Rapid loss of glutamine synthetase from astrocytes in response to hypoxia: Implications for excitotoxicity

Aven Lee, Barbara E. Lingwood, S. Tracey Bjorkman, Stephanie M. Miller, Philip Poronnik, Nigel L. Barnett, Paul Colditz, David V. Pow*

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

31 Citations (Scopus)

Abstract

We have examined brains of neonatal pigs that were rendered hypoxic. Glutamine synthetase (GS), a key enzyme in the detoxification of glutamate and ammonia, was rapidly lost from astrocytes in regions susceptible to damage, including the CA1 of hippocampus and various cortical regions. Conversely, resilient areas such as the dentate gyrus exhibited little or no loss of GS. Onset of loss was rapid, patches of loss being evident by 1 h post-insult, and loss was extensive by 24 h and did not recover by 72 h. Examination of counterstained sections revealed that GS losses preceded any overt neuronal damage. Loss of GS from astrocytes would plausibly lead to a rise in intracellular glutamate, and could explain why reversal of astrocytic glutamate transport during hypoxia/ischaemia is conceptually possible.

Original languageEnglish
Pages (from-to)211-220
Number of pages10
JournalJournal of Chemical Neuroanatomy
Volume39
Issue number3
DOIs
Publication statusPublished - 1 May 2010
Externally publishedYes

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