Bladder dysfunction can lead to a range of debilitating conditions and treatments are oftenassociated with several side effects that substantially reduce quality of life. Research intoalternative targets acting on bladder layers, including 5-hydroxytryptamine (5-HT), istherefore extremely valuable. The aim of this study was to determine the effects of 5-HT onthe contractile activity of the urothelium and lamina propria, and the likely mediatingreceptor. Strips of porcine urothelium and lamina propria were suspended in 10mL organbaths filled with warm Krebs-bicarbonate solution. Cumulative dose-response curves using 5-Hydroxytryptamine were generated in the presence of the 5-HT antagonists, methiothepin,ketanserin, ondansetron and GR113808. Maximal tissue contractions were determined withcarbachol doses. Data was analysed using a paired Student’s t-test. 5-HT applicationproduced concentration-dependent contractions in the tissue. Maximal contractions to 5-HT(0.94±0.08g) were approximately 17% of carbachol-mediated contractions (n=37, 30?M). 5-HT stimulated contractions were unchanged in the presence of the nitric oxide synthesisinhibitor L-NNA (n=5, 100?M) or cyclooxygenase inhibitor indomethacin (n=4, 100?M). Inthe presence of the 5-HT1 antagonist responses were significantly (p?0.001) decreased (n=8,10nM). Ketanserin (n=9, 30nM), ondansetron (n=13, 30nM) and GR113808 (n=8, 100nM)produced no significant changes in the 5-HT contractile response (all p=NSD). 5-HTproduced concentration-dependent contractions in porcine urothelium and lamina propria andthis response was unlikely to be mediated by receptors 5-HT2, 5-HT3 or 5-HT4. Preliminaryresponses appear to indicate involvement of 5-HT1 receptors. This study shows 5-HTreceptors may be a viable option for bladder dysfunction research and treatment.
|Publication status||Published - 2015|
|Event||The7th National Symposium on Advances in Gastrointestinal & Urogenital Research - Adelaide, Australia|
Duration: 2 Oct 2015 → …
|Conference||The7th National Symposium on Advances in Gastrointestinal & Urogenital Research|
|Period||2/10/15 → …|