Spleen is known to contain multiple dendritic and myeloid cell subsets, distinguishable on the basis of phenotype, function and anatomical location. As a result of recent intensive flow cytometric analyses, splenic dendritic cell (DC) subsets are now better characterised than other myeloid subsets. In order to identify and fully characterise a novel splenic subset termed 'L-DC' in relation to other myeloid cells, it was necessary to investigate myeloid subsets in more detail. In terms of cell surface phenotype, L-DC were initially characterised as a CD11bhiCD11cloMHCII−Ly6C−Ly6G− subset in murine spleen. Their expression of CD43, lack of MHCII, and a low level of CD11c was shown to best differentiate L-DC by phenotype from conventional DC subsets. A complete analysis of all subsets in spleen led to the classification of CD11bhiCD11cloMHCII−Ly6CloLy6G− cells as monocytes expressing CX3CR1, CD43 and CD115. Siglec-F expression was used to identify a specific eosinophil population, distinguishable from both Ly6Clo and Ly6Chi monocytes, and other DC subsets. L-DC were characterised as a clear subset of CD11bhiCD11cloMHCII−Ly6C−Ly6G− cells, which are CD43+, Siglec-F− and CD115−. Changes in the prevalence of L-DC compared to other subsets in spleens of mutant mice confirmed the phenotypic distinction between L-DC, cDC and monocyte subsets. L-DC development in vivo was shown to occur independently of the BATF3 transcription factor that regulates cDC development, and also independently of the FLT3L and GM-CSF growth factors which drive cDC and monocyte development, so distinguishing L-DC from these commonly defined cell types.
|Publication status||Published - Nov 2016|
|Event||The 55th Australian Society for Medical Research National Scientific Conference: Next Generation Healthcare: Merging Biology & Technology - Bond University, Gold Coast, Australia|
Duration: 13 Nov 2016 → 15 Nov 2016
Conference number: 55th
|Conference||The 55th Australian Society for Medical Research National Scientific Conference|
|Abbreviated title||ASMR NSC|
|Period||13/11/16 → 15/11/16|
Hey, Y., & O'Neill, H. C. (2016). Redefining splenic dendritic and myeloid cell subsets. Poster session presented at The 55th Australian Society for Medical Research National Scientific Conference, Gold Coast, Australia.