Reduced resting skeletal muscle protein synthesis is rescued by resistance exercise and protein ingestion following short-term energy deficit

Jose L. Areta, Louise M. Burke, Donny M. Camera, Daniel W D West, Siobhan Crawshay, Daniel R. Moore, Trent Stellingwerff, Stuart M. Phillips, John A. Hawley*, Vernon G. Coffey

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

69 Citations (Scopus)

Abstract

The myofibrillar protein synthesis (MPS) response to resistance exercise (REX) and protein ingestion during energy deficit (ED) is unknown. In young men (n = 8) and women (n = 7), we determined protein signaling and resting postabsorptive MPS during energy balance [EB; 45 kcal.kg fat-free mass (FFM)(-1).day(-1)] and after 5 days of ED (30 kcal.kg FFM-1.day(-1)) as well as MPS while in ED after acute REX in the fasted state and with the ingestion of whey protein (15 and 30 g). Postabsorptive rates of MPS were 27% lower in ED than EB (P <0.001), but REX stimulated MPS to rates equal to EB. Ingestion of 15 and 30 g of protein after REX in ED increased MPS similar to 16 and similar to 34% above resting EB (P <0.02). p70 S6K Thr(389) phosphorylation increased above EB only with combined exercise and protein intake (similar to 2-7 fold, P <0.05). In conclusion, short-term ED reduces postabsorptive MPS; however, a bout of REX in ED restores MPS to values observed at rest in EB. The ingestion of protein after REX further increases MPS above resting EB in a dose-dependent manner. We conclude that combining REX with increased protein availability after exercise enhances rates of skeletal muscle protein synthesis during short-term ED and could in the long term preserve muscle mass.

Original languageEnglish
Pages (from-to)E989-E997
Number of pages9
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume306
Issue number8
DOIs
Publication statusPublished - Apr 2014
Externally publishedYes

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