Serotonin Levels in the Rabbit Retina Are Elevated Following Intraocular Injection of Forskolin

N. N. Osborne, N. L. Barnett

Research output: Contribution to journalArticleResearchpeer-review

17 Citations (Scopus)

Abstract

Abstract: Analysis of the mammalian retina for serotonin irnmu‐noreactivity suggests an absence of the amine. However, following an intraocular injection of forskolin (1 μM) into a rabbit eye 1 h before analysis of the retina, serotonin immunoreactivity is associated with a subpopulation of amacrine cells. These cells correspond in size and position to the “indoleamine‐accumulating cells” of the retina. Biochemical experiments show that forskolin treatment produces an increase in levels of endogenous serotonin and 5‐hydroxytrypto‐phan but has no effect on the uptake of serotonin or tryptophan or the metabolism of 5‐hydroxytryptophan. These results suggest that the “indoleamine‐accumulating cells” in the retina are “serotonergic cells” and that the level of amine is elevated sufficiently for localisation following forskolin treatment. It would appear that forskolin either directly or indirectly activates tryptophan hydroxylase.

Original languageEnglish
Pages (from-to)1955-1958
Number of pages4
JournalJournal of Neurochemistry
Volume53
Issue number6
DOIs
Publication statusPublished - 1 Jan 1989
Externally publishedYes

Fingerprint

Intraocular Injections
Colforsin
Retina
Serotonin
Rabbits
Amines
Tryptophan Hydroxylase
Amacrine Cells
Metabolism
Tryptophan
Therapeutics
Experiments

Cite this

@article{485940e0a930443bbef568f491f55d55,
title = "Serotonin Levels in the Rabbit Retina Are Elevated Following Intraocular Injection of Forskolin",
abstract = "Abstract: Analysis of the mammalian retina for serotonin irnmu‐noreactivity suggests an absence of the amine. However, following an intraocular injection of forskolin (1 μM) into a rabbit eye 1 h before analysis of the retina, serotonin immunoreactivity is associated with a subpopulation of amacrine cells. These cells correspond in size and position to the “indoleamine‐accumulating cells” of the retina. Biochemical experiments show that forskolin treatment produces an increase in levels of endogenous serotonin and 5‐hydroxytrypto‐phan but has no effect on the uptake of serotonin or tryptophan or the metabolism of 5‐hydroxytryptophan. These results suggest that the “indoleamine‐accumulating cells” in the retina are “serotonergic cells” and that the level of amine is elevated sufficiently for localisation following forskolin treatment. It would appear that forskolin either directly or indirectly activates tryptophan hydroxylase.",
author = "Osborne, {N. N.} and Barnett, {N. L.}",
year = "1989",
month = "1",
day = "1",
doi = "10.1111/j.1471-4159.1989.tb09267.x",
language = "English",
volume = "53",
pages = "1955--1958",
journal = "Journal of Neurochemistry",
issn = "0022-3042",
publisher = "Wiley-Blackwell",
number = "6",

}

Serotonin Levels in the Rabbit Retina Are Elevated Following Intraocular Injection of Forskolin. / Osborne, N. N.; Barnett, N. L.

In: Journal of Neurochemistry, Vol. 53, No. 6, 01.01.1989, p. 1955-1958.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Serotonin Levels in the Rabbit Retina Are Elevated Following Intraocular Injection of Forskolin

AU - Osborne, N. N.

AU - Barnett, N. L.

PY - 1989/1/1

Y1 - 1989/1/1

N2 - Abstract: Analysis of the mammalian retina for serotonin irnmu‐noreactivity suggests an absence of the amine. However, following an intraocular injection of forskolin (1 μM) into a rabbit eye 1 h before analysis of the retina, serotonin immunoreactivity is associated with a subpopulation of amacrine cells. These cells correspond in size and position to the “indoleamine‐accumulating cells” of the retina. Biochemical experiments show that forskolin treatment produces an increase in levels of endogenous serotonin and 5‐hydroxytrypto‐phan but has no effect on the uptake of serotonin or tryptophan or the metabolism of 5‐hydroxytryptophan. These results suggest that the “indoleamine‐accumulating cells” in the retina are “serotonergic cells” and that the level of amine is elevated sufficiently for localisation following forskolin treatment. It would appear that forskolin either directly or indirectly activates tryptophan hydroxylase.

AB - Abstract: Analysis of the mammalian retina for serotonin irnmu‐noreactivity suggests an absence of the amine. However, following an intraocular injection of forskolin (1 μM) into a rabbit eye 1 h before analysis of the retina, serotonin immunoreactivity is associated with a subpopulation of amacrine cells. These cells correspond in size and position to the “indoleamine‐accumulating cells” of the retina. Biochemical experiments show that forskolin treatment produces an increase in levels of endogenous serotonin and 5‐hydroxytrypto‐phan but has no effect on the uptake of serotonin or tryptophan or the metabolism of 5‐hydroxytryptophan. These results suggest that the “indoleamine‐accumulating cells” in the retina are “serotonergic cells” and that the level of amine is elevated sufficiently for localisation following forskolin treatment. It would appear that forskolin either directly or indirectly activates tryptophan hydroxylase.

UR - http://www.scopus.com/inward/record.url?scp=0024451826&partnerID=8YFLogxK

U2 - 10.1111/j.1471-4159.1989.tb09267.x

DO - 10.1111/j.1471-4159.1989.tb09267.x

M3 - Article

VL - 53

SP - 1955

EP - 1958

JO - Journal of Neurochemistry

JF - Journal of Neurochemistry

SN - 0022-3042

IS - 6

ER -