TY - CHAP
T1 - Quantitative (Real-Time) RT-PCR in cardiovascular research
AU - Ashton, Kevin John
AU - Headrick, John Patrick
PY - 2007/3/19
Y1 - 2007/3/19
N2 - Quantitative (real-time) PCR (qPCR) represents a highly sensitive, sequence-specific, and reproducible technique for the gel-free detection and quantitation of nucleic acids. Owing to its large dynamic range and throughput, this approach has become the chosen method for rapid quantification of mRNA levels in biological samples. The sensitivity of this method permits the reliable detection of low concentrations of initial template, while delivering a linear range of up to 10 orders of magnitude in copy number. This chapter details the basic methodology behind key components of a qPCR experiment, including sample preparation, fluorescent chemistries, primer/probe design, and data analysis applicable to cardiovascular research.
AB - Quantitative (real-time) PCR (qPCR) represents a highly sensitive, sequence-specific, and reproducible technique for the gel-free detection and quantitation of nucleic acids. Owing to its large dynamic range and throughput, this approach has become the chosen method for rapid quantification of mRNA levels in biological samples. The sensitivity of this method permits the reliable detection of low concentrations of initial template, while delivering a linear range of up to 10 orders of magnitude in copy number. This chapter details the basic methodology behind key components of a qPCR experiment, including sample preparation, fluorescent chemistries, primer/probe design, and data analysis applicable to cardiovascular research.
UR - http://www.scopus.com/inward/record.url?scp=34447272461&partnerID=8YFLogxK
U2 - 10.1385/1-59745-030-8:121
DO - 10.1385/1-59745-030-8:121
M3 - Chapter
C2 - 17568122
AN - SCOPUS:34447272461
SN - 1597450308
SN - 9781597450300
VL - 366
T3 - Methods in Molecular Biology
SP - 121
EP - 143
BT - Cardiac Gene Expression: Methods and Protocols
ER -