Quantitative variation in H-2-antigen expression: Estimation of H-2K and H-2D expression in different strains of mice

Helen C O'Neill, I F McKenzie

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22 Citations (Scopus)

Abstract

Minor differences in the expression of individual H-2K and H-2D antigens were detected on mouse spleen cells. The method involved the use of an 125I-protein A radioimmunoassay using highly specific anti-H-2 sera to make estimates of the number of cell-bound antibody molecules. The maximum number of antibody binding sites varied for each H-2 antigen reflecting differences of between 10 and 70 percent in the expression of any two antigens. The order of magnitude of expression was Db greater than (Kd)=Kk=Kb=Dq greater than Dd greater than Kq greater than Dk. Minor background differences were detectable, but antigen expression was allele-specific and independent of the expression of other K, D or I antigens expressed on the same cell.

Original languageEnglish
Pages (from-to)225-239
Number of pages15
JournalImmunogenetics
Volume11
Issue number1
DOIs
Publication statusPublished - 1980
Externally publishedYes

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H-2 Antigens
Antibody Binding Sites
Antigens
Staphylococcal Protein A
Radioimmunoassay
Spleen
Cell Count
Alleles
Antibodies
Serum
H antigen
I-antigen
K antigens

Cite this

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title = "Quantitative variation in H-2-antigen expression: Estimation of H-2K and H-2D expression in different strains of mice",
abstract = "Minor differences in the expression of individual H-2K and H-2D antigens were detected on mouse spleen cells. The method involved the use of an 125I-protein A radioimmunoassay using highly specific anti-H-2 sera to make estimates of the number of cell-bound antibody molecules. The maximum number of antibody binding sites varied for each H-2 antigen reflecting differences of between 10 and 70 percent in the expression of any two antigens. The order of magnitude of expression was Db greater than (Kd)=Kk=Kb=Dq greater than Dd greater than Kq greater than Dk. Minor background differences were detectable, but antigen expression was allele-specific and independent of the expression of other K, D or I antigens expressed on the same cell.",
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Quantitative variation in H-2-antigen expression : Estimation of H-2K and H-2D expression in different strains of mice. / O'Neill, Helen C; McKenzie, I F.

In: Immunogenetics, Vol. 11, No. 1, 1980, p. 225-239.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Quantitative variation in H-2-antigen expression

T2 - Estimation of H-2K and H-2D expression in different strains of mice

AU - O'Neill, Helen C

AU - McKenzie, I F

PY - 1980

Y1 - 1980

N2 - Minor differences in the expression of individual H-2K and H-2D antigens were detected on mouse spleen cells. The method involved the use of an 125I-protein A radioimmunoassay using highly specific anti-H-2 sera to make estimates of the number of cell-bound antibody molecules. The maximum number of antibody binding sites varied for each H-2 antigen reflecting differences of between 10 and 70 percent in the expression of any two antigens. The order of magnitude of expression was Db greater than (Kd)=Kk=Kb=Dq greater than Dd greater than Kq greater than Dk. Minor background differences were detectable, but antigen expression was allele-specific and independent of the expression of other K, D or I antigens expressed on the same cell.

AB - Minor differences in the expression of individual H-2K and H-2D antigens were detected on mouse spleen cells. The method involved the use of an 125I-protein A radioimmunoassay using highly specific anti-H-2 sera to make estimates of the number of cell-bound antibody molecules. The maximum number of antibody binding sites varied for each H-2 antigen reflecting differences of between 10 and 70 percent in the expression of any two antigens. The order of magnitude of expression was Db greater than (Kd)=Kk=Kb=Dq greater than Dd greater than Kq greater than Dk. Minor background differences were detectable, but antigen expression was allele-specific and independent of the expression of other K, D or I antigens expressed on the same cell.

U2 - 10.1007/BF01567790

DO - 10.1007/BF01567790

M3 - Article

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JO - Immunogenetics

JF - Immunogenetics

SN - 0093-7711

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