Role of extracellular [Ca2+] in fatigue of isolated mammalian skeletal muscle

Simeon P. Cairns, Wayne A. Hing, John R. Slack, Roland G. Mills, Denis S. Loiselle

Research output: Contribution to journalArticleResearchpeer-review

32 Citations (Scopus)

Abstract

The possible role of altered extracellular Ca2+ concentration ([Ca2+](o)) in skeletal muscle fatigue was tested on isolated slow-twitch soleus and fast-twitch extensor digitorum longus muscles of the mouse. The following findings were made. 1) A change from the control solution (1.3 mM [Ca2+](o)) to 10 mM [Ca2+](o), or to nominally Ca2+-free solutions, had little effect on tetanic force in nonfatigued muscle. 2) Almost complete restoration of tetanic force was induced by 10 mM [Ca2+](o) in severely K+-depressed muscle (extracellular K+ concentration of 10-12 mM). This effect was attributed to a 5-mV reversal of the K+-induced depolarization and subsequent restoration of ability to generate action potentials (inferred by using the twitch force-stimulation strength relationship). 3) Tetanic force depressed by lowered extracellular Na+ concentration (40 mM) was further reduced with 10 mM [Ca2+](o). 4) Tetanic force loss at elevated extracellular K+ concentration (8 mM) and lowered extracellular Na+ concentration (100 mM) was partially reversed with 10 mM [Ca2+](o) or markedly exacerbated with low [Ca2+](o). 5) Fatigue induced by using repeated tetani in soleus was attenuated at 10 mM [Ca2+](o) (due to increased resting and evoked forces) and exacerbated at low [Ca2+](o). These combined results suggest, first, that raised [Ca2+](o) protects against fatigue rather than inducing it and, second, that a considerable depletion of [Ca2+](o) in the transverse tubules may contribute to fatigue.

Original languageEnglish
Pages (from-to)1395-1406
Number of pages12
JournalJournal of Applied Physiology
Volume84
Issue number4
DOIs
Publication statusPublished - 1 Apr 1998
Externally publishedYes

Fingerprint

Fatigue
Skeletal Muscle
Muscles
Muscle Fatigue
Tetanus
Action Potentials

Cite this

Cairns, Simeon P. ; Hing, Wayne A. ; Slack, John R. ; Mills, Roland G. ; Loiselle, Denis S. / Role of extracellular [Ca2+] in fatigue of isolated mammalian skeletal muscle. In: Journal of Applied Physiology. 1998 ; Vol. 84, No. 4. pp. 1395-1406.
@article{7cff17c60e8c42cc9d82cb6801b0397f,
title = "Role of extracellular [Ca2+] in fatigue of isolated mammalian skeletal muscle",
abstract = "The possible role of altered extracellular Ca2+ concentration ([Ca2+](o)) in skeletal muscle fatigue was tested on isolated slow-twitch soleus and fast-twitch extensor digitorum longus muscles of the mouse. The following findings were made. 1) A change from the control solution (1.3 mM [Ca2+](o)) to 10 mM [Ca2+](o), or to nominally Ca2+-free solutions, had little effect on tetanic force in nonfatigued muscle. 2) Almost complete restoration of tetanic force was induced by 10 mM [Ca2+](o) in severely K+-depressed muscle (extracellular K+ concentration of 10-12 mM). This effect was attributed to a 5-mV reversal of the K+-induced depolarization and subsequent restoration of ability to generate action potentials (inferred by using the twitch force-stimulation strength relationship). 3) Tetanic force depressed by lowered extracellular Na+ concentration (40 mM) was further reduced with 10 mM [Ca2+](o). 4) Tetanic force loss at elevated extracellular K+ concentration (8 mM) and lowered extracellular Na+ concentration (100 mM) was partially reversed with 10 mM [Ca2+](o) or markedly exacerbated with low [Ca2+](o). 5) Fatigue induced by using repeated tetani in soleus was attenuated at 10 mM [Ca2+](o) (due to increased resting and evoked forces) and exacerbated at low [Ca2+](o). These combined results suggest, first, that raised [Ca2+](o) protects against fatigue rather than inducing it and, second, that a considerable depletion of [Ca2+](o) in the transverse tubules may contribute to fatigue.",
author = "Cairns, {Simeon P.} and Hing, {Wayne A.} and Slack, {John R.} and Mills, {Roland G.} and Loiselle, {Denis S.}",
year = "1998",
month = "4",
day = "1",
doi = "10.1152/jappl.1998.84.4.1395",
language = "English",
volume = "84",
pages = "1395--1406",
journal = "Journal of Applied Physiology Respiratory Environmental and Exercise Physiology",
issn = "1522-1601",
publisher = "American Physiological Society",
number = "4",

}

Role of extracellular [Ca2+] in fatigue of isolated mammalian skeletal muscle. / Cairns, Simeon P.; Hing, Wayne A.; Slack, John R.; Mills, Roland G.; Loiselle, Denis S.

