Redefining myeloid cell subsets in murine spleen

Research output: Contribution to journalMeeting AbstractResearch

Abstract

Spleen is known to contain multiple dendritic and myeloid cell subsets, distinguishable on the basis ofphenotype, function and anatomical location. As a result of recent intensive flow cytometric analyses,splenic dendritic cell (DC) subsets are now better characterised than other myeloid subsets. In order toidentify and fully characterise a novel splenic subset termed 'L-DC' in relation to other myeloid cells, itwas necessary to investigate myeloid subsets in more detail. In terms of cell surface phenotype, L-DCwere initially characterised as a CD11bhiCD11cloMHCII−Ly6C−Ly6G− subset in murine spleen. Theirexpression of CD43, lack of MHCII, and a low level of CD11c was shown to best differentiate L-DC byphenotype from conventional DC subsets. A complete analysis of all subsets in spleen led to theclassification of CD11bhiCD11cloMHCII−Ly6CloLy6G− cells as monocytes expressing CX3CR1, CD43and CD115. Siglec-F expression was used to identify a specific eosinophil population, distinguishablefrom both Ly6Clo and Ly6Chi monocytes, and other DC subsets. L-DC were characterised as a clearsubset of CD11bhiCD11cloMHCII−Ly6C−Ly6G− cells, which are CD43+, Siglec-F− and CD115−.Changes in the prevalence of L-DC compared to other subsets in spleens of mutant mice confirmedthe phenotypic distinction between L-DC, cDC and monocyte subsets. L-DC development in vivo wasshown to occur independently of the BATF3 transcription factor that regulates cDC development, andalso independently of the FLT3L and GM-CSF growth factors which drive cDC and monocytedevelopment, so distinguishing L-DC from these commonly defined cell types.
Original languageEnglish
Article number1007
Pages (from-to)249-249
Number of pages1
JournalEuropean Journal of Immunology
Volume46
Issue numberS1
DOIs
Publication statusPublished - Aug 2016
EventInternational Congress of Immunology (ICI) - Melbourne, Australia
Duration: 21 Aug 201626 Aug 2016

Cite this

@article{313474e91e3b44b7a6fd6b39f14666db,
title = "Redefining myeloid cell subsets in murine spleen",
abstract = "Spleen is known to contain multiple dendritic and myeloid cell subsets, distinguishable on the basis ofphenotype, function and anatomical location. As a result of recent intensive flow cytometric analyses,splenic dendritic cell (DC) subsets are now better characterised than other myeloid subsets. In order toidentify and fully characterise a novel splenic subset termed 'L-DC' in relation to other myeloid cells, itwas necessary to investigate myeloid subsets in more detail. In terms of cell surface phenotype, L-DCwere initially characterised as a CD11bhiCD11cloMHCII−Ly6C−Ly6G− subset in murine spleen. Theirexpression of CD43, lack of MHCII, and a low level of CD11c was shown to best differentiate L-DC byphenotype from conventional DC subsets. A complete analysis of all subsets in spleen led to theclassification of CD11bhiCD11cloMHCII−Ly6CloLy6G− cells as monocytes expressing CX3CR1, CD43and CD115. Siglec-F expression was used to identify a specific eosinophil population, distinguishablefrom both Ly6Clo and Ly6Chi monocytes, and other DC subsets. L-DC were characterised as a clearsubset of CD11bhiCD11cloMHCII−Ly6C−Ly6G− cells, which are CD43+, Siglec-F− and CD115−.Changes in the prevalence of L-DC compared to other subsets in spleens of mutant mice confirmedthe phenotypic distinction between L-DC, cDC and monocyte subsets. L-DC development in vivo wasshown to occur independently of the BATF3 transcription factor that regulates cDC development, andalso independently of the FLT3L and GM-CSF growth factors which drive cDC and monocytedevelopment, so distinguishing L-DC from these commonly defined cell types.",
author = "Hey, {Y. Y.} and Tan, {J. K. H.} and O'Neill, {H. C.}",
year = "2016",
month = "8",
doi = "10.1002/eji.201670200",
language = "English",
volume = "46",
pages = "249--249",
journal = "European Journal of Immunology",
issn = "0014-2980",
publisher = "Wiley-Blackwell",
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}

Redefining myeloid cell subsets in murine spleen. / Hey, Y. Y.; Tan, J. K. H.; O'Neill, H. C.

