The surface of articular cartilage contains a progenitor cell population

GP Dowthwaite, Joanna Bishop, SN Redman, P Rooney, D J R Evans, L Haughton, Z Bayram, B Thomson, C. W. Archer, M Wolfe, S Boyer

Research output: Contribution to journalArticleResearchpeer-review

Abstract

It is becoming increasingly apparent that articular cartilage growth is achieved by apposition from the articular surface. For such a mechanism to occur, a population of stem/progenitor cells must reside within the articular cartilage to provide transit amplifying progeny for growth. Here, we report on the isolation of an articular cartilage progenitor cell from the surface zone of articular cartilage using differential adhesion to fibronectin. This population of cells exhibits high affinity for fibronectin, possesses a high colony-forming efficiency and expresses the cell fate selector gene Notch 1. Inhibition of Notch signalling abolishes colony forming ability whilst activated Notch rescues this inhibition. The progenitor population also exhibits phenotypic plasticity in its differentiation pathway in an embryonic chick tracking system, such that chondroprogenitors can engraft into a variety of connective tissue types including bone, tendon and perimysium. The identification of a chondrocyte subpopulation with progenitor-like characteristics will allow for advances in our understanding of both cartilage growth and maintenance as well as provide novel solutions to articular cartilage repair.
Original languageEnglish
Article number117
Pages (from-to)889-897
JournalJournal of Cell Science
Volume117
Issue number6
DOIs
Publication statusPublished - 2004
Externally publishedYes

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Articular Cartilage
Stem Cells
Population
Fibronectins
Growth
Chondrocytes
Tendons
Connective Tissue
Cartilage
Joints
Maintenance
Bone and Bones
Genes

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Dowthwaite, GP., Bishop, J., Redman, SN., Rooney, P., Evans, D. J. R., Haughton, L., ... Boyer , S. (2004). The surface of articular cartilage contains a progenitor cell population. Journal of Cell Science , 117(6), 889-897. [117]. https://doi.org/10.1242/jcs.00912
Dowthwaite, GP ; Bishop, Joanna ; Redman, SN ; Rooney, P ; Evans, D J R ; Haughton, L ; Bayram, Z ; Thomson, B ; Archer, C. W. ; Wolfe, M ; Boyer , S. / The surface of articular cartilage contains a progenitor cell population. In: Journal of Cell Science . 2004 ; Vol. 117, No. 6. pp. 889-897.
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abstract = "It is becoming increasingly apparent that articular cartilage growth is achieved by apposition from the articular surface. For such a mechanism to occur, a population of stem/progenitor cells must reside within the articular cartilage to provide transit amplifying progeny for growth. Here, we report on the isolation of an articular cartilage progenitor cell from the surface zone of articular cartilage using differential adhesion to fibronectin. This population of cells exhibits high affinity for fibronectin, possesses a high colony-forming efficiency and expresses the cell fate selector gene Notch 1. Inhibition of Notch signalling abolishes colony forming ability whilst activated Notch rescues this inhibition. The progenitor population also exhibits phenotypic plasticity in its differentiation pathway in an embryonic chick tracking system, such that chondroprogenitors can engraft into a variety of connective tissue types including bone, tendon and perimysium. The identification of a chondrocyte subpopulation with progenitor-like characteristics will allow for advances in our understanding of both cartilage growth and maintenance as well as provide novel solutions to articular cartilage repair.",
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Dowthwaite, GP, Bishop, J, Redman, SN, Rooney, P, Evans, DJR, Haughton, L, Bayram, Z, Thomson, B, Archer, CW, Wolfe, M & Boyer , S 2004, 'The surface of articular cartilage contains a progenitor cell population' Journal of Cell Science , vol. 117, no. 6, 117, pp. 889-897. https://doi.org/10.1242/jcs.00912

The surface of articular cartilage contains a progenitor cell population. / Dowthwaite, GP; Bishop, Joanna ; Redman, SN; Rooney, P; Evans, D J R ; Haughton, L; Bayram, Z; Thomson, B; Archer, C. W.; Wolfe, M; Boyer , S.

In: Journal of Cell Science , Vol. 117, No. 6, 117, 2004, p. 889-897.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Dowthwaite, GP

AU - Bishop, Joanna

AU - Redman, SN

AU - Rooney, P

AU - Evans, D J R

AU - Haughton, L

AU - Bayram, Z

AU - Thomson, B

AU - Archer, C. W.

AU - Wolfe, M

AU - Boyer , S

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AB - It is becoming increasingly apparent that articular cartilage growth is achieved by apposition from the articular surface. For such a mechanism to occur, a population of stem/progenitor cells must reside within the articular cartilage to provide transit amplifying progeny for growth. Here, we report on the isolation of an articular cartilage progenitor cell from the surface zone of articular cartilage using differential adhesion to fibronectin. This population of cells exhibits high affinity for fibronectin, possesses a high colony-forming efficiency and expresses the cell fate selector gene Notch 1. Inhibition of Notch signalling abolishes colony forming ability whilst activated Notch rescues this inhibition. The progenitor population also exhibits phenotypic plasticity in its differentiation pathway in an embryonic chick tracking system, such that chondroprogenitors can engraft into a variety of connective tissue types including bone, tendon and perimysium. The identification of a chondrocyte subpopulation with progenitor-like characteristics will allow for advances in our understanding of both cartilage growth and maintenance as well as provide novel solutions to articular cartilage repair.

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Dowthwaite GP, Bishop J, Redman SN, Rooney P, Evans DJR, Haughton L et al. The surface of articular cartilage contains a progenitor cell population. Journal of Cell Science . 2004;117(6):889-897. 117. https://doi.org/10.1242/jcs.00912