Towards the Generation of Induced Pluripotent Stem Cells from Non-Eutherian Mammals

Ioannis Jason Limnios, Klaus Matthaei, Margaret Delbridge, Jennifer A M Graves, Ernst Wolvetang

Research output: Contribution to conferencePosterResearchpeer-review

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Abstract

In vitro models of marsupial and monotreme embryogenesis wouldprovide powerful platforms to study mammalian pluripotency andevolution. Despite several efforts, no marsupial embryonic stem(ES) cells have been established, and to our knowledge noattempts have been made to isolate monotreme ES cells. Advancesin cell reprogramming offer functional alternatives to obtainingpluripotent stem cell lines: the induction of pluripotency in somaticcells1 & 2. We are investigating methods to generate and cultureInduced Pluripotent Stem (iPS) cells from marsupials andmonotremes.This project faces two major challenges:1) Generating iPS cells in species for which ES cells have not beenpreviously isolated.2) Identifying species-specific culture conditions and methods thatmaintain pluripotency in vitro.We have begun studying the effects of over-expressing the humanreprogramming factors (Oct4, Sox2, Nanog, LIN28, Klf4 and cMyc)in human, marsupial and monotreme fibroblasts, cultured undervaried culture conditions.Here we present preliminary data from reprogramming experimentswhich suggest partial reprogramming of marsupial and monotremefibroblasts and highlights the need to empirically determine cultureconditions that support the long-term maintenance of pluripotencyin vitro.
Original languageEnglish
Publication statusPublished - 7 Nov 2009
Externally publishedYes
EventThe 56th Annual conference of the Genetics Society of Australasia - University of Queensland, Brisbane, Australia
Duration: 7 Nov 20099 Nov 2009
Conference number: 56th

Conference

ConferenceThe 56th Annual conference of the Genetics Society of Australasia
Abbreviated titleGSA2009
CountryAustralia
CityBrisbane
Period7/11/099/11/09

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Marsupialia
Induced Pluripotent Stem Cells
Mammals
Embryonic Stem Cells
Pluripotent Stem Cells
Embryonic Development
Stem Cells
Fibroblasts
Cell Line

Cite this

Limnios, I. J., Matthaei, K., Delbridge, M., Graves, J. A. M., & Wolvetang, E. (2009). Towards the Generation of Induced Pluripotent Stem Cells from Non-Eutherian Mammals. Poster session presented at The 56th Annual conference of the Genetics Society of Australasia, Brisbane, Australia.
Limnios, Ioannis Jason ; Matthaei, Klaus ; Delbridge, Margaret ; Graves, Jennifer A M ; Wolvetang, Ernst. / Towards the Generation of Induced Pluripotent Stem Cells from Non-Eutherian Mammals. Poster session presented at The 56th Annual conference of the Genetics Society of Australasia, Brisbane, Australia.
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Limnios, IJ, Matthaei, K, Delbridge, M, Graves, JAM & Wolvetang, E 2009, 'Towards the Generation of Induced Pluripotent Stem Cells from Non-Eutherian Mammals' The 56th Annual conference of the Genetics Society of Australasia, Brisbane, Australia, 7/11/09 - 9/11/09, .

Towards the Generation of Induced Pluripotent Stem Cells from Non-Eutherian Mammals. / Limnios, Ioannis Jason; Matthaei, Klaus; Delbridge, Margaret; Graves, Jennifer A M; Wolvetang, Ernst.

2009. Poster session presented at The 56th Annual conference of the Genetics Society of Australasia, Brisbane, Australia.

Research output: Contribution to conferencePosterResearchpeer-review

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T1 - Towards the Generation of Induced Pluripotent Stem Cells from Non-Eutherian Mammals

AU - Limnios, Ioannis Jason

AU - Matthaei, Klaus

AU - Delbridge, Margaret

AU - Graves, Jennifer A M

AU - Wolvetang, Ernst

PY - 2009/11/7

Y1 - 2009/11/7

N2 - In vitro models of marsupial and monotreme embryogenesis wouldprovide powerful platforms to study mammalian pluripotency andevolution. Despite several efforts, no marsupial embryonic stem(ES) cells have been established, and to our knowledge noattempts have been made to isolate monotreme ES cells. Advancesin cell reprogramming offer functional alternatives to obtainingpluripotent stem cell lines: the induction of pluripotency in somaticcells1 & 2. We are investigating methods to generate and cultureInduced Pluripotent Stem (iPS) cells from marsupials andmonotremes.This project faces two major challenges:1) Generating iPS cells in species for which ES cells have not beenpreviously isolated.2) Identifying species-specific culture conditions and methods thatmaintain pluripotency in vitro.We have begun studying the effects of over-expressing the humanreprogramming factors (Oct4, Sox2, Nanog, LIN28, Klf4 and cMyc)in human, marsupial and monotreme fibroblasts, cultured undervaried culture conditions.Here we present preliminary data from reprogramming experimentswhich suggest partial reprogramming of marsupial and monotremefibroblasts and highlights the need to empirically determine cultureconditions that support the long-term maintenance of pluripotencyin vitro.

AB - In vitro models of marsupial and monotreme embryogenesis wouldprovide powerful platforms to study mammalian pluripotency andevolution. Despite several efforts, no marsupial embryonic stem(ES) cells have been established, and to our knowledge noattempts have been made to isolate monotreme ES cells. Advancesin cell reprogramming offer functional alternatives to obtainingpluripotent stem cell lines: the induction of pluripotency in somaticcells1 & 2. We are investigating methods to generate and cultureInduced Pluripotent Stem (iPS) cells from marsupials andmonotremes.This project faces two major challenges:1) Generating iPS cells in species for which ES cells have not beenpreviously isolated.2) Identifying species-specific culture conditions and methods thatmaintain pluripotency in vitro.We have begun studying the effects of over-expressing the humanreprogramming factors (Oct4, Sox2, Nanog, LIN28, Klf4 and cMyc)in human, marsupial and monotreme fibroblasts, cultured undervaried culture conditions.Here we present preliminary data from reprogramming experimentswhich suggest partial reprogramming of marsupial and monotremefibroblasts and highlights the need to empirically determine cultureconditions that support the long-term maintenance of pluripotencyin vitro.

M3 - Poster

ER -

Limnios IJ, Matthaei K, Delbridge M, Graves JAM, Wolvetang E. Towards the Generation of Induced Pluripotent Stem Cells from Non-Eutherian Mammals. 2009. Poster session presented at The 56th Annual conference of the Genetics Society of Australasia, Brisbane, Australia.