The contractile effects of 5-hydroxytryptamine on porcine bladder urothelium and lamina propria

Elizabeth J Edwards, Russ Chess-Williams, Christian Moro

Research output: Contribution to conferenceAbstractResearch

Abstract

Bladder dysfunction can lead to a range of debilitating conditions and treatments are oftenassociated with several side effects that substantially reduce quality of life. Research intoalternative targets acting on bladder layers, including 5-hydroxytryptamine (5-HT), istherefore extremely valuable. The aim of this study was to determine the effects of 5-HT onthe contractile activity of the urothelium and lamina propria, and the likely mediatingreceptor. Strips of porcine urothelium and lamina propria were suspended in 10mL organbaths filled with warm Krebs-bicarbonate solution. Cumulative dose-response curves using 5-Hydroxytryptamine were generated in the presence of the 5-HT antagonists, methiothepin,ketanserin, ondansetron and GR113808. Maximal tissue contractions were determined withcarbachol doses. Data was analysed using a paired Student’s t-test. 5-HT applicationproduced concentration-dependent contractions in the tissue. Maximal contractions to 5-HT(0.94±0.08g) were approximately 17% of carbachol-mediated contractions (n=37, 30?M). 5-HT stimulated contractions were unchanged in the presence of the nitric oxide synthesisinhibitor L-NNA (n=5, 100?M) or cyclooxygenase inhibitor indomethacin (n=4, 100?M). Inthe presence of the 5-HT1 antagonist responses were significantly (p?0.001) decreased (n=8,10nM). Ketanserin (n=9, 30nM), ondansetron (n=13, 30nM) and GR113808 (n=8, 100nM)produced no significant changes in the 5-HT contractile response (all p=NSD). 5-HTproduced concentration-dependent contractions in porcine urothelium and lamina propria andthis response was unlikely to be mediated by receptors 5-HT2, 5-HT3 or 5-HT4. Preliminaryresponses appear to indicate involvement of 5-HT1 receptors. This study shows 5-HTreceptors may be a viable option for bladder dysfunction research and treatment.
Original languageEnglish
Publication statusPublished - 2015
EventThe7th National Symposium on Advances in Gastrointestinal & Urogenital Research - Adelaide, Australia
Duration: 2 Oct 2015 → …

Conference

ConferenceThe7th National Symposium on Advances in Gastrointestinal & Urogenital Research
CountryAustralia
CityAdelaide
Period2/10/15 → …

Fingerprint

Urothelium
Serotonin
Mucous Membrane
Urinary Bladder
Swine
Ondansetron
Ketanserin
Methiothepin
Serotonin 5-HT1 Receptors
Serotonin 5-HT1 Receptor Antagonists
Serotonin Antagonists
Cyclooxygenase Inhibitors
Carbachol
Bicarbonates
Research
Indomethacin
Nitric Oxide
Quality of Life
Students
Therapeutics

Cite this

Edwards, E. J., Chess-Williams, R., & Moro, C. (2015). The contractile effects of 5-hydroxytryptamine on porcine bladder urothelium and lamina propria. Abstract from The7th National Symposium on Advances in Gastrointestinal & Urogenital Research , Adelaide, Australia.
Edwards, Elizabeth J ; Chess-Williams, Russ ; Moro, Christian. / The contractile effects of 5-hydroxytryptamine on porcine bladder urothelium and lamina propria. Abstract from The7th National Symposium on Advances in Gastrointestinal & Urogenital Research , Adelaide, Australia.
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Edwards, EJ, Chess-Williams, R & Moro, C 2015, 'The contractile effects of 5-hydroxytryptamine on porcine bladder urothelium and lamina propria' The7th National Symposium on Advances in Gastrointestinal & Urogenital Research , Adelaide, Australia, 2/10/15, .

The contractile effects of 5-hydroxytryptamine on porcine bladder urothelium and lamina propria. / Edwards, Elizabeth J; Chess-Williams, Russ; Moro, Christian.

2015. Abstract from The7th National Symposium on Advances in Gastrointestinal & Urogenital Research , Adelaide, Australia.

