Abstract
Objective: Nerve tissue engineering is one of the most promising approaches to nerve repair and regeneration. Nanofibrous scaffolds play a pivotal role in tissue engineering.
The orientation of nanofibers is one of the important features of a perfect tissue scaffold, because the fiber orientation greatly influences cell growth and related functions in cells such as nerve.
Materials and Methods: In this regard, aligned and random electrospun poly ( e-caprolactone) (PCL) fibers were fabricated to test their potential to provide contact guidance to neural cell from mesenchymal stem cell. Then the chemical and mechanical characterizations of nanofibers were carried out using scanning electron microscopy (SEM), contact angle and tensile instrument. The differentiation of MSCs was carried out using neuronal inducing factors including basic fibroblast growth factor, nerve growth factor and brain derived growth factor in DMEM/F12 media. The differentiation and neurite outgrowth were analyzed by immunocytochemistry and SEM.
Results: Scanning electron microscopy results showed that the direction of cells elongation is parallel to the direction of PCL fibers for aligned scaffolds. The differentiated Mesenchymal cells on nanofiber scaffold were found to express the neuronal proteins, b-tubulin III and Map2 on day 15 after culture. Cell cytoskeleton and nuclei were observed to align and elongate along the fiber axes on aligned nanofibrous scaffolds.
Conclusion: These results suggested that the aligned electrospun nanofibers are capable of controlling the orientation of neurons and acted as a positive cue to support neurite outgrowth.
The orientation of nanofibers is one of the important features of a perfect tissue scaffold, because the fiber orientation greatly influences cell growth and related functions in cells such as nerve.
Materials and Methods: In this regard, aligned and random electrospun poly ( e-caprolactone) (PCL) fibers were fabricated to test their potential to provide contact guidance to neural cell from mesenchymal stem cell. Then the chemical and mechanical characterizations of nanofibers were carried out using scanning electron microscopy (SEM), contact angle and tensile instrument. The differentiation of MSCs was carried out using neuronal inducing factors including basic fibroblast growth factor, nerve growth factor and brain derived growth factor in DMEM/F12 media. The differentiation and neurite outgrowth were analyzed by immunocytochemistry and SEM.
Results: Scanning electron microscopy results showed that the direction of cells elongation is parallel to the direction of PCL fibers for aligned scaffolds. The differentiated Mesenchymal cells on nanofiber scaffold were found to express the neuronal proteins, b-tubulin III and Map2 on day 15 after culture. Cell cytoskeleton and nuclei were observed to align and elongate along the fiber axes on aligned nanofibrous scaffolds.
Conclusion: These results suggested that the aligned electrospun nanofibers are capable of controlling the orientation of neurons and acted as a positive cue to support neurite outgrowth.
| Original language | English |
|---|---|
| Pages (from-to) | 37 |
| Journal | Cell Journal |
| Volume | 13 |
| Issue number | Supplement 3 |
| Publication status | Published - 7 Sept 2011 |
| Externally published | Yes |