The identification of diurnal variations on circulating immune cells by finger prick blood sampling in small sample sizes: a pilot study

Dayna Bushell; Jonathan Kah Huat Tan; Jessica Smith; Christian Moro

doi:10.1093/labmed/lmad062

The identification of diurnal variations on circulating immune cells by finger prick blood sampling in small sample sizes: a pilot study

Dayna Bushell, Jonathan Kah Huat Tan, Jessica Smith, Christian Moro

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Abstract

OBJECTIVE: There are well-described impacts of biological rhythms on human physiology. With the increasing push for routine blood tests for preventative medical care and clinical and physiological research, optimizing effectiveness is paramount. This study aimed to determine whether it is feasible to assess diurnal variations of peripheral lymphocyte prevalence using finger prick blood in a small sample size.

METHODS: Using polychromatic flow cytometry, the prevalence of lymphocytes was assessed using 25 µL fingertip blood samples at 8 AM and 5 PM from 8 participants.

RESULTS: TH cells and B cells showed significantly higher percentages in the 5 PM samples, whereas NK cells demonstrated a significantly higher morning percentage. T cells, leukocytes, and cytotoxic T cells showed no significant changes.

CONCLUSION: The detection of diurnal variations demonstrates that small blood volumes can be used to detect lymphocyte variations. The lower blood volume required provides a new testing method for clinical and research settings.

Original language	English
Pages (from-to)	220-226
Number of pages	7
Journal	Laboratory Medicine
Volume	55
Issue number	2
Early online date	21 Jul 2023
DOIs	https://doi.org/10.1093/labmed/lmad062
Publication status	Published - 1 Mar 2024

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The identification of diurnal variations on circulating immune cells by finger prick blood sampling in small sample sizes: a pilot study Final published version, 903 KBLicence: CC BY

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abstract = "OBJECTIVE: There are well-described impacts of biological rhythms on human physiology. With the increasing push for routine blood tests for preventative medical care and clinical and physiological research, optimizing effectiveness is paramount. This study aimed to determine whether it is feasible to assess diurnal variations of peripheral lymphocyte prevalence using finger prick blood in a small sample size.METHODS: Using polychromatic flow cytometry, the prevalence of lymphocytes was assessed using 25 µL fingertip blood samples at 8 AM and 5 PM from 8 participants.RESULTS: TH cells and B cells showed significantly higher percentages in the 5 PM samples, whereas NK cells demonstrated a significantly higher morning percentage. T cells, leukocytes, and cytotoxic T cells showed no significant changes.CONCLUSION: The detection of diurnal variations demonstrates that small blood volumes can be used to detect lymphocyte variations. The lower blood volume required provides a new testing method for clinical and research settings.",

author = "Dayna Bushell and Tan, {Jonathan Kah Huat} and Jessica Smith and Christian Moro",

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doi = "10.1093/labmed/lmad062",

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T1 - The identification of diurnal variations on circulating immune cells by finger prick blood sampling in small sample sizes: a pilot study

AU - Bushell, Dayna

AU - Tan, Jonathan Kah Huat

AU - Smith, Jessica

AU - Moro, Christian

PY - 2024/3/1

Y1 - 2024/3/1

N2 - OBJECTIVE: There are well-described impacts of biological rhythms on human physiology. With the increasing push for routine blood tests for preventative medical care and clinical and physiological research, optimizing effectiveness is paramount. This study aimed to determine whether it is feasible to assess diurnal variations of peripheral lymphocyte prevalence using finger prick blood in a small sample size.METHODS: Using polychromatic flow cytometry, the prevalence of lymphocytes was assessed using 25 µL fingertip blood samples at 8 AM and 5 PM from 8 participants.RESULTS: TH cells and B cells showed significantly higher percentages in the 5 PM samples, whereas NK cells demonstrated a significantly higher morning percentage. T cells, leukocytes, and cytotoxic T cells showed no significant changes.CONCLUSION: The detection of diurnal variations demonstrates that small blood volumes can be used to detect lymphocyte variations. The lower blood volume required provides a new testing method for clinical and research settings.

AB - OBJECTIVE: There are well-described impacts of biological rhythms on human physiology. With the increasing push for routine blood tests for preventative medical care and clinical and physiological research, optimizing effectiveness is paramount. This study aimed to determine whether it is feasible to assess diurnal variations of peripheral lymphocyte prevalence using finger prick blood in a small sample size.METHODS: Using polychromatic flow cytometry, the prevalence of lymphocytes was assessed using 25 µL fingertip blood samples at 8 AM and 5 PM from 8 participants.RESULTS: TH cells and B cells showed significantly higher percentages in the 5 PM samples, whereas NK cells demonstrated a significantly higher morning percentage. T cells, leukocytes, and cytotoxic T cells showed no significant changes.CONCLUSION: The detection of diurnal variations demonstrates that small blood volumes can be used to detect lymphocyte variations. The lower blood volume required provides a new testing method for clinical and research settings.

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The identification of diurnal variations on circulating immune cells by finger prick blood sampling in small sample sizes: a pilot study

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