Reduced resting skeletal muscle protein synthesis is rescued by resistance exercise and protein ingestion following short-term energy deficit

Jose L. Areta, Louise M. Burke, Donny M. Camera, Daniel W D West, Siobhan Crawshay, Daniel R. Moore, Trent Stellingwerff, Stuart M. Phillips, John A. Hawley, Vernon G. Coffey

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Abstract

The myofibrillar protein synthesis (MPS) response to resistance exercise (REX) and protein ingestion during energy deficit (ED) is unknown. In young men (n = 8) and women (n = 7), we determined protein signaling and resting postabsorptive MPS during energy balance [EB; 45 kcal.kg fat-free mass (FFM)(-1).day(-1)] and after 5 days of ED (30 kcal.kg FFM-1.day(-1)) as well as MPS while in ED after acute REX in the fasted state and with the ingestion of whey protein (15 and 30 g). Postabsorptive rates of MPS were 27% lower in ED than EB (P <0.001), but REX stimulated MPS to rates equal to EB. Ingestion of 15 and 30 g of protein after REX in ED increased MPS similar to 16 and similar to 34% above resting EB (P <0.02). p70 S6K Thr(389) phosphorylation increased above EB only with combined exercise and protein intake (similar to 2-7 fold, P <0.05). In conclusion, short-term ED reduces postabsorptive MPS; however, a bout of REX in ED restores MPS to values observed at rest in EB. The ingestion of protein after REX further increases MPS above resting EB in a dose-dependent manner. We conclude that combining REX with increased protein availability after exercise enhances rates of skeletal muscle protein synthesis during short-term ED and could in the long term preserve muscle mass.

Original languageEnglish
Pages (from-to)E989-E997
Number of pages9
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume306
Issue number8
DOIs
Publication statusPublished - Apr 2014
Externally publishedYes

Cite this

Areta, Jose L. ; Burke, Louise M. ; Camera, Donny M. ; West, Daniel W D ; Crawshay, Siobhan ; Moore, Daniel R. ; Stellingwerff, Trent ; Phillips, Stuart M. ; Hawley, John A. ; Coffey, Vernon G. / Reduced resting skeletal muscle protein synthesis is rescued by resistance exercise and protein ingestion following short-term energy deficit. In: American Journal of Physiology - Endocrinology and Metabolism. 2014 ; Vol. 306, No. 8. pp. E989-E997.
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abstract = "The myofibrillar protein synthesis (MPS) response to resistance exercise (REX) and protein ingestion during energy deficit (ED) is unknown. In young men (n = 8) and women (n = 7), we determined protein signaling and resting postabsorptive MPS during energy balance [EB; 45 kcal.kg fat-free mass (FFM)(-1).day(-1)] and after 5 days of ED (30 kcal.kg FFM-1.day(-1)) as well as MPS while in ED after acute REX in the fasted state and with the ingestion of whey protein (15 and 30 g). Postabsorptive rates of MPS were 27{\%} lower in ED than EB (P <0.001), but REX stimulated MPS to rates equal to EB. Ingestion of 15 and 30 g of protein after REX in ED increased MPS similar to 16 and similar to 34{\%} above resting EB (P <0.02). p70 S6K Thr(389) phosphorylation increased above EB only with combined exercise and protein intake (similar to 2-7 fold, P <0.05). In conclusion, short-term ED reduces postabsorptive MPS; however, a bout of REX in ED restores MPS to values observed at rest in EB. The ingestion of protein after REX further increases MPS above resting EB in a dose-dependent manner. We conclude that combining REX with increased protein availability after exercise enhances rates of skeletal muscle protein synthesis during short-term ED and could in the long term preserve muscle mass.",
author = "Areta, {Jose L.} and Burke, {Louise M.} and Camera, {Donny M.} and West, {Daniel W D} and Siobhan Crawshay and Moore, {Daniel R.} and Trent Stellingwerff and Phillips, {Stuart M.} and Hawley, {John A.} and Coffey, {Vernon G.}",
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Reduced resting skeletal muscle protein synthesis is rescued by resistance exercise and protein ingestion following short-term energy deficit. / Areta, Jose L.; Burke, Louise M.; Camera, Donny M.; West, Daniel W D; Crawshay, Siobhan; Moore, Daniel R.; Stellingwerff, Trent; Phillips, Stuart M.; Hawley, John A.; Coffey, Vernon G.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 306, No. 8, 04.2014, p. E989-E997.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Reduced resting skeletal muscle protein synthesis is rescued by resistance exercise and protein ingestion following short-term energy deficit

