Abstract
Introduction: There are two major problems which afflict current strategies in
cartilage repair. One problem is tissue integration between host and reparative
tissue. The second problem is the generation of a repair tissue with the
structural characteristics of articular cartilage. Using the marsupial
Monodelphis domestica as a model system, it has been shown that articular
cartilage grows by apposition from the articular surface towards the
subchondral bone and that this growth is driven by the proliferation of surface
zone cells (1, 2), Additionally, a population of cells with an increased cell
cycle time was identified within the surface zone; a property typical of many
progenitor cell populations (2). The aim of our research is to identify and
characterise a chondroprogenitor population from articular cartilage to enable
the rapid culture of undifferentiated chondrocytes in vitro for future clinical
use. Here we describe the isolation and partial characterisation of a cell
population from the articular surface which exhibits differential adhesion to
fibronectin, differential integrin expression and the ability to form large
numbers of colonies from an initialy small seeding density; properties that are
common to known progenitor cell populations of other tissues. Additionally
we report on the presence of the cell surface signalling molecule Notch 1 (N1)
in a subpopulation of surface zone chondrocytes and that this N1-expressing
subpopulation has an enhanced ability to form large numbers of colonies from
an initially low seeding density
Paper No: 0009
cartilage repair. One problem is tissue integration between host and reparative
tissue. The second problem is the generation of a repair tissue with the
structural characteristics of articular cartilage. Using the marsupial
Monodelphis domestica as a model system, it has been shown that articular
cartilage grows by apposition from the articular surface towards the
subchondral bone and that this growth is driven by the proliferation of surface
zone cells (1, 2), Additionally, a population of cells with an increased cell
cycle time was identified within the surface zone; a property typical of many
progenitor cell populations (2). The aim of our research is to identify and
characterise a chondroprogenitor population from articular cartilage to enable
the rapid culture of undifferentiated chondrocytes in vitro for future clinical
use. Here we describe the isolation and partial characterisation of a cell
population from the articular surface which exhibits differential adhesion to
fibronectin, differential integrin expression and the ability to form large
numbers of colonies from an initialy small seeding density; properties that are
common to known progenitor cell populations of other tissues. Additionally
we report on the presence of the cell surface signalling molecule Notch 1 (N1)
in a subpopulation of surface zone chondrocytes and that this N1-expressing
subpopulation has an enhanced ability to form large numbers of colonies from
an initially low seeding density
Paper No: 0009
| Original language | English |
|---|---|
| Publication status | Published - Feb 2002 |
| Externally published | Yes |
| Event | The 48th Annual Meeting of the Orthopaedic Research Society - Dallas, United States Duration: 10 Feb 2002 → 13 Feb 2002 |
Conference
| Conference | The 48th Annual Meeting of the Orthopaedic Research Society |
|---|---|
| Country/Territory | United States |
| City | Dallas |
| Period | 10/02/02 → 13/02/02 |