Two phases of replacement replenish the olfactory ensheathing cell population after injury in postnatal mice

Fatemeh Chehrehasa, Jenny A K Ekberg, Katie E. Lineburg, Daniel A. Amaya, Alan Mackay-Sim, James A. St John

Research output: Contribution to journalArticleResearchpeer-review

17 Citations (Scopus)

Abstract

Olfactory ensheathing cells (OECs) support the regeneration of olfactory sensory neurons throughout life, however, it remains unclear how OECs respond to a major injury. We have examined the proliferation and migration of OECs following unilateral bulbectomy in postnatal mice. S100ß-DsRed and OMP-ZsGreen transgenic mice were used to visualize OECs and olfactory neurons, respectively, and we used the thymidine analogue ethynyl deoxyuridine (EdU) to identify cells that were proliferating at the time of administration. Following unilateral bulbectomy, there was an initial phase of OEC proliferation throughout the olfactory pathway with a peak of proliferation occurring 2 to 7 days after the injury. A second phase of proliferation also occurred in which precursors localized within the olfactory mucosa divided to replenish the OEC population. We then tracked the positions of OECs that had proliferated and found that there was a progressive increase in OECs in the cavity for at least 12 to 16 days after injury which could not be accounted for solely by local proliferation of OECs within the cavity. These results suggest that OECs migrated from the peripheral olfactory nerve to populate the mass of cells that filled cavity left by bulbectomy. Our results demonstrate that following injury to the olfactory nervous system, the OEC population is replenished by migration of cells that arise from both local proliferation of OECs throughout the olfactory nerve pathway as well as from precursor cells in the olfactory mucosa.

Original languageEnglish
Pages (from-to)322-332
Number of pages11
JournalGlia
Volume60
Issue number2
DOIs
Publication statusPublished - Feb 2012
Externally publishedYes

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Wounds and Injuries
Population
Olfactory Nerve
Olfactory Mucosa
Olfactory Pathways
Olfactory Receptor Neurons
Deoxyuridine
Peripheral Nerves
Thymidine
Nervous System
Transgenic Mice
Cell Movement
Regeneration
Cell Proliferation
Neurons

Cite this

Chehrehasa, F., Ekberg, J. A. K., Lineburg, K. E., Amaya, D. A., Mackay-Sim, A., & St John, J. A. (2012). Two phases of replacement replenish the olfactory ensheathing cell population after injury in postnatal mice. Glia, 60(2), 322-332. https://doi.org/10.1002/glia.22267
Chehrehasa, Fatemeh ; Ekberg, Jenny A K ; Lineburg, Katie E. ; Amaya, Daniel A. ; Mackay-Sim, Alan ; St John, James A. / Two phases of replacement replenish the olfactory ensheathing cell population after injury in postnatal mice. In: Glia. 2012 ; Vol. 60, No. 2. pp. 322-332.
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Chehrehasa, F, Ekberg, JAK, Lineburg, KE, Amaya, DA, Mackay-Sim, A & St John, JA 2012, 'Two phases of replacement replenish the olfactory ensheathing cell population after injury in postnatal mice' Glia, vol. 60, no. 2, pp. 322-332. https://doi.org/10.1002/glia.22267

Two phases of replacement replenish the olfactory ensheathing cell population after injury in postnatal mice. / Chehrehasa, Fatemeh; Ekberg, Jenny A K; Lineburg, Katie E.; Amaya, Daniel A.; Mackay-Sim, Alan; St John, James A.

In: Glia, Vol. 60, No. 2, 02.2012, p. 322-332.

Research output: Contribution to journalArticleResearchpeer-review

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Chehrehasa F, Ekberg JAK, Lineburg KE, Amaya DA, Mackay-Sim A, St John JA. Two phases of replacement replenish the olfactory ensheathing cell population after injury in postnatal mice. Glia. 2012 Feb;60(2):322-332. https://doi.org/10.1002/glia.22267