Stromal cell subsets directing neonatal spleen regeneration

Jonathan K H Tan, Takeshi Watanabe

Research output: Contribution to journalArticleResearchpeer-review

8 Citations (Scopus)
122 Downloads (Pure)

Abstract

Development of lymphoid tissue is determined by interactions between stromal lymphoid tissue organiser (LTo) and hematopoietic lymphoid tissue inducer (LTi) cells. A failure for LTo to receive appropriate activating signals during embryogenesis through lymphotoxin engagement leads to a complete cessation of lymph node (LN) and Peyer's patch development, identifying LTo as a key stromal population for lymphoid tissue organogenesis. However, little is known about the equivalent stromal cells that induce spleen development. Here, by dissociating neonatal murine spleen stromal tissue for re-aggregation and transplant into adult mouse recipients, we have identified a MAdCAM-1 + CD31 + CD201 + spleen stromal organizer cell-type critical for new tissue formation. This finding provides an insight into the regulation of post-natal spleen tissue organogenesis, and could be exploited in the development of spleen regenerative therapies.

Original languageEnglish
Article number40401
JournalScientific Reports
Volume7
DOIs
Publication statusPublished - 9 Jan 2017

Fingerprint

Lymphoid Tissue
Stromal Cells
Regeneration
Spleen
Organogenesis
Lymphotoxin-alpha
Peyer's Patches
Helper-Inducer T-Lymphocytes
Embryonic Development
Lymph Nodes
Transplants
Population

Cite this

@article{83e560b435d34eed85467720c7a76174,
title = "Stromal cell subsets directing neonatal spleen regeneration",
abstract = "Development of lymphoid tissue is determined by interactions between stromal lymphoid tissue organiser (LTo) and hematopoietic lymphoid tissue inducer (LTi) cells. A failure for LTo to receive appropriate activating signals during embryogenesis through lymphotoxin engagement leads to a complete cessation of lymph node (LN) and Peyer's patch development, identifying LTo as a key stromal population for lymphoid tissue organogenesis. However, little is known about the equivalent stromal cells that induce spleen development. Here, by dissociating neonatal murine spleen stromal tissue for re-aggregation and transplant into adult mouse recipients, we have identified a MAdCAM-1 + CD31 + CD201 + spleen stromal organizer cell-type critical for new tissue formation. This finding provides an insight into the regulation of post-natal spleen tissue organogenesis, and could be exploited in the development of spleen regenerative therapies.",
author = "Tan, {Jonathan K H} and Takeshi Watanabe",
year = "2017",
month = "1",
day = "9",
doi = "10.1038/srep40401",
language = "English",
volume = "7",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",

}

Stromal cell subsets directing neonatal spleen regeneration. / Tan, Jonathan K H; Watanabe, Takeshi.

In: Scientific Reports, Vol. 7, 40401, 09.01.2017.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Stromal cell subsets directing neonatal spleen regeneration

AU - Tan, Jonathan K H

AU - Watanabe, Takeshi

PY - 2017/1/9

Y1 - 2017/1/9

N2 - Development of lymphoid tissue is determined by interactions between stromal lymphoid tissue organiser (LTo) and hematopoietic lymphoid tissue inducer (LTi) cells. A failure for LTo to receive appropriate activating signals during embryogenesis through lymphotoxin engagement leads to a complete cessation of lymph node (LN) and Peyer's patch development, identifying LTo as a key stromal population for lymphoid tissue organogenesis. However, little is known about the equivalent stromal cells that induce spleen development. Here, by dissociating neonatal murine spleen stromal tissue for re-aggregation and transplant into adult mouse recipients, we have identified a MAdCAM-1 + CD31 + CD201 + spleen stromal organizer cell-type critical for new tissue formation. This finding provides an insight into the regulation of post-natal spleen tissue organogenesis, and could be exploited in the development of spleen regenerative therapies.

AB - Development of lymphoid tissue is determined by interactions between stromal lymphoid tissue organiser (LTo) and hematopoietic lymphoid tissue inducer (LTi) cells. A failure for LTo to receive appropriate activating signals during embryogenesis through lymphotoxin engagement leads to a complete cessation of lymph node (LN) and Peyer's patch development, identifying LTo as a key stromal population for lymphoid tissue organogenesis. However, little is known about the equivalent stromal cells that induce spleen development. Here, by dissociating neonatal murine spleen stromal tissue for re-aggregation and transplant into adult mouse recipients, we have identified a MAdCAM-1 + CD31 + CD201 + spleen stromal organizer cell-type critical for new tissue formation. This finding provides an insight into the regulation of post-natal spleen tissue organogenesis, and could be exploited in the development of spleen regenerative therapies.

UR - http://www.scopus.com/inward/record.url?scp=85008967572&partnerID=8YFLogxK

U2 - 10.1038/srep40401

DO - 10.1038/srep40401

M3 - Article

VL - 7

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

M1 - 40401

ER -