Silk fibroin/sericin native blends as potential biomaterial templates

Shuko Suzuki, Cassie L Rayner, Traian V. Chirila

Research output: Contribution to journalArticleResearchpeer-review

4 Downloads (Pure)

Abstract

The silk produced by the domesticated silkworm (Bombyx mori) consists of two major polypeptidic components, fibroin and sericin. Both are used as biomaterial templates in tissue engineering applications, with variable success. While fibroin membranes are mechanically strong, they do not promote satisfactorily attachment and proliferation of cells. Sericin membranes display a precisely opposite behavior. In order to generate silk–based templates with optimal cell–adhesive properties and acceptable mechanical strength, we propose a method to manufacture fibroin/sericin blends in one single step, by processing the whole silk cocoons. The membranes prepared from these “native” blends were physically characterized in this study, and assessed as substrata for the growth of retinal photoreceptor cells. The antioxidant effect putatively inducible by sericin was also evaluated. The blend membranes were found to be mechanically stronger than the fibroin membranes and led to significant enhancement of cell proliferation. Our method also prevented the advanced hydrothermal degradation of fibroin and sericin during processing. The membranes displayed both excellent transparency and suitable water content. However, their permeability was low, and no antioxidative activity related to the presence of sericin was detected when oxidative stress was induced in the cell cultures. The method developed in this study provides silk–based membranes with improved characteristics for tissue engineering applications.
Original languageEnglish
Pages (from-to)11-19
Number of pages9
JournalAdvances in Tissue Engineering and Regenerative Medicine
Volume5
Issue number1
DOIs
Publication statusPublished - Feb 2019
Externally publishedYes

Fingerprint

fibroins
biocompatible materials
silk
tissue engineering
cell proliferation
cocoons
Bombyx mori
silkworms
photoreceptors
sericin
permeability
manufacturing
cell culture
oxidative stress
antioxidant activity
methodology
water content
antioxidants
degradation

Cite this

Suzuki, Shuko ; Rayner, Cassie L ; Chirila, Traian V. / Silk fibroin/sericin native blends as potential biomaterial templates. In: Advances in Tissue Engineering and Regenerative Medicine. 2019 ; Vol. 5, No. 1. pp. 11-19.
@article{6f48fd20859d4c97ab20af5be8330455,
title = "Silk fibroin/sericin native blends as potential biomaterial templates",
abstract = "The silk produced by the domesticated silkworm (Bombyx mori) consists of two major polypeptidic components, fibroin and sericin. Both are used as biomaterial templates in tissue engineering applications, with variable success. While fibroin membranes are mechanically strong, they do not promote satisfactorily attachment and proliferation of cells. Sericin membranes display a precisely opposite behavior. In order to generate silk–based templates with optimal cell–adhesive properties and acceptable mechanical strength, we propose a method to manufacture fibroin/sericin blends in one single step, by processing the whole silk cocoons. The membranes prepared from these “native” blends were physically characterized in this study, and assessed as substrata for the growth of retinal photoreceptor cells. The antioxidant effect putatively inducible by sericin was also evaluated. The blend membranes were found to be mechanically stronger than the fibroin membranes and led to significant enhancement of cell proliferation. Our method also prevented the advanced hydrothermal degradation of fibroin and sericin during processing. The membranes displayed both excellent transparency and suitable water content. However, their permeability was low, and no antioxidative activity related to the presence of sericin was detected when oxidative stress was induced in the cell cultures. The method developed in this study provides silk–based membranes with improved characteristics for tissue engineering applications.",
author = "Shuko Suzuki and Rayner, {Cassie L} and Chirila, {Traian V.}",
year = "2019",
month = "2",
doi = "10.15406/atroa.2019.05.00093",
language = "English",
volume = "5",
pages = "11--19",
journal = "Advances in Tissue Engineering and Regenerative Medicine",
issn = "2572-8490",
publisher = "MedCrave Group, LLC",
number = "1",

}

Silk fibroin/sericin native blends as potential biomaterial templates. / Suzuki, Shuko; Rayner, Cassie L; Chirila, Traian V.

In: Advances in Tissue Engineering and Regenerative Medicine, Vol. 5, No. 1, 02.2019, p. 11-19.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Silk fibroin/sericin native blends as potential biomaterial templates

AU - Suzuki, Shuko

AU - Rayner, Cassie L

AU - Chirila, Traian V.

PY - 2019/2

Y1 - 2019/2

N2 - The silk produced by the domesticated silkworm (Bombyx mori) consists of two major polypeptidic components, fibroin and sericin. Both are used as biomaterial templates in tissue engineering applications, with variable success. While fibroin membranes are mechanically strong, they do not promote satisfactorily attachment and proliferation of cells. Sericin membranes display a precisely opposite behavior. In order to generate silk–based templates with optimal cell–adhesive properties and acceptable mechanical strength, we propose a method to manufacture fibroin/sericin blends in one single step, by processing the whole silk cocoons. The membranes prepared from these “native” blends were physically characterized in this study, and assessed as substrata for the growth of retinal photoreceptor cells. The antioxidant effect putatively inducible by sericin was also evaluated. The blend membranes were found to be mechanically stronger than the fibroin membranes and led to significant enhancement of cell proliferation. Our method also prevented the advanced hydrothermal degradation of fibroin and sericin during processing. The membranes displayed both excellent transparency and suitable water content. However, their permeability was low, and no antioxidative activity related to the presence of sericin was detected when oxidative stress was induced in the cell cultures. The method developed in this study provides silk–based membranes with improved characteristics for tissue engineering applications.

AB - The silk produced by the domesticated silkworm (Bombyx mori) consists of two major polypeptidic components, fibroin and sericin. Both are used as biomaterial templates in tissue engineering applications, with variable success. While fibroin membranes are mechanically strong, they do not promote satisfactorily attachment and proliferation of cells. Sericin membranes display a precisely opposite behavior. In order to generate silk–based templates with optimal cell–adhesive properties and acceptable mechanical strength, we propose a method to manufacture fibroin/sericin blends in one single step, by processing the whole silk cocoons. The membranes prepared from these “native” blends were physically characterized in this study, and assessed as substrata for the growth of retinal photoreceptor cells. The antioxidant effect putatively inducible by sericin was also evaluated. The blend membranes were found to be mechanically stronger than the fibroin membranes and led to significant enhancement of cell proliferation. Our method also prevented the advanced hydrothermal degradation of fibroin and sericin during processing. The membranes displayed both excellent transparency and suitable water content. However, their permeability was low, and no antioxidative activity related to the presence of sericin was detected when oxidative stress was induced in the cell cultures. The method developed in this study provides silk–based membranes with improved characteristics for tissue engineering applications.

U2 - 10.15406/atroa.2019.05.00093

DO - 10.15406/atroa.2019.05.00093

M3 - Article

VL - 5

SP - 11

EP - 19

JO - Advances in Tissue Engineering and Regenerative Medicine

JF - Advances in Tissue Engineering and Regenerative Medicine

SN - 2572-8490

IS - 1

ER -