Serotonin Levels in the Rabbit Retina Are Elevated Following Intraocular Injection of Forskolin

N. N. Osborne*, N. L. Barnett

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

17 Citations (Scopus)


Abstract: Analysis of the mammalian retina for serotonin irnmu‐noreactivity suggests an absence of the amine. However, following an intraocular injection of forskolin (1 μM) into a rabbit eye 1 h before analysis of the retina, serotonin immunoreactivity is associated with a subpopulation of amacrine cells. These cells correspond in size and position to the “indoleamine‐accumulating cells” of the retina. Biochemical experiments show that forskolin treatment produces an increase in levels of endogenous serotonin and 5‐hydroxytrypto‐phan but has no effect on the uptake of serotonin or tryptophan or the metabolism of 5‐hydroxytryptophan. These results suggest that the “indoleamine‐accumulating cells” in the retina are “serotonergic cells” and that the level of amine is elevated sufficiently for localisation following forskolin treatment. It would appear that forskolin either directly or indirectly activates tryptophan hydroxylase.

Original languageEnglish
Pages (from-to)1955-1958
Number of pages4
JournalJournal of Neurochemistry
Issue number6
Publication statusPublished - 1 Jan 1989
Externally publishedYes


Cite this