Spontaneous activity in isolated bladder strips from the streptozotocin-induced diabetic rat: Effect of cholinergic modulation and the mucosa

Donna J Sellers, Neil G. McKay, Kim Lawson, Bahareh Vahabi

Research output: Contribution to conferenceAbstractResearchpeer-review

Abstract

Introduction. In bladder overactivity increased spontaneous contractions (SCs) may underlie abnormal increases in intravesical pressure, leading to urgency, frequency and incontinence. SCs are also increased in models of bladder dysfunction, including the diabetic rat (Stevens et al., 2006; Vahabi et al. 2007), possibly due to leakage of acetylcholine from intramural nerves (Ng et al. 2006) or the mucosa.
Aim. To study cholinergic modulation and the role of the mucosa on SCs in bladder strips from diabetic rats.
Methods. Intact and mucosa-denuded bladder strips, from male Wistar rats, 1- and 12-weeks following streptozotocin administration (65mg/kg, i.p.) or age-matched controls, were mounted in Krebs-bicarbonate solution (2g tension, 37°C). SCs were induced by carbachol (CCH) (0.05-0.5ìM), and amplitude and frequency of SCs measured.
Original languageEnglish
Pages13-13
Number of pages1
Publication statusPublished - 2010
EventASCEPT 44th Annual Scientific Meeting 2010 : Found in Translation: Integrated Approaches to Drug Development - Melbourne , Australia
Duration: 28 Nov 20101 Dec 2010
http://ascept.eproceedings.com.au/

Conference

ConferenceASCEPT 44th Annual Scientific Meeting 2010
Abbreviated titleASCEPT
CountryAustralia
CityMelbourne
Period28/11/101/12/10
Internet address

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Streptozocin
Cholinergic Agents
Mucous Membrane
Urinary Bladder
Carbachol
Bicarbonates
Acetylcholine
Wistar Rats
Pressure

Cite this

Sellers, D. J., McKay, N. G., Lawson, K., & Vahabi, B. (2010). Spontaneous activity in isolated bladder strips from the streptozotocin-induced diabetic rat: Effect of cholinergic modulation and the mucosa. 13-13. Abstract from ASCEPT 44th Annual Scientific Meeting 2010 , Melbourne , Australia.
Sellers, Donna J ; McKay, Neil G. ; Lawson, Kim ; Vahabi, Bahareh. / Spontaneous activity in isolated bladder strips from the streptozotocin-induced diabetic rat : Effect of cholinergic modulation and the mucosa. Abstract from ASCEPT 44th Annual Scientific Meeting 2010 , Melbourne , Australia.1 p.
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title = "Spontaneous activity in isolated bladder strips from the streptozotocin-induced diabetic rat: Effect of cholinergic modulation and the mucosa",
abstract = "Introduction. In bladder overactivity increased spontaneous contractions (SCs) may underlie abnormal increases in intravesical pressure, leading to urgency, frequency and incontinence. SCs are also increased in models of bladder dysfunction, including the diabetic rat (Stevens et al., 2006; Vahabi et al. 2007), possibly due to leakage of acetylcholine from intramural nerves (Ng et al. 2006) or the mucosa.Aim. To study cholinergic modulation and the role of the mucosa on SCs in bladder strips from diabetic rats.Methods. Intact and mucosa-denuded bladder strips, from male Wistar rats, 1- and 12-weeks following streptozotocin administration (65mg/kg, i.p.) or age-matched controls, were mounted in Krebs-bicarbonate solution (2g tension, 37°C). SCs were induced by carbachol (CCH) (0.05-0.5{\`i}M), and amplitude and frequency of SCs measured.",
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Sellers, DJ, McKay, NG, Lawson, K & Vahabi, B 2010, 'Spontaneous activity in isolated bladder strips from the streptozotocin-induced diabetic rat: Effect of cholinergic modulation and the mucosa' ASCEPT 44th Annual Scientific Meeting 2010 , Melbourne , Australia, 28/11/10 - 1/12/10, pp. 13-13.

Spontaneous activity in isolated bladder strips from the streptozotocin-induced diabetic rat : Effect of cholinergic modulation and the mucosa. / Sellers, Donna J; McKay, Neil G.; Lawson, Kim; Vahabi, Bahareh.

2010. 13-13 Abstract from ASCEPT 44th Annual Scientific Meeting 2010 , Melbourne , Australia.

Research output: Contribution to conferenceAbstractResearchpeer-review

TY - CONF

T1 - Spontaneous activity in isolated bladder strips from the streptozotocin-induced diabetic rat

T2 - Effect of cholinergic modulation and the mucosa

AU - Sellers, Donna J

AU - McKay, Neil G.

AU - Lawson, Kim

AU - Vahabi, Bahareh

PY - 2010

Y1 - 2010

N2 - Introduction. In bladder overactivity increased spontaneous contractions (SCs) may underlie abnormal increases in intravesical pressure, leading to urgency, frequency and incontinence. SCs are also increased in models of bladder dysfunction, including the diabetic rat (Stevens et al., 2006; Vahabi et al. 2007), possibly due to leakage of acetylcholine from intramural nerves (Ng et al. 2006) or the mucosa.Aim. To study cholinergic modulation and the role of the mucosa on SCs in bladder strips from diabetic rats.Methods. Intact and mucosa-denuded bladder strips, from male Wistar rats, 1- and 12-weeks following streptozotocin administration (65mg/kg, i.p.) or age-matched controls, were mounted in Krebs-bicarbonate solution (2g tension, 37°C). SCs were induced by carbachol (CCH) (0.05-0.5ìM), and amplitude and frequency of SCs measured.

AB - Introduction. In bladder overactivity increased spontaneous contractions (SCs) may underlie abnormal increases in intravesical pressure, leading to urgency, frequency and incontinence. SCs are also increased in models of bladder dysfunction, including the diabetic rat (Stevens et al., 2006; Vahabi et al. 2007), possibly due to leakage of acetylcholine from intramural nerves (Ng et al. 2006) or the mucosa.Aim. To study cholinergic modulation and the role of the mucosa on SCs in bladder strips from diabetic rats.Methods. Intact and mucosa-denuded bladder strips, from male Wistar rats, 1- and 12-weeks following streptozotocin administration (65mg/kg, i.p.) or age-matched controls, were mounted in Krebs-bicarbonate solution (2g tension, 37°C). SCs were induced by carbachol (CCH) (0.05-0.5ìM), and amplitude and frequency of SCs measured.

M3 - Abstract

SP - 13

EP - 13

ER -

Sellers DJ, McKay NG, Lawson K, Vahabi B. Spontaneous activity in isolated bladder strips from the streptozotocin-induced diabetic rat: Effect of cholinergic modulation and the mucosa. 2010. Abstract from ASCEPT 44th Annual Scientific Meeting 2010 , Melbourne , Australia.