetic Immunology|
|Period||18/05/12 → 19/05/12|