Understanding regulation of spleen tissue regeneration

Jonathan Kah Huat Tan, Takeshi Watanabe

Research output: Contribution to conferenceAbstractResearch

Abstract

Spleen tissue regeneration from neonatal murine spleen capsule has previously been demonstrated by our group. In this model, spleen from 3 day-old mice were dissociated to isolate spleen capsule that contains stromal cells before transplantation under the kidney capsule of adult recipient mice. Tissue regenerated after 4 weeks displayed all structural features typical of spleen and functional capacity in terms of antibody class switching and affinity maturation. We have now used the same procedure to investigate the role of host lymphoid inducer cell (LTi) and lymphotoxin expression in tissue development from spleen capsule grafts. Furthermore, we have developed a technique for transplantation of spleen cell aggregates that we are using to identify stromal populations responsible for spleen regeneration.
Original languageEnglish
Publication statusPublished - 18 May 2012
Externally publishedYes
EventThe 3rd Workshop of Synthetic Immunology - Kyoto University, Kyoto, Japan
Duration: 18 May 201219 May 2012

Workshop

WorkshopThe 3rd Workshop of Synthetic Immunology
CountryJapan
CityKyoto
Period18/05/1219/05/12

Fingerprint

Regeneration
Spleen
Capsules
Cell Transplantation
Immunoglobulin Class Switching
Lymphotoxin-alpha
Antibody Affinity
Immunoglobulin Isotypes
Stromal Cells
Helper-Inducer T-Lymphocytes
Lymphocytes
Transplants
Kidney
Population

Cite this

Tan, J. K. H., & Watanabe, T. (2012). Understanding regulation of spleen tissue regeneration. Abstract from The 3rd Workshop of Synthetic Immunology, Kyoto, Japan.
Tan, Jonathan Kah Huat ; Watanabe, Takeshi. / Understanding regulation of spleen tissue regeneration. Abstract from The 3rd Workshop of Synthetic Immunology, Kyoto, Japan.
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abstract = "Spleen tissue regeneration from neonatal murine spleen capsule has previously been demonstrated by our group. In this model, spleen from 3 day-old mice were dissociated to isolate spleen capsule that contains stromal cells before transplantation under the kidney capsule of adult recipient mice. Tissue regenerated after 4 weeks displayed all structural features typical of spleen and functional capacity in terms of antibody class switching and affinity maturation. We have now used the same procedure to investigate the role of host lymphoid inducer cell (LTi) and lymphotoxin expression in tissue development from spleen capsule grafts. Furthermore, we have developed a technique for transplantation of spleen cell aggregates that we are using to identify stromal populations responsible for spleen regeneration.",
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Tan, JKH & Watanabe, T 2012, 'Understanding regulation of spleen tissue regeneration' The 3rd Workshop of Synthetic Immunology, Kyoto, Japan, 18/05/12 - 19/05/12, .

Understanding regulation of spleen tissue regeneration. / Tan, Jonathan Kah Huat; Watanabe, Takeshi.

2012. Abstract from The 3rd Workshop of Synthetic Immunology, Kyoto, Japan.

Research output: Contribution to conferenceAbstractResearch

TY - CONF

T1 - Understanding regulation of spleen tissue regeneration

AU - Tan, Jonathan Kah Huat

AU - Watanabe, Takeshi

PY - 2012/5/18

Y1 - 2012/5/18

N2 - Spleen tissue regeneration from neonatal murine spleen capsule has previously been demonstrated by our group. In this model, spleen from 3 day-old mice were dissociated to isolate spleen capsule that contains stromal cells before transplantation under the kidney capsule of adult recipient mice. Tissue regenerated after 4 weeks displayed all structural features typical of spleen and functional capacity in terms of antibody class switching and affinity maturation. We have now used the same procedure to investigate the role of host lymphoid inducer cell (LTi) and lymphotoxin expression in tissue development from spleen capsule grafts. Furthermore, we have developed a technique for transplantation of spleen cell aggregates that we are using to identify stromal populations responsible for spleen regeneration.

AB - Spleen tissue regeneration from neonatal murine spleen capsule has previously been demonstrated by our group. In this model, spleen from 3 day-old mice were dissociated to isolate spleen capsule that contains stromal cells before transplantation under the kidney capsule of adult recipient mice. Tissue regenerated after 4 weeks displayed all structural features typical of spleen and functional capacity in terms of antibody class switching and affinity maturation. We have now used the same procedure to investigate the role of host lymphoid inducer cell (LTi) and lymphotoxin expression in tissue development from spleen capsule grafts. Furthermore, we have developed a technique for transplantation of spleen cell aggregates that we are using to identify stromal populations responsible for spleen regeneration.

M3 - Abstract

ER -

Tan JKH, Watanabe T. Understanding regulation of spleen tissue regeneration. 2012. Abstract from The 3rd Workshop of Synthetic Immunology, Kyoto, Japan.