Research output: Contribution to conferenceAbstractResearch

TY - CONF

T1 - The contractile effects of 5-hydroxytryptamine on porcine bladder urothelium and lamina propria

AU - Edwards, Elizabeth J

AU - Chess-Williams, Russ

AU - Moro, Christian

PY - 2015

Y1 - 2015

N2 - Bladder dysfunction can lead to a range of debilitating conditions and treatments are oftenassociated with several side effects that substantially reduce quality of life. Research intoalternative targets acting on bladder layers, including 5-hydroxytryptamine (5-HT), istherefore extremely valuable. The aim of this study was to determine the effects of 5-HT onthe contractile activity of the urothelium and lamina propria, and the likely mediatingreceptor. Strips of porcine urothelium and lamina propria were suspended in 10mL organbaths filled with warm Krebs-bicarbonate solution. Cumulative dose-response curves using 5-Hydroxytryptamine were generated in the presence of the 5-HT antagonists, methiothepin,ketanserin, ondansetron and GR113808. Maximal tissue contractions were determined withcarbachol doses. Data was analysed using a paired Student’s t-test. 5-HT applicationproduced concentration-dependent contractions in the tissue. Maximal contractions to 5-HT(0.94±0.08g) were approximately 17% of carbachol-mediated contractions (n=37, 30?M). 5-HT stimulated contractions were unchanged in the presence of the nitric oxide synthesisinhibitor L-NNA (n=5, 100?M) or cyclooxygenase inhibitor indomethacin (n=4, 100?M). Inthe presence of the 5-HT1 antagonist responses were significantly (p?0.001) decreased (n=8,10nM). Ketanserin (n=9, 30nM), ondansetron (n=13, 30nM) and GR113808 (n=8, 100nM)produced no significant changes in the 5-HT contractile response (all p=NSD). 5-HTproduced concentration-dependent contractions in porcine urothelium and lamina propria andthis response was unlikely to be mediated by receptors 5-HT2, 5-HT3 or 5-HT4. Preliminaryresponses appear to indicate involvement of 5-HT1 receptors. This study shows 5-HTreceptors may be a viable option for bladder dysfunction research and treatment.

AB - Bladder dysfunction can lead to a range of debilitating conditions and treatments are oftenassociated with several side effects that substantially reduce quality of life. Research intoalternative targets acting on bladder layers, including 5-hydroxytryptamine (5-HT), istherefore extremely valuable. The aim of this study was to determine the effects of 5-HT onthe contractile activity of the urothelium and lamina propria, and the likely mediatingreceptor. Strips of porcine urothelium and lamina propria were suspended in 10mL organbaths filled with warm Krebs-bicarbonate solution. Cumulative dose-response curves using 5-Hydroxytryptamine were generated in the presence of the 5-HT antagonists, methiothepin,ketanserin, ondansetron and GR113808. Maximal tissue contractions were determined withcarbachol doses. Data was analysed using a paired Student’s t-test. 5-HT applicationproduced concentration-dependent contractions in the tissue. Maximal contractions to 5-HT(0.94±0.08g) were approximately 17% of carbachol-mediated contractions (n=37, 30?M). 5-HT stimulated contractions were unchanged in the presence of the nitric oxide synthesisinhibitor L-NNA (n=5, 100?M) or cyclooxygenase inhibitor indomethacin (n=4, 100?M). Inthe presence of the 5-HT1 antagonist responses were significantly (p?0.001) decreased (n=8,10nM). Ketanserin (n=9, 30nM), ondansetron (n=13, 30nM) and GR113808 (n=8, 100nM)produced no significant changes in the 5-HT contractile response (all p=NSD). 5-HTproduced concentration-dependent contractions in porcine urothelium and lamina propria andthis response was unlikely to be mediated by receptors 5-HT2, 5-HT3 or 5-HT4. Preliminaryresponses appear to indicate involvement of 5-HT1 receptors. This study shows 5-HTreceptors may be a viable option for bladder dysfunction research and treatment.

M3 - Abstract

ER -

Edwards EJ, Chess-Williams R, Moro C. The contractile effects of 5-hydroxytryptamine on porcine bladder urothelium and lamina propria. 2015. Abstract from The7th National Symposium on Advances in Gastrointestinal & Urogenital Research , Adelaide, Australia.