Spontaneous activity in isolated bladder strips from the streptozotocin-induced diabetic rat: Effect of cholinergic modulation and the mucosa

Donna J Sellers, Neil G. McKay, Kim Lawson, Bahareh Vahabi

Research output: Contribution to conferenceAbstractResearchpeer-review

Abstract

Introduction. In bladder overactivity increased spontaneous contractions (SCs) may underlie abnormal increases in intravesical pressure, leading to urgency, frequency and incontinence. SCs are also increased in models of bladder dysfunction, including the diabetic rat (Stevens et al., 2006; Vahabi et al. 2007), possibly due to leakage of acetylcholine from intramural nerves (Ng et al. 2006) or the mucosa.
Aim. To study cholinergic modulation and the role of the mucosa on SCs in bladder strips from diabetic rats.
Methods. Intact and mucosa-denuded bladder strips, from male Wistar rats, 1- and 12-weeks following streptozotocin administration (65mg/kg, i.p.) or age-matched controls, were mounted in Krebs-bicarbonate solution (2g tension, 37°C). SCs were induced by carbachol (CCH) (0.05-0.5ìM), and amplitude and frequency of SCs measured.
Original languageEnglish
Pages13-13
Number of pages1
Publication statusPublished - 2010
EventASCEPT 44th Annual Scientific Meeting 2010 : Found in Translation: Integrated Approaches to Drug Development - Melbourne , Australia
Duration: 28 Nov 20101 Dec 2010
http://ascept.eproceedings.com.au/

Conference

ConferenceASCEPT 44th Annual Scientific Meeting 2010
Abbreviated titleASCEPT
CountryAustralia
CityMelbourne
Period28/11/101/12/10
Internet address

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Sellers, D. J., McKay, N. G., Lawson, K., & Vahabi, B. (2010). Spontaneous activity in isolated bladder strips from the streptozotocin-induced diabetic rat: Effect of cholinergic modulation and the mucosa. 13-13. Abstract from ASCEPT 44th Annual Scientific Meeting 2010 , Melbourne , Australia.