Hematopoiesis occurs throughout the lifespan of an organism. This process involves the formation of blood cells from self-renewing hematopoietic stem cell (HSC). The hematopoietic niche environment which comprises non-hematopoietic stromal cells regulates the quiescence, dormancy, self-renewal and differentiation of HSC. It is known that multiple HSC niches including endosteal, vascular and perivascular exist in the bone marrow, although less is known for other sites like spleen. Previous studies in this lab have described unique splenic stroma cell lines 5G3 and 3B5 which can support hematopoiesis and reflect HSC niches. Transcriptome data previously obtained have also shown that 5G3 and 3B5 express many genes in parallel with perivascular cells described in bone marrow. The study here shows that both 5G3 and 3B5 stroma share a mesenchymal lineage origin with perivascular cells in bone marrow, including mesenchymal stem cells and C-XC motif ligand 12 (CXCL12)-abundant reticular cells. 5G3 and 3B5 express many cell surface markers in common with these cells including CD105, CD29, VCAM1, Sca-1, CD51, CD140a and Thy1.2. In addition, splenic stromal cells with the phenotype of Sca-1+gp38+Thy1.2+CD29+CD51+, reflecting cells of mesenchymal lineage were found to be important for in vitro hematopoiesis. Stromal cells expressing gp38 or Thy1.2 appear to be associated with some HSC in spleen identified through section staining. Restricted hematopoiesis giving rise to L-DC and myeloid cells was replicated in vivo following grafting of splenic stromal cell lines including 5G3, 10C9 and 3B5 under the kidney capsule. Inhibition assays were also used to identify the important role of SCF and Notch but not CXCL12 in supporting in vitro hematopoiesis. This study has improved our current understanding of HSC niches in spleen and identified some of the molecular regulators of hematopoiesis. Information obtained will be important for development therapies involving splenic or ectopic niche to enhance hematopoietic output, in immunocompromised patients or following HSC transplantation.
|Published - 22 Aug 2018
|47th Annual Scientific Meeting of the ISEH - International Society for Experimental Hematology - Luskin Conference Center, Los Angeles, United States
Duration: 23 Aug 2018 → 26 Aug 2018
Conference number: 47th