The presence of serotonin (5-HT1) receptors negatively coupled to adenylate cyclase in rabbit and human iris-ciliary processes

Nigel L. Barnett, Neville N. Osborne

Research output: Contribution to journalArticleResearchpeer-review

47 Citations (Scopus)

Abstract

Serotonin (5-hydroxytryptamine, 5-HT) reduces forskolin-induced stimulation of cyclic AMP in rabbit iris-ciliary body (ICB) homogenates. The effect is dose dependent and can be mimicked by a number of 5-HT1 receptor agonists including 5-carboxamidotryptamine (5-CT) and RU 24969 [5-methoxy-3-(1,2,3,6-tetrahydro-4-pyridinyl)-1-indole]. The inhibitory effects 5-CT and the 5-HT(1A) selective agent 8-hydroxy-2-(di-n-propyl-amino) tetralin (8-OH-DPAT) on forskolin stimulated adenylate cyclase activity are greater in isolated ciliary processes than in the iris musculature. Spiperone and propranolol significantly antagonize the action of 5-CT in the iris-ciliary body, while ketanserin (5-HT2 antagonist) and ICS 205930 (5-HT(3/4 blocker) were without influence, indicating the presence of the 5-HT(1A) subtype of receptor. Studies carried out on human ICB homogenates also suggest the presence of 5-HT(1A)-like receptors, although these receptors are not identical to those in rabbit. Similarities include dose-dependent decreases in cAMP levels stimulated by forskolin elicited by 1-(3-chlorophenyl) piperazine (mCPP), 5-CT and 8-OH-DPAT. Moreover, the inhibitory effect of 5-CT can also be significantly reduced by the 5-HT1 receptor antagonist, propranolol. However, unlike the case of rabbit tissue, spiperone was ineffective in abolishing the 5-CT response in human ICB homogenates.

Original languageEnglish
Pages (from-to)209-216
Number of pages8
JournalExperimental Eye Research
Volume57
Issue number2
DOIs
Publication statusPublished - 1 Jan 1993
Externally publishedYes

Fingerprint

Serotonin 5-HT1 Receptors
Iris
Adenylyl Cyclases
Ciliary Body
Rabbits
Serotonin
Colforsin
Spiperone
8-Hydroxy-2-(di-n-propylamino)tetralin
tropisetron
Receptor, Serotonin, 5-HT1A
Propranolol
Serotonin 5-HT1 Receptor Antagonists
Serotonin 5-HT1 Receptor Agonists
Serotonin 5-HT2 Receptor Antagonists
Ketanserin
Cyclic AMP
5-carboxamidotryptamine

Cite this

@article{664ba97ff64f4019acf0c1ad952eb9ed,
title = "The presence of serotonin (5-HT1) receptors negatively coupled to adenylate cyclase in rabbit and human iris-ciliary processes",
abstract = "Serotonin (5-hydroxytryptamine, 5-HT) reduces forskolin-induced stimulation of cyclic AMP in rabbit iris-ciliary body (ICB) homogenates. The effect is dose dependent and can be mimicked by a number of 5-HT1 receptor agonists including 5-carboxamidotryptamine (5-CT) and RU 24969 [5-methoxy-3-(1,2,3,6-tetrahydro-4-pyridinyl)-1-indole]. The inhibitory effects 5-CT and the 5-HT(1A) selective agent 8-hydroxy-2-(di-n-propyl-amino) tetralin (8-OH-DPAT) on forskolin stimulated adenylate cyclase activity are greater in isolated ciliary processes than in the iris musculature. Spiperone and propranolol significantly antagonize the action of 5-CT in the iris-ciliary body, while ketanserin (5-HT2 antagonist) and ICS 205930 (5-HT(3/4 blocker) were without influence, indicating the presence of the 5-HT(1A) subtype of receptor. Studies carried out on human ICB homogenates also suggest the presence of 5-HT(1A)-like receptors, although these receptors are not identical to those in rabbit. Similarities include dose-dependent decreases in cAMP levels stimulated by forskolin elicited by 1-(3-chlorophenyl) piperazine (mCPP), 5-CT and 8-OH-DPAT. Moreover, the inhibitory effect of 5-CT can also be significantly reduced by the 5-HT1 receptor antagonist, propranolol. However, unlike the case of rabbit tissue, spiperone was ineffective in abolishing the 5-CT response in human ICB homogenates.",
author = "Barnett, {Nigel L.} and Osborne, {Neville N.}",
year = "1993",
month = "1",
day = "1",
doi = "10.1006/exer.1993.1116",
language = "English",
volume = "57",
pages = "209--216",
journal = "Experimental Eye Research",
issn = "0014-4835",
publisher = "Academic Press Inc.",
number = "2",

}

The presence of serotonin (5-HT1) receptors negatively coupled to adenylate cyclase in rabbit and human iris-ciliary processes. / Barnett, Nigel L.; Osborne, Neville N.

