Splenic stromal niches support hematopoiesis of dendritic-like cells from precursors in bone marrow and spleen

Pravin Periasamy, Jonathan K. H. Tan, Kristin L. Griffiths, Helen C. O'Neill

Research output: Contribution to journalArticleResearchpeer-review

38 Citations (Scopus)

Abstract

Objective. The aims of this study are to test the ability of stromal cells from murine spleen to support hematopoiesis, to define the tissue source of precursors that seed these hematopoietic niches, and to determine the type of cells produced.

Materials and Methods. Cloned isolates of murine spleen stroma have been developed that support hematopoiesis. Analysis has been investigated in terms of tissue source of progenitors. Type and number of cells produced were analyzed by How cytometry.

Results. Hematopoietic precursors that seed cocultures exist in spleen and bone marrow (BM), but not thymus. Cell production is highest if overlay cells are enriched for hematopoietic precursors. BM contains more precursors than spleen, but the cell types produced are different. Cocultures established from spleen maintain a high proportion of a distinct class of dendritic-like cells produced in only low numbers in BM cocultures. These reflect the immature myeloid dendritic cell (DC) produced continuously in long-term spleen cultures established previously in this laboratory. Stroma-conditioned medium alone does not support DC development, but does support early outgrowth of myelomonocytic cells from precursors in both spleen and BM.

Conclusion. The outcome has been development of a coculture system that supports hematopoiesis of immature myeloid dendritic-like cells in vitro. Although production of monocytes can occur in the presence of stroma-conditioned medium alone, production of DC is dependent on stromal cell interaction. Results presented here raise questions about the role of spleen as a site for DC hematopoiesis from endogenous precursors. (C) 2009 ISEH - Society for Hematology and Stem Cells. Published by Elsevier Inc.

Original languageEnglish
Pages (from-to)1060-1071
Number of pages12
JournalExperimental Hematology
Volume37
Issue number9
DOIs
Publication statusPublished - Sep 2009
Externally publishedYes

Cite this

@article{f86f695560d14ffba6a89af094f631b4,
title = "Splenic stromal niches support hematopoiesis of dendritic-like cells from precursors in bone marrow and spleen",
abstract = "Objective. The aims of this study are to test the ability of stromal cells from murine spleen to support hematopoiesis, to define the tissue source of precursors that seed these hematopoietic niches, and to determine the type of cells produced.Materials and Methods. Cloned isolates of murine spleen stroma have been developed that support hematopoiesis. Analysis has been investigated in terms of tissue source of progenitors. Type and number of cells produced were analyzed by How cytometry.Results. Hematopoietic precursors that seed cocultures exist in spleen and bone marrow (BM), but not thymus. Cell production is highest if overlay cells are enriched for hematopoietic precursors. BM contains more precursors than spleen, but the cell types produced are different. Cocultures established from spleen maintain a high proportion of a distinct class of dendritic-like cells produced in only low numbers in BM cocultures. These reflect the immature myeloid dendritic cell (DC) produced continuously in long-term spleen cultures established previously in this laboratory. Stroma-conditioned medium alone does not support DC development, but does support early outgrowth of myelomonocytic cells from precursors in both spleen and BM.Conclusion. The outcome has been development of a coculture system that supports hematopoiesis of immature myeloid dendritic-like cells in vitro. Although production of monocytes can occur in the presence of stroma-conditioned medium alone, production of DC is dependent on stromal cell interaction. Results presented here raise questions about the role of spleen as a site for DC hematopoiesis from endogenous precursors. (C) 2009 ISEH - Society for Hematology and Stem Cells. Published by Elsevier Inc.",
author = "Pravin Periasamy and Tan, {Jonathan K. H.} and Griffiths, {Kristin L.} and O'Neill, {Helen C.}",
year = "2009",
month = "9",
doi = "10.1016/j.exphem.2009.06.001",
language = "English",
volume = "37",
pages = "1060--1071",
journal = "Experimental Hematology",
issn = "0301-472X",
publisher = "Elsevier",
number = "9",

}

Splenic stromal niches support hematopoiesis of dendritic-like cells from precursors in bone marrow and spleen. / Periasamy, Pravin; Tan, Jonathan K. H.; Griffiths, Kristin L.; O'Neill, Helen C.