AU - Areta, Jose L.

AU - Burke, Louise M.

AU - Camera, Donny M.

AU - West, Daniel W D

AU - Crawshay, Siobhan

AU - Moore, Daniel R.

AU - Stellingwerff, Trent

AU - Phillips, Stuart M.

AU - Hawley, John A.

AU - Coffey, Vernon G.

PY - 2014/4

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N2 - The myofibrillar protein synthesis (MPS) response to resistance exercise (REX) and protein ingestion during energy deficit (ED) is unknown. In young men (n = 8) and women (n = 7), we determined protein signaling and resting postabsorptive MPS during energy balance [EB; 45 kcal.kg fat-free mass (FFM)(-1).day(-1)] and after 5 days of ED (30 kcal.kg FFM-1.day(-1)) as well as MPS while in ED after acute REX in the fasted state and with the ingestion of whey protein (15 and 30 g). Postabsorptive rates of MPS were 27% lower in ED than EB (P <0.001), but REX stimulated MPS to rates equal to EB. Ingestion of 15 and 30 g of protein after REX in ED increased MPS similar to 16 and similar to 34% above resting EB (P <0.02). p70 S6K Thr(389) phosphorylation increased above EB only with combined exercise and protein intake (similar to 2-7 fold, P <0.05). In conclusion, short-term ED reduces postabsorptive MPS; however, a bout of REX in ED restores MPS to values observed at rest in EB. The ingestion of protein after REX further increases MPS above resting EB in a dose-dependent manner. We conclude that combining REX with increased protein availability after exercise enhances rates of skeletal muscle protein synthesis during short-term ED and could in the long term preserve muscle mass.

AB - The myofibrillar protein synthesis (MPS) response to resistance exercise (REX) and protein ingestion during energy deficit (ED) is unknown. In young men (n = 8) and women (n = 7), we determined protein signaling and resting postabsorptive MPS during energy balance [EB; 45 kcal.kg fat-free mass (FFM)(-1).day(-1)] and after 5 days of ED (30 kcal.kg FFM-1.day(-1)) as well as MPS while in ED after acute REX in the fasted state and with the ingestion of whey protein (15 and 30 g). Postabsorptive rates of MPS were 27% lower in ED than EB (P <0.001), but REX stimulated MPS to rates equal to EB. Ingestion of 15 and 30 g of protein after REX in ED increased MPS similar to 16 and similar to 34% above resting EB (P <0.02). p70 S6K Thr(389) phosphorylation increased above EB only with combined exercise and protein intake (similar to 2-7 fold, P <0.05). In conclusion, short-term ED reduces postabsorptive MPS; however, a bout of REX in ED restores MPS to values observed at rest in EB. The ingestion of protein after REX further increases MPS above resting EB in a dose-dependent manner. We conclude that combining REX with increased protein availability after exercise enhances rates of skeletal muscle protein synthesis during short-term ED and could in the long term preserve muscle mass.

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DO - 10.1152/ajpendo.00590.2013

M3 - Article

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SP - E989-E997

JO - American Journal of Physiology - Endocrinology and Metabolism

JF - American Journal of Physiology - Endocrinology and Metabolism

SN - 0193-1849

IS - 8

ER -