Spontaneous activity in isolated bladder strips from the streptozotocin-induced diabetic rat: Effect of cholinergic modulation and the mucosa

Donna J Sellers; Neil G. McKay; Kim Lawson; Bahareh Vahabi

Spontaneous activity in isolated bladder strips from the streptozotocin-induced diabetic rat: Effect of cholinergic modulation and the mucosa

Donna J Sellers
, Neil G. McKay
, Kim Lawson
, Bahareh Vahabi

Centre for Urology Research

Research output: Contribution to conference › Abstract › Research › peer-review

Abstract

Introduction. In bladder overactivity increased spontaneous contractions (SCs) may underlie abnormal increases in intravesical pressure, leading to urgency, frequency and incontinence. SCs are also increased in models of bladder dysfunction, including the diabetic rat (Stevens et al., 2006; Vahabi et al. 2007), possibly due to leakage of acetylcholine from intramural nerves (Ng et al. 2006) or the mucosa.
Aim. To study cholinergic modulation and the role of the mucosa on SCs in bladder strips from diabetic rats.
Methods. Intact and mucosa-denuded bladder strips, from male Wistar rats, 1- and 12-weeks following streptozotocin administration (65mg/kg, i.p.) or age-matched controls, were mounted in Krebs-bicarbonate solution (2g tension, 37°C). SCs were induced by carbachol (CCH) (0.05-0.5ìM), and amplitude and frequency of SCs measured.

Original language	English
Pages	13-13
Number of pages	1
Publication status	Published - 2010
Event	ASCEPT 44th Annual Scientific Meeting 2010 : Found in Translation: Integrated Approaches to Drug Development - Melbourne , Australia Duration: 28 Nov 2010 → 1 Dec 2010 http://ascept.eproceedings.com.au/

Conference

Conference	ASCEPT 44th Annual Scientific Meeting 2010
Abbreviated title	ASCEPT
Country/Territory	Australia
City	Melbourne
Period	28/11/10 → 1/12/10
Internet address	http://ascept.eproceedings.com.au/

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This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

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Cite this

@conference{0c0ea64a8fc44e34889fa57097c72540,

title = "Spontaneous activity in isolated bladder strips from the streptozotocin-induced diabetic rat: Effect of cholinergic modulation and the mucosa",

abstract = "Introduction. In bladder overactivity increased spontaneous contractions (SCs) may underlie abnormal increases in intravesical pressure, leading to urgency, frequency and incontinence. SCs are also increased in models of bladder dysfunction, including the diabetic rat (Stevens et al., 2006; Vahabi et al. 2007), possibly due to leakage of acetylcholine from intramural nerves (Ng et al. 2006) or the mucosa. Aim. To study cholinergic modulation and the role of the mucosa on SCs in bladder strips from diabetic rats. Methods. Intact and mucosa-denuded bladder strips, from male Wistar rats, 1- and 12-weeks following streptozotocin administration (65mg/kg, i.p.) or age-matched controls, were mounted in Krebs-bicarbonate solution (2g tension, 37°C). SCs were induced by carbachol (CCH) (0.05-0.5{\`i}M), and amplitude and frequency of SCs measured.",

author = "Sellers, \{Donna J\} and McKay, \{Neil G.\} and Kim Lawson and Bahareh Vahabi",

year = "2010",

language = "English",

pages = "13--13",

note = "ASCEPT 44th Annual Scientific Meeting 2010 : Found in Translation: Integrated Approaches to Drug Development, ASCEPT ; Conference date: 28-11-2010 Through 01-12-2010",

url = "http://ascept.eproceedings.com.au/",

}

Spontaneous activity in isolated bladder strips from the streptozotocin-induced diabetic rat: Effect of cholinergic modulation and the mucosa. / Sellers, Donna J; McKay, Neil G.; Lawson, Kim et al.
2010. 13-13 Abstract from ASCEPT 44th Annual Scientific Meeting 2010 , Melbourne , Australia.

Research output: Contribution to conference › Abstract › Research › peer-review

TY - CONF

T1 - Spontaneous activity in isolated bladder strips from the streptozotocin-induced diabetic rat

T2 - ASCEPT 44th Annual Scientific Meeting 2010

AU - Sellers, Donna J

AU - McKay, Neil G.

AU - Lawson, Kim

AU - Vahabi, Bahareh

PY - 2010

Y1 - 2010

N2 - Introduction. In bladder overactivity increased spontaneous contractions (SCs) may underlie abnormal increases in intravesical pressure, leading to urgency, frequency and incontinence. SCs are also increased in models of bladder dysfunction, including the diabetic rat (Stevens et al., 2006; Vahabi et al. 2007), possibly due to leakage of acetylcholine from intramural nerves (Ng et al. 2006) or the mucosa. Aim. To study cholinergic modulation and the role of the mucosa on SCs in bladder strips from diabetic rats. Methods. Intact and mucosa-denuded bladder strips, from male Wistar rats, 1- and 12-weeks following streptozotocin administration (65mg/kg, i.p.) or age-matched controls, were mounted in Krebs-bicarbonate solution (2g tension, 37°C). SCs were induced by carbachol (CCH) (0.05-0.5ìM), and amplitude and frequency of SCs measured.

AB - Introduction. In bladder overactivity increased spontaneous contractions (SCs) may underlie abnormal increases in intravesical pressure, leading to urgency, frequency and incontinence. SCs are also increased in models of bladder dysfunction, including the diabetic rat (Stevens et al., 2006; Vahabi et al. 2007), possibly due to leakage of acetylcholine from intramural nerves (Ng et al. 2006) or the mucosa. Aim. To study cholinergic modulation and the role of the mucosa on SCs in bladder strips from diabetic rats. Methods. Intact and mucosa-denuded bladder strips, from male Wistar rats, 1- and 12-weeks following streptozotocin administration (65mg/kg, i.p.) or age-matched controls, were mounted in Krebs-bicarbonate solution (2g tension, 37°C). SCs were induced by carbachol (CCH) (0.05-0.5ìM), and amplitude and frequency of SCs measured.

M3 - Abstract

SP - 13

EP - 13

Y2 - 28 November 2010 through 1 December 2010

ER -

Spontaneous activity in isolated bladder strips from the streptozotocin-induced diabetic rat: Effect of cholinergic modulation and the mucosa

Abstract

Conference

UN SDGs

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