Simultaneous monitoring of vascular contractility, intracellular pH and intracellular calcium in isolated rat mesenteric arteries; effects of weak bases

Donna Otter; Clare Austin

doi:10.1111/j.1469-445X.2000.00353.x

Simultaneous monitoring of vascular contractility, intracellular pH and intracellular calcium in isolated rat mesenteric arteries; effects of weak bases

Donna Otter
, Clare Austin^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Research › peer-review

15 Citations (Scopus)

Abstract

We report the first simultaneous measurements of pH(i), [Ca²⁺](i) and tension, upon alteration of pH(i), in isolated rat mesenteric arteries loaded with both carboxy SNARF and indo-1. In these vessels (pre-contracted by 30 mM KCI) alterations of pH(i), by addition and subsequent washout of weak bases, produced complicated effects on tone. Although the changes in contractility did not mirror the changes in pH(i) they were, at all times, accompanied by parallel changes in [Ca²⁺](i).

Original language	English
Pages (from-to)	349-351
Number of pages	3
Journal	Experimental Physiology
Volume	85
Issue number	3
DOIs	https://doi.org/10.1111/j.1469-445X.2000.00353.x
Publication status	Published - May 2000
Externally published	Yes

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abstract = "We report the first simultaneous measurements of pH(i), [Ca2+](i) and tension, upon alteration of pH(i), in isolated rat mesenteric arteries loaded with both carboxy SNARF and indo-1. In these vessels (pre-contracted by 30 mM KCI) alterations of pH(i), by addition and subsequent washout of weak bases, produced complicated effects on tone. Although the changes in contractility did not mirror the changes in pH(i) they were, at all times, accompanied by parallel changes in [Ca2+](i).",

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AB - We report the first simultaneous measurements of pH(i), [Ca2+](i) and tension, upon alteration of pH(i), in isolated rat mesenteric arteries loaded with both carboxy SNARF and indo-1. In these vessels (pre-contracted by 30 mM KCI) alterations of pH(i), by addition and subsequent washout of weak bases, produced complicated effects on tone. Although the changes in contractility did not mirror the changes in pH(i) they were, at all times, accompanied by parallel changes in [Ca2+](i).

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Simultaneous monitoring of vascular contractility, intracellular pH and intracellular calcium in isolated rat mesenteric arteries; effects of weak bases

Abstract

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