In: European Journal of Immunology, Vol. 46, No. S1, 1007, 08.2016, p. 249-249.

Research output: Contribution to journalMeeting AbstractResearch

TY - JOUR

T1 - Redefining myeloid cell subsets in murine spleen

AU - Hey, Y. Y.

AU - Tan, J. K. H.

AU - O'Neill, H. C.

PY - 2016/8

Y1 - 2016/8

N2 - Spleen is known to contain multiple dendritic and myeloid cell subsets, distinguishable on the basis ofphenotype, function and anatomical location. As a result of recent intensive flow cytometric analyses,splenic dendritic cell (DC) subsets are now better characterised than other myeloid subsets. In order toidentify and fully characterise a novel splenic subset termed 'L-DC' in relation to other myeloid cells, itwas necessary to investigate myeloid subsets in more detail. In terms of cell surface phenotype, L-DCwere initially characterised as a CD11bhiCD11cloMHCII−Ly6C−Ly6G− subset in murine spleen. Theirexpression of CD43, lack of MHCII, and a low level of CD11c was shown to best differentiate L-DC byphenotype from conventional DC subsets. A complete analysis of all subsets in spleen led to theclassification of CD11bhiCD11cloMHCII−Ly6CloLy6G− cells as monocytes expressing CX3CR1, CD43and CD115. Siglec-F expression was used to identify a specific eosinophil population, distinguishablefrom both Ly6Clo and Ly6Chi monocytes, and other DC subsets. L-DC were characterised as a clearsubset of CD11bhiCD11cloMHCII−Ly6C−Ly6G− cells, which are CD43+, Siglec-F− and CD115−.Changes in the prevalence of L-DC compared to other subsets in spleens of mutant mice confirmedthe phenotypic distinction between L-DC, cDC and monocyte subsets. L-DC development in vivo wasshown to occur independently of the BATF3 transcription factor that regulates cDC development, andalso independently of the FLT3L and GM-CSF growth factors which drive cDC and monocytedevelopment, so distinguishing L-DC from these commonly defined cell types.

AB - Spleen is known to contain multiple dendritic and myeloid cell subsets, distinguishable on the basis ofphenotype, function and anatomical location. As a result of recent intensive flow cytometric analyses,splenic dendritic cell (DC) subsets are now better characterised than other myeloid subsets. In order toidentify and fully characterise a novel splenic subset termed 'L-DC' in relation to other myeloid cells, itwas necessary to investigate myeloid subsets in more detail. In terms of cell surface phenotype, L-DCwere initially characterised as a CD11bhiCD11cloMHCII−Ly6C−Ly6G− subset in murine spleen. Theirexpression of CD43, lack of MHCII, and a low level of CD11c was shown to best differentiate L-DC byphenotype from conventional DC subsets. A complete analysis of all subsets in spleen led to theclassification of CD11bhiCD11cloMHCII−Ly6CloLy6G− cells as monocytes expressing CX3CR1, CD43and CD115. Siglec-F expression was used to identify a specific eosinophil population, distinguishablefrom both Ly6Clo and Ly6Chi monocytes, and other DC subsets. L-DC were characterised as a clearsubset of CD11bhiCD11cloMHCII−Ly6C−Ly6G− cells, which are CD43+, Siglec-F− and CD115−.Changes in the prevalence of L-DC compared to other subsets in spleens of mutant mice confirmedthe phenotypic distinction between L-DC, cDC and monocyte subsets. L-DC development in vivo wasshown to occur independently of the BATF3 transcription factor that regulates cDC development, andalso independently of the FLT3L and GM-CSF growth factors which drive cDC and monocytedevelopment, so distinguishing L-DC from these commonly defined cell types.

U2 - 10.1002/eji.201670200

DO - 10.1002/eji.201670200

M3 - Meeting Abstract

VL - 46

SP - 249

EP - 249

JO - European Journal of Immunology

JF - European Journal of Immunology

SN - 0014-2980

IS - S1

M1 - 1007

ER -