In: Journal of Applied Physiology, Vol. 84, No. 4, 01.04.1998, p. 1395-1406.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Role of extracellular [Ca2+] in fatigue of isolated mammalian skeletal muscle

AU - Cairns, Simeon P.

AU - Hing, Wayne A.

AU - Slack, John R.

AU - Mills, Roland G.

AU - Loiselle, Denis S.

PY - 1998/4/1

Y1 - 1998/4/1

N2 - The possible role of altered extracellular Ca2+ concentration ([Ca2+](o)) in skeletal muscle fatigue was tested on isolated slow-twitch soleus and fast-twitch extensor digitorum longus muscles of the mouse. The following findings were made. 1) A change from the control solution (1.3 mM [Ca2+](o)) to 10 mM [Ca2+](o), or to nominally Ca2+-free solutions, had little effect on tetanic force in nonfatigued muscle. 2) Almost complete restoration of tetanic force was induced by 10 mM [Ca2+](o) in severely K+-depressed muscle (extracellular K+ concentration of 10-12 mM). This effect was attributed to a 5-mV reversal of the K+-induced depolarization and subsequent restoration of ability to generate action potentials (inferred by using the twitch force-stimulation strength relationship). 3) Tetanic force depressed by lowered extracellular Na+ concentration (40 mM) was further reduced with 10 mM [Ca2+](o). 4) Tetanic force loss at elevated extracellular K+ concentration (8 mM) and lowered extracellular Na+ concentration (100 mM) was partially reversed with 10 mM [Ca2+](o) or markedly exacerbated with low [Ca2+](o). 5) Fatigue induced by using repeated tetani in soleus was attenuated at 10 mM [Ca2+](o) (due to increased resting and evoked forces) and exacerbated at low [Ca2+](o). These combined results suggest, first, that raised [Ca2+](o) protects against fatigue rather than inducing it and, second, that a considerable depletion of [Ca2+](o) in the transverse tubules may contribute to fatigue.

AB - The possible role of altered extracellular Ca2+ concentration ([Ca2+](o)) in skeletal muscle fatigue was tested on isolated slow-twitch soleus and fast-twitch extensor digitorum longus muscles of the mouse. The following findings were made. 1) A change from the control solution (1.3 mM [Ca2+](o)) to 10 mM [Ca2+](o), or to nominally Ca2+-free solutions, had little effect on tetanic force in nonfatigued muscle. 2) Almost complete restoration of tetanic force was induced by 10 mM [Ca2+](o) in severely K+-depressed muscle (extracellular K+ concentration of 10-12 mM). This effect was attributed to a 5-mV reversal of the K+-induced depolarization and subsequent restoration of ability to generate action potentials (inferred by using the twitch force-stimulation strength relationship). 3) Tetanic force depressed by lowered extracellular Na+ concentration (40 mM) was further reduced with 10 mM [Ca2+](o). 4) Tetanic force loss at elevated extracellular K+ concentration (8 mM) and lowered extracellular Na+ concentration (100 mM) was partially reversed with 10 mM [Ca2+](o) or markedly exacerbated with low [Ca2+](o). 5) Fatigue induced by using repeated tetani in soleus was attenuated at 10 mM [Ca2+](o) (due to increased resting and evoked forces) and exacerbated at low [Ca2+](o). These combined results suggest, first, that raised [Ca2+](o) protects against fatigue rather than inducing it and, second, that a considerable depletion of [Ca2+](o) in the transverse tubules may contribute to fatigue.

UR - http://www.scopus.com/inward/record.url?scp=0031594359&partnerID=8YFLogxK

U2 - 10.1152/jappl.1998.84.4.1395

DO - 10.1152/jappl.1998.84.4.1395

M3 - Article

VL - 84

SP - 1395

EP - 1406

JO - Journal of Applied Physiology Respiratory Environmental and Exercise Physiology

JF - Journal of Applied Physiology Respiratory Environmental and Exercise Physiology

SN - 1522-1601

IS - 4

ER -