The effect of structural offset and gradient pore size on Osteoblast differentiation in PCL scaffolds

Naghmeh Abbasi, Stephen Hamlet, Nathan Castro, Abdalla Abdal-Hay, Saso Ivanovski

Research output: Contribution to conferencePosterResearchpeer-review


Melt elctrospinning writing (MEW) is a solvent free
strategy allowing precise control of the configuration of
electrospun fibers in 3D printed scaffolds 1. By controlling
fibre diameter and geometry and the distribution and
size of pores, scaffolds with increased surface area
allowing, greater cell penetration and proliferation,
nutrient/waste/gas exchange and improved mechanical
properties, can be produced 2. This study describes the
effect of 1) pore size, 2) pore size gradient and 3) fibre
offset on osteogenesis in MEW 3D -poly caprolactone
(PCL) scaffolds. The highest surface area was seen
with offset scaffolds and calcium phosphate coating
improved scaffold wettability. Mineralization, bonespecific gene and protein expression were assessed
following 30 days cell culture with human osteoblasts.
The 50% offset scaffold showed higher mineralization
in response to osteogenic growth factors while high
levels of expression of opn and ocn markers of bone
differentiation were observed in the gradient scaffold.
All groups showed upregulation of alp and collagen-I
after 14 days of osteogenic differentiation. In conclusion,
the pore size gradient and offset scaffolds can be
considered good candidates for bone formation studies.
Original languageEnglish
Publication statusPublished - 20 Jun 2018
Externally publishedYes
EventInternational Society for Stem Cell Research Annual Meeting 2018 - Melbourne Convention Centre, Melbourne, Australia
Duration: 20 Jun 201823 Jun 2018


ConferenceInternational Society for Stem Cell Research Annual Meeting 2018
Abbreviated titleISSCR
OtherThe Annual Meeting of the International Society for Stem Cell Research brings together world
leading experts to discuss recent findings and progress that is driving the development of stem
cell treatments and cures for disease. For our world-renowned researchers, closer ties with
international researchers mean more opportunities to partner with the best in the world and
collaborate on medical breakthroughs that will change lives here and abroad.
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