Temperature-dependent calcium-induced calcium release via InsP3 receptors in mouse olfactory ensheathing glial cells

Maren Stavermann, Kristina Buddrus, James A. St John, Jenny A K Ekberg, Bernd Nilius, Joachim W. Deitmer, Christian Lohr

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13 Citations (Scopus)

Abstract

Cooling can induce Ca2+ signaling via activation of temperature-sensitive ion channels such as TRPM8, TRPA1 and ryanodine receptor channels. Here we have studied the mechanism of cooling-evoked Ca2+ signaling in mouse olfactory ensheathing cells (OECs), a specialized type of glial cells in the olfactory nerve layer of the olfactory bulb. Reducing the temperature from above 30°C to 28°C and below triggered Ca2+ transients that persisted in the absence of external Ca2+, but were suppressed after Ca2+ store depletion by cyclopiazonic acid. Cooling-evoked Ca2+ transients were present in mice deficient of TRPM8 and TRPA1, and were not inhibited by ryanodine receptor antagonists. Inhibition of InsP3 receptors with 2-APB and caffeine entirely blocked cooling-evoked Ca2+ transients. Moderate Ca2+ increases, as evoked by flash photolysis of NP-EGTA (caged Ca2+) and cyclopiazonic acid, triggered InsP3 receptor-mediated Ca2+ release at 22°C, but not at 31°C. The results suggest that InsP3 receptors mediate Ca2+-induced Ca2+ release in OECs, and that this Ca2+ release is temperature-sensitive and can be suppressed at temperatures above 28°C.

Original languageEnglish
Pages (from-to)113-123
Number of pages11
JournalCell Calcium
Volume52
Issue number2
DOIs
Publication statusPublished - Aug 2012
Externally publishedYes

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Neuroglia
Calcium
Ryanodine Receptor Calcium Release Channel
Temperature
Olfactory Nerve
Photolysis
Olfactory Bulb
Caffeine
Ion Channels
cyclopiazonic acid

Cite this

Stavermann, M., Buddrus, K., St John, J. A., Ekberg, J. A. K., Nilius, B., Deitmer, J. W., & Lohr, C. (2012). Temperature-dependent calcium-induced calcium release via InsP3 receptors in mouse olfactory ensheathing glial cells. Cell Calcium, 52(2), 113-123. https://doi.org/10.1016/j.ceca.2012.04.017
Stavermann, Maren ; Buddrus, Kristina ; St John, James A. ; Ekberg, Jenny A K ; Nilius, Bernd ; Deitmer, Joachim W. ; Lohr, Christian. / Temperature-dependent calcium-induced calcium release via InsP3 receptors in mouse olfactory ensheathing glial cells. In: Cell Calcium. 2012 ; Vol. 52, No. 2. pp. 113-123.
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abstract = "Cooling can induce Ca2+ signaling via activation of temperature-sensitive ion channels such as TRPM8, TRPA1 and ryanodine receptor channels. Here we have studied the mechanism of cooling-evoked Ca2+ signaling in mouse olfactory ensheathing cells (OECs), a specialized type of glial cells in the olfactory nerve layer of the olfactory bulb. Reducing the temperature from above 30°C to 28°C and below triggered Ca2+ transients that persisted in the absence of external Ca2+, but were suppressed after Ca2+ store depletion by cyclopiazonic acid. Cooling-evoked Ca2+ transients were present in mice deficient of TRPM8 and TRPA1, and were not inhibited by ryanodine receptor antagonists. Inhibition of InsP3 receptors with 2-APB and caffeine entirely blocked cooling-evoked Ca2+ transients. Moderate Ca2+ increases, as evoked by flash photolysis of NP-EGTA (caged Ca2+) and cyclopiazonic acid, triggered InsP3 receptor-mediated Ca2+ release at 22°C, but not at 31°C. The results suggest that InsP3 receptors mediate Ca2+-induced Ca2+ release in OECs, and that this Ca2+ release is temperature-sensitive and can be suppressed at temperatures above 28°C.",
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Stavermann, M, Buddrus, K, St John, JA, Ekberg, JAK, Nilius, B, Deitmer, JW & Lohr, C 2012, 'Temperature-dependent calcium-induced calcium release via InsP3 receptors in mouse olfactory ensheathing glial cells' Cell Calcium, vol. 52, no. 2, pp. 113-123. https://doi.org/10.1016/j.ceca.2012.04.017

Temperature-dependent calcium-induced calcium release via InsP3 receptors in mouse olfactory ensheathing glial cells. / Stavermann, Maren; Buddrus, Kristina; St John, James A.; Ekberg, Jenny A K; Nilius, Bernd; Deitmer, Joachim W.; Lohr, Christian.

In: Cell Calcium, Vol. 52, No. 2, 08.2012, p. 113-123.

Research output: Contribution to journalArticleResearchpeer-review

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