The effect of growth hormone administration on the regulation of mitochondrial apoptosis in-vivo

James Keane, Lotti Tajouri, Bon Gray

Research output: Contribution to journalArticleResearchpeer-review

8 Citations (Scopus)

Abstract

The purpose of this study was to determine whether recombinant human growth hormone (rhGH) would show any significant effects on the expression of apoptosis regulating proteins in peripheral blood mononuclear cells (PBMCs). Additionally, the potential for post-transcriptional regulation of gene expression by miRNA was assessed in two cellular compartments, the cytosol and the mitochondria. Ten male subjects were subcutaneously injected with either rhGH (1 mg) or saline (0.9%) for seven consecutive days in a double-blinded fashion. Blood sampling was undertaken prior to treatment administration and over a period of three weeks following treatment cessation. Bcl-2 and Bak gene and protein expression levels were measured in PBMCs, while attention was also directed to the expression of miR-181a and miR-125b, known translational inhibitors of Bcl-2 and Bak respectively. Results showed that rhGH significantly decreased Bak protein concentrations compared to placebo samples for up to 8 days post treatment. While cytosolic miRNA expression was not found to be significantly affected by rhGH, measurement of the expression of miR-125b in mitochondrial fractions showed a significant down-regulation eight days post-rhGH administration. These findings suggest that rhGH induces short-term anti-apoptotic effects which may be partially mediated through a novel pathway that alters the concentration of mitochondrially-associated miRNAs.

Original languageEnglish
Pages (from-to)12753-12772
Number of pages20
JournalInternational Journal of Molecular Sciences
Volume16
Issue number6
DOIs
Publication statusPublished - 5 Jun 2015

Fingerprint

pituitary hormones
Human Growth Hormone
Hormones
apoptosis
Cell death
Growth Hormone
Apoptosis
bcl-2 Homologous Antagonist-Killer Protein
MicroRNAs
blood cells
Blood
gene expression
proteins
Proteins
Blood Cells
bcl-2 Genes
Withholding Treatment
Mitochondria
mitochondria
Gene Expression Regulation

Cite this

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abstract = "The purpose of this study was to determine whether recombinant human growth hormone (rhGH) would show any significant effects on the expression of apoptosis regulating proteins in peripheral blood mononuclear cells (PBMCs). Additionally, the potential for post-transcriptional regulation of gene expression by miRNA was assessed in two cellular compartments, the cytosol and the mitochondria. Ten male subjects were subcutaneously injected with either rhGH (1 mg) or saline (0.9{\%}) for seven consecutive days in a double-blinded fashion. Blood sampling was undertaken prior to treatment administration and over a period of three weeks following treatment cessation. Bcl-2 and Bak gene and protein expression levels were measured in PBMCs, while attention was also directed to the expression of miR-181a and miR-125b, known translational inhibitors of Bcl-2 and Bak respectively. Results showed that rhGH significantly decreased Bak protein concentrations compared to placebo samples for up to 8 days post treatment. While cytosolic miRNA expression was not found to be significantly affected by rhGH, measurement of the expression of miR-125b in mitochondrial fractions showed a significant down-regulation eight days post-rhGH administration. These findings suggest that rhGH induces short-term anti-apoptotic effects which may be partially mediated through a novel pathway that alters the concentration of mitochondrially-associated miRNAs.",
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The effect of growth hormone administration on the regulation of mitochondrial apoptosis in-vivo. / Keane, James; Tajouri, Lotti; Gray, Bon.

In: International Journal of Molecular Sciences, Vol. 16, No. 6, 05.06.2015, p. 12753-12772.

Research output: Contribution to journalArticleResearchpeer-review

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