In: Experimental Eye Research, Vol. 57, No. 2, 01.01.1993, p. 209-216.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - The presence of serotonin (5-HT1) receptors negatively coupled to adenylate cyclase in rabbit and human iris-ciliary processes

AU - Barnett, Nigel L.

AU - Osborne, Neville N.

PY - 1993/1/1

Y1 - 1993/1/1

N2 - Serotonin (5-hydroxytryptamine, 5-HT) reduces forskolin-induced stimulation of cyclic AMP in rabbit iris-ciliary body (ICB) homogenates. The effect is dose dependent and can be mimicked by a number of 5-HT1 receptor agonists including 5-carboxamidotryptamine (5-CT) and RU 24969 [5-methoxy-3-(1,2,3,6-tetrahydro-4-pyridinyl)-1-indole]. The inhibitory effects 5-CT and the 5-HT(1A) selective agent 8-hydroxy-2-(di-n-propyl-amino) tetralin (8-OH-DPAT) on forskolin stimulated adenylate cyclase activity are greater in isolated ciliary processes than in the iris musculature. Spiperone and propranolol significantly antagonize the action of 5-CT in the iris-ciliary body, while ketanserin (5-HT2 antagonist) and ICS 205930 (5-HT(3/4 blocker) were without influence, indicating the presence of the 5-HT(1A) subtype of receptor. Studies carried out on human ICB homogenates also suggest the presence of 5-HT(1A)-like receptors, although these receptors are not identical to those in rabbit. Similarities include dose-dependent decreases in cAMP levels stimulated by forskolin elicited by 1-(3-chlorophenyl) piperazine (mCPP), 5-CT and 8-OH-DPAT. Moreover, the inhibitory effect of 5-CT can also be significantly reduced by the 5-HT1 receptor antagonist, propranolol. However, unlike the case of rabbit tissue, spiperone was ineffective in abolishing the 5-CT response in human ICB homogenates.

AB - Serotonin (5-hydroxytryptamine, 5-HT) reduces forskolin-induced stimulation of cyclic AMP in rabbit iris-ciliary body (ICB) homogenates. The effect is dose dependent and can be mimicked by a number of 5-HT1 receptor agonists including 5-carboxamidotryptamine (5-CT) and RU 24969 [5-methoxy-3-(1,2,3,6-tetrahydro-4-pyridinyl)-1-indole]. The inhibitory effects 5-CT and the 5-HT(1A) selective agent 8-hydroxy-2-(di-n-propyl-amino) tetralin (8-OH-DPAT) on forskolin stimulated adenylate cyclase activity are greater in isolated ciliary processes than in the iris musculature. Spiperone and propranolol significantly antagonize the action of 5-CT in the iris-ciliary body, while ketanserin (5-HT2 antagonist) and ICS 205930 (5-HT(3/4 blocker) were without influence, indicating the presence of the 5-HT(1A) subtype of receptor. Studies carried out on human ICB homogenates also suggest the presence of 5-HT(1A)-like receptors, although these receptors are not identical to those in rabbit. Similarities include dose-dependent decreases in cAMP levels stimulated by forskolin elicited by 1-(3-chlorophenyl) piperazine (mCPP), 5-CT and 8-OH-DPAT. Moreover, the inhibitory effect of 5-CT can also be significantly reduced by the 5-HT1 receptor antagonist, propranolol. However, unlike the case of rabbit tissue, spiperone was ineffective in abolishing the 5-CT response in human ICB homogenates.

UR - http://www.scopus.com/inward/record.url?scp=0027304897&partnerID=8YFLogxK

U2 - 10.1006/exer.1993.1116

DO - 10.1006/exer.1993.1116

M3 - Article

VL - 57

SP - 209

EP - 216

JO - Experimental Eye Research

JF - Experimental Eye Research

SN - 0014-4835

IS - 2

ER -