In: Experimental Hematology, Vol. 37, No. 9, 09.2009, p. 1060-1071.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Splenic stromal niches support hematopoiesis of dendritic-like cells from precursors in bone marrow and spleen

AU - Periasamy, Pravin

AU - Tan, Jonathan K. H.

AU - Griffiths, Kristin L.

AU - O'Neill, Helen C.

PY - 2009/9

Y1 - 2009/9

N2 - Objective. The aims of this study are to test the ability of stromal cells from murine spleen to support hematopoiesis, to define the tissue source of precursors that seed these hematopoietic niches, and to determine the type of cells produced.Materials and Methods. Cloned isolates of murine spleen stroma have been developed that support hematopoiesis. Analysis has been investigated in terms of tissue source of progenitors. Type and number of cells produced were analyzed by How cytometry.Results. Hematopoietic precursors that seed cocultures exist in spleen and bone marrow (BM), but not thymus. Cell production is highest if overlay cells are enriched for hematopoietic precursors. BM contains more precursors than spleen, but the cell types produced are different. Cocultures established from spleen maintain a high proportion of a distinct class of dendritic-like cells produced in only low numbers in BM cocultures. These reflect the immature myeloid dendritic cell (DC) produced continuously in long-term spleen cultures established previously in this laboratory. Stroma-conditioned medium alone does not support DC development, but does support early outgrowth of myelomonocytic cells from precursors in both spleen and BM.Conclusion. The outcome has been development of a coculture system that supports hematopoiesis of immature myeloid dendritic-like cells in vitro. Although production of monocytes can occur in the presence of stroma-conditioned medium alone, production of DC is dependent on stromal cell interaction. Results presented here raise questions about the role of spleen as a site for DC hematopoiesis from endogenous precursors. (C) 2009 ISEH - Society for Hematology and Stem Cells. Published by Elsevier Inc.

AB - Objective. The aims of this study are to test the ability of stromal cells from murine spleen to support hematopoiesis, to define the tissue source of precursors that seed these hematopoietic niches, and to determine the type of cells produced.Materials and Methods. Cloned isolates of murine spleen stroma have been developed that support hematopoiesis. Analysis has been investigated in terms of tissue source of progenitors. Type and number of cells produced were analyzed by How cytometry.Results. Hematopoietic precursors that seed cocultures exist in spleen and bone marrow (BM), but not thymus. Cell production is highest if overlay cells are enriched for hematopoietic precursors. BM contains more precursors than spleen, but the cell types produced are different. Cocultures established from spleen maintain a high proportion of a distinct class of dendritic-like cells produced in only low numbers in BM cocultures. These reflect the immature myeloid dendritic cell (DC) produced continuously in long-term spleen cultures established previously in this laboratory. Stroma-conditioned medium alone does not support DC development, but does support early outgrowth of myelomonocytic cells from precursors in both spleen and BM.Conclusion. The outcome has been development of a coculture system that supports hematopoiesis of immature myeloid dendritic-like cells in vitro. Although production of monocytes can occur in the presence of stroma-conditioned medium alone, production of DC is dependent on stromal cell interaction. Results presented here raise questions about the role of spleen as a site for DC hematopoiesis from endogenous precursors. (C) 2009 ISEH - Society for Hematology and Stem Cells. Published by Elsevier Inc.

U2 - 10.1016/j.exphem.2009.06.001

DO - 10.1016/j.exphem.2009.06.001

M3 - Article

VL - 37

SP - 1060

EP - 1071

JO - Experimental Hematology

JF - Experimental Hematology

SN - 0301-472X

IS - 9

ER -