@inbook{e94c8109dfdb4acb927f8c0f76fd8e79,
title = "Steroid-Nitroxide Hybrid Compound Protects the Retina in a Model of CNV",
abstract = "Nitroxide-based drugs have proven effective in modulating radical-induced oxidative stress by modulating antioxidant enzymes and genes that control distinct immune and anti-inflammatory responses. Due to their reasonable chemical stability and ability to shuttle between oxidized and reduced forms at physiologically relevant redox potentials, nitroxide-based radicals have also proven effective as biological probes of redox status. Herein, we investigated the potential of a unique nitroxide-based antioxidant and anti-inflammatory agent to protect the retina from experimentally induced degeneration. An established rat model of retinal degeneration was used viz. laser-induced choroidal neovascularization (CNV) to study the effects of the hybrid steroidal anti-inflammatory-antioxidant prednisolone 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO) nitroxide compound. Vascular endothelial cell distribution at the CNV lesion site was investigated using isolectin B4 fluorescence histology, and the inflammatory response of microglia was investigated using IBA-1 immunohistochemistry. The prednisolone-TEMPO (Pred-TEMPO) hybrid reduced the laser-induced CNV lesion area compared to untreated control rats. These findings demonstrate that nitroxide-based compounds are potential therapeutics for retinal degenerative diseases involving inflammatory and oxidative stress-mediated components, including age-related macular degeneration.",
author = "Rimaz Toto and Soltau, \{Carl P.\} and Rayner, \{Cassie L.\} and Bottle, \{Steven E.\} and Barnett, \{Nigel L.\}",
year = "2025",
month = feb,
day = "11",
doi = "10.1007/978-3-031-76550-6\_81",
language = "English",
isbn = "9783031765490",
volume = "1468",
series = "Advances in Experimental Medicine and Biology",
publisher = "Springer",
pages = "497--501",
booktitle = "Retinol Degenerative Diseases XX",
address = "Germany",
}