Regulation of albumin endocytosis by PSD95/Dlg/ZO-1 (PDZ) scaffolds

Interaction of Na+-H+ exchange regulatory factor-2 with ClC-5

Deanne H. Hryciw, Jenny Ekberg, Charles Ferguson, Aven Lee, Dongsheng Wang, Robert G. Parton, Carol A. Pollock, Chris C. Yun, Philip Poronnik

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Abstract

The constitutive reuptake of albumin from the glomerular filtrate by receptor-mediated endocytosis is a key function of the renal proximal tubules. Both the Cl- channel ClC-5 and the Na+-H + exchanger isoform 3 are critical components of the macromolecular endocytic complex that is required for albumin uptake, and therefore the cell-surface levels of these proteins may limit albumin endocytosis. This study was undertaken to investigate the potential roles of the epithelial PDZ scaffolds, Na+-H+ exchange regulatory factors, NHERF1 and NHERF2, in albumin uptake by opossum kidney (OK) cells. We found that ClC-5 co-immunoprecipitates with NHERF2 but not NHERF1 from OK cell lysate. Experiments using fusion proteins demonstrated that this was a direct interaction between an internal binding site in the C terminus of ClC-5 and the PDZ2 module of NHERF2. In OK cells, NHERF2 is restricted to the intravillar region while NHERF1 is located in the microvilli. Silencing NHERF2 reduced both cell-surface levels of ClC-5 and albumin uptake. Conversely, silencing NHERF1 increased cell-surface levels of ClC-5 and albumin uptake, presumably by increasing the mobility of NHE3 in the membrane and its availability to the albumin uptake complex. Surface biotinylation experiments revealed that both NHERF1 and NHERF2 were associated with the plasma membrane and that NHERF2 was recruited to the membrane in the presence of albumin. The importance of the interaction between NHERF2 and the cytoskeleton was demonstrated by a significant reduction in albumin uptake in cells overexpressing an ezrin binding-deficient mutant of NHERF2. Thus NHERF1 and NHERF2 differentially regulate albumin uptake by mechanisms that ultimately alter the cell-surface levels of ClC-5.

Original languageEnglish
Pages (from-to)16068-16077
Number of pages10
JournalJournal of Biological Chemistry
Volume281
Issue number23
DOIs
Publication statusPublished - 9 Jun 2006
Externally publishedYes

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Endocytosis
Scaffolds
Albumins
Opossums
Kidney
Biotinylation
Membranes
Macromolecular Substances
Proximal Kidney Tubule
Sodium-Hydrogen Antiporter
Cell membranes
Microvilli
Cytoskeleton
Protein Isoforms
Membrane Proteins
Proteins
Fusion reactions
Experiments
Binding Sites
Cell Membrane

Cite this

Hryciw, Deanne H. ; Ekberg, Jenny ; Ferguson, Charles ; Lee, Aven ; Wang, Dongsheng ; Parton, Robert G. ; Pollock, Carol A. ; Yun, Chris C. ; Poronnik, Philip. / Regulation of albumin endocytosis by PSD95/Dlg/ZO-1 (PDZ) scaffolds : Interaction of Na+-H+ exchange regulatory factor-2 with ClC-5. In: Journal of Biological Chemistry. 2006 ; Vol. 281, No. 23. pp. 16068-16077.
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title = "Regulation of albumin endocytosis by PSD95/Dlg/ZO-1 (PDZ) scaffolds: Interaction of Na+-H+ exchange regulatory factor-2 with ClC-5",
abstract = "The constitutive reuptake of albumin from the glomerular filtrate by receptor-mediated endocytosis is a key function of the renal proximal tubules. Both the Cl- channel ClC-5 and the Na+-H + exchanger isoform 3 are critical components of the macromolecular endocytic complex that is required for albumin uptake, and therefore the cell-surface levels of these proteins may limit albumin endocytosis. This study was undertaken to investigate the potential roles of the epithelial PDZ scaffolds, Na+-H+ exchange regulatory factors, NHERF1 and NHERF2, in albumin uptake by opossum kidney (OK) cells. We found that ClC-5 co-immunoprecipitates with NHERF2 but not NHERF1 from OK cell lysate. Experiments using fusion proteins demonstrated that this was a direct interaction between an internal binding site in the C terminus of ClC-5 and the PDZ2 module of NHERF2. In OK cells, NHERF2 is restricted to the intravillar region while NHERF1 is located in the microvilli. Silencing NHERF2 reduced both cell-surface levels of ClC-5 and albumin uptake. Conversely, silencing NHERF1 increased cell-surface levels of ClC-5 and albumin uptake, presumably by increasing the mobility of NHE3 in the membrane and its availability to the albumin uptake complex. Surface biotinylation experiments revealed that both NHERF1 and NHERF2 were associated with the plasma membrane and that NHERF2 was recruited to the membrane in the presence of albumin. The importance of the interaction between NHERF2 and the cytoskeleton was demonstrated by a significant reduction in albumin uptake in cells overexpressing an ezrin binding-deficient mutant of NHERF2. Thus NHERF1 and NHERF2 differentially regulate albumin uptake by mechanisms that ultimately alter the cell-surface levels of ClC-5.",
author = "Hryciw, {Deanne H.} and Jenny Ekberg and Charles Ferguson and Aven Lee and Dongsheng Wang and Parton, {Robert G.} and Pollock, {Carol A.} and Yun, {Chris C.} and Philip Poronnik",
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Hryciw, DH, Ekberg, J, Ferguson, C, Lee, A, Wang, D, Parton, RG, Pollock, CA, Yun, CC & Poronnik, P 2006, 'Regulation of albumin endocytosis by PSD95/Dlg/ZO-1 (PDZ) scaffolds: Interaction of Na+-H+ exchange regulatory factor-2 with ClC-5', Journal of Biological Chemistry, vol. 281, no. 23, pp. 16068-16077. https://doi.org/10.1074/jbc.M512559200

