The identification and characterisation of articular cartilage progenitor cells

C. W. Archer, S Redman, Joanna Bishop, S Bowyer, GP Dowthwaite

Research output: Contribution to conferenceAbstractResearchpeer-review

Abstract

Introduction: There are two major problems which afflict current strategies in
cartilage repair. One problem is tissue integration between host and reparative
tissue. The second problem is the generation of a repair tissue with the
structural characteristics of articular cartilage. Using the marsupial
Monodelphis domestica as a model system, it has been shown that articular
cartilage grows by apposition from the articular surface towards the
subchondral bone and that this growth is driven by the proliferation of surface
zone cells (1, 2), Additionally, a population of cells with an increased cell
cycle time was identified within the surface zone; a property typical of many
progenitor cell populations (2). The aim of our research is to identify and
characterise a chondroprogenitor population from articular cartilage to enable
the rapid culture of undifferentiated chondrocytes in vitro for future clinical
use. Here we describe the isolation and partial characterisation of a cell
population from the articular surface which exhibits differential adhesion to
fibronectin, differential integrin expression and the ability to form large
numbers of colonies from an initialy small seeding density; properties that are
common to known progenitor cell populations of other tissues. Additionally
we report on the presence of the cell surface signalling molecule Notch 1 (N1)
in a subpopulation of surface zone chondrocytes and that this N1-expressing
subpopulation has an enhanced ability to form large numbers of colonies from
an initially low seeding density

Paper No: 0009
Original languageEnglish
Publication statusPublished - Feb 2002
Externally publishedYes
EventThe 48th Annual Meeting of the Orthopaedic Research Society - Dallas, United States
Duration: 10 Feb 200213 Feb 2002

Conference

ConferenceThe 48th Annual Meeting of the Orthopaedic Research Society
CountryUnited States
CityDallas
Period10/02/0213/02/02

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Articular Cartilage
Stem Cells
Chondrocytes
Population
Joints
Bone Development
Integrins
Cell Proliferation
Research

Cite this

Archer, C. W., Redman, S., Bishop, J., Bowyer, S., & Dowthwaite, GP. (2002). The identification and characterisation of articular cartilage progenitor cells. Abstract from The 48th Annual Meeting of the Orthopaedic Research Society, Dallas, United States.
Archer, C. W. ; Redman, S ; Bishop, Joanna ; Bowyer, S ; Dowthwaite, GP. / The identification and characterisation of articular cartilage progenitor cells. Abstract from The 48th Annual Meeting of the Orthopaedic Research Society, Dallas, United States.
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abstract = "Introduction: There are two major problems which afflict current strategies incartilage repair. One problem is tissue integration between host and reparativetissue. The second problem is the generation of a repair tissue with thestructural characteristics of articular cartilage. Using the marsupialMonodelphis domestica as a model system, it has been shown that articularcartilage grows by apposition from the articular surface towards thesubchondral bone and that this growth is driven by the proliferation of surfacezone cells (1, 2), Additionally, a population of cells with an increased cellcycle time was identified within the surface zone; a property typical of manyprogenitor cell populations (2). The aim of our research is to identify andcharacterise a chondroprogenitor population from articular cartilage to enablethe rapid culture of undifferentiated chondrocytes in vitro for future clinicaluse. Here we describe the isolation and partial characterisation of a cellpopulation from the articular surface which exhibits differential adhesion tofibronectin, differential integrin expression and the ability to form largenumbers of colonies from an initialy small seeding density; properties that arecommon to known progenitor cell populations of other tissues. Additionallywe report on the presence of the cell surface signalling molecule Notch 1 (N1)in a subpopulation of surface zone chondrocytes and that this N1-expressingsubpopulation has an enhanced ability to form large numbers of colonies froman initially low seeding densityPaper No: 0009",
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Archer, CW, Redman, S, Bishop, J, Bowyer, S & Dowthwaite, GP 2002, 'The identification and characterisation of articular cartilage progenitor cells' The 48th Annual Meeting of the Orthopaedic Research Society, Dallas, United States, 10/02/02 - 13/02/02, .

The identification and characterisation of articular cartilage progenitor cells. / Archer, C. W.; Redman, S; Bishop, Joanna ; Bowyer, S; Dowthwaite, GP.

2002. Abstract from The 48th Annual Meeting of the Orthopaedic Research Society, Dallas, United States.

Research output: Contribution to conferenceAbstractResearchpeer-review

TY - CONF

T1 - The identification and characterisation of articular cartilage progenitor cells

AU - Archer, C. W.

AU - Redman, S

AU - Bishop, Joanna

AU - Bowyer, S

AU - Dowthwaite, GP

PY - 2002/2

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N2 - Introduction: There are two major problems which afflict current strategies incartilage repair. One problem is tissue integration between host and reparativetissue. The second problem is the generation of a repair tissue with thestructural characteristics of articular cartilage. Using the marsupialMonodelphis domestica as a model system, it has been shown that articularcartilage grows by apposition from the articular surface towards thesubchondral bone and that this growth is driven by the proliferation of surfacezone cells (1, 2), Additionally, a population of cells with an increased cellcycle time was identified within the surface zone; a property typical of manyprogenitor cell populations (2). The aim of our research is to identify andcharacterise a chondroprogenitor population from articular cartilage to enablethe rapid culture of undifferentiated chondrocytes in vitro for future clinicaluse. Here we describe the isolation and partial characterisation of a cellpopulation from the articular surface which exhibits differential adhesion tofibronectin, differential integrin expression and the ability to form largenumbers of colonies from an initialy small seeding density; properties that arecommon to known progenitor cell populations of other tissues. Additionallywe report on the presence of the cell surface signalling molecule Notch 1 (N1)in a subpopulation of surface zone chondrocytes and that this N1-expressingsubpopulation has an enhanced ability to form large numbers of colonies froman initially low seeding densityPaper No: 0009

AB - Introduction: There are two major problems which afflict current strategies incartilage repair. One problem is tissue integration between host and reparativetissue. The second problem is the generation of a repair tissue with thestructural characteristics of articular cartilage. Using the marsupialMonodelphis domestica as a model system, it has been shown that articularcartilage grows by apposition from the articular surface towards thesubchondral bone and that this growth is driven by the proliferation of surfacezone cells (1, 2), Additionally, a population of cells with an increased cellcycle time was identified within the surface zone; a property typical of manyprogenitor cell populations (2). The aim of our research is to identify andcharacterise a chondroprogenitor population from articular cartilage to enablethe rapid culture of undifferentiated chondrocytes in vitro for future clinicaluse. Here we describe the isolation and partial characterisation of a cellpopulation from the articular surface which exhibits differential adhesion tofibronectin, differential integrin expression and the ability to form largenumbers of colonies from an initialy small seeding density; properties that arecommon to known progenitor cell populations of other tissues. Additionallywe report on the presence of the cell surface signalling molecule Notch 1 (N1)in a subpopulation of surface zone chondrocytes and that this N1-expressingsubpopulation has an enhanced ability to form large numbers of colonies froman initially low seeding densityPaper No: 0009

M3 - Abstract

ER -

Archer CW, Redman S, Bishop J, Bowyer S, Dowthwaite GP. The identification and characterisation of articular cartilage progenitor cells. 2002. Abstract from The 48th Annual Meeting of the Orthopaedic Research Society, Dallas, United States.