TY - JOUR
T1 - Simultaneous monitoring of vascular contractility, intracellular pH and intracellular calcium in isolated rat mesenteric arteries; effects of weak bases
AU - Otter, Donna
AU - Austin, Clare
PY - 2000/5
Y1 - 2000/5
N2 - We report the first simultaneous measurements of pH(i), [Ca2+](i) and tension, upon alteration of pH(i), in isolated rat mesenteric arteries loaded with both carboxy SNARF and indo-1. In these vessels (pre-contracted by 30 mM KCI) alterations of pH(i), by addition and subsequent washout of weak bases, produced complicated effects on tone. Although the changes in contractility did not mirror the changes in pH(i) they were, at all times, accompanied by parallel changes in [Ca2+](i).
AB - We report the first simultaneous measurements of pH(i), [Ca2+](i) and tension, upon alteration of pH(i), in isolated rat mesenteric arteries loaded with both carboxy SNARF and indo-1. In these vessels (pre-contracted by 30 mM KCI) alterations of pH(i), by addition and subsequent washout of weak bases, produced complicated effects on tone. Although the changes in contractility did not mirror the changes in pH(i) they were, at all times, accompanied by parallel changes in [Ca2+](i).
UR - http://www.scopus.com/inward/record.url?scp=0034031995&partnerID=8YFLogxK
U2 - 10.1111/j.1469-445X.2000.00353.x
DO - 10.1111/j.1469-445X.2000.00353.x
M3 - Article
C2 - 10825423
AN - SCOPUS:0034031995
SN - 0958-0670
VL - 85
SP - 349
EP - 351
JO - Experimental Physiology
JF - Experimental Physiology
IS - 3
ER -