Regulation of albumin endocytosis by PSD95/Dlg/ZO-1 (PDZ) scaffolds : Interaction of Na+-H+ exchange regulatory factor-2 with ClC-5. / Hryciw, Deanne H.; Ekberg, Jenny; Ferguson, Charles; Lee, Aven; Wang, Dongsheng; Parton, Robert G.; Pollock, Carol A.; Yun, Chris C.; Poronnik, Philip.

In: Journal of Biological Chemistry, Vol. 281, No. 23, 09.06.2006, p. 16068-16077.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - Regulation of albumin endocytosis by PSD95/Dlg/ZO-1 (PDZ) scaffolds

T2 - Interaction of Na+-H+ exchange regulatory factor-2 with ClC-5

AU - Hryciw, Deanne H.

AU - Ekberg, Jenny

AU - Ferguson, Charles

AU - Lee, Aven

AU - Wang, Dongsheng

AU - Parton, Robert G.

AU - Pollock, Carol A.

AU - Yun, Chris C.

AU - Poronnik, Philip

PY - 2006/6/9

Y1 - 2006/6/9

N2 - The constitutive reuptake of albumin from the glomerular filtrate by receptor-mediated endocytosis is a key function of the renal proximal tubules. Both the Cl- channel ClC-5 and the Na+-H + exchanger isoform 3 are critical components of the macromolecular endocytic complex that is required for albumin uptake, and therefore the cell-surface levels of these proteins may limit albumin endocytosis. This study was undertaken to investigate the potential roles of the epithelial PDZ scaffolds, Na+-H+ exchange regulatory factors, NHERF1 and NHERF2, in albumin uptake by opossum kidney (OK) cells. We found that ClC-5 co-immunoprecipitates with NHERF2 but not NHERF1 from OK cell lysate. Experiments using fusion proteins demonstrated that this was a direct interaction between an internal binding site in the C terminus of ClC-5 and the PDZ2 module of NHERF2. In OK cells, NHERF2 is restricted to the intravillar region while NHERF1 is located in the microvilli. Silencing NHERF2 reduced both cell-surface levels of ClC-5 and albumin uptake. Conversely, silencing NHERF1 increased cell-surface levels of ClC-5 and albumin uptake, presumably by increasing the mobility of NHE3 in the membrane and its availability to the albumin uptake complex. Surface biotinylation experiments revealed that both NHERF1 and NHERF2 were associated with the plasma membrane and that NHERF2 was recruited to the membrane in the presence of albumin. The importance of the interaction between NHERF2 and the cytoskeleton was demonstrated by a significant reduction in albumin uptake in cells overexpressing an ezrin binding-deficient mutant of NHERF2. Thus NHERF1 and NHERF2 differentially regulate albumin uptake by mechanisms that ultimately alter the cell-surface levels of ClC-5.

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