Protein kinase C-mediated modulation of glutamate transporter activity in rat retina

Natalie D. Bull, John P. Wood, Neville N. Osborne, Nigel L. Barnett

Research output: Contribution to journalArticleResearchpeer-review

2 Citations (Scopus)

Abstract

It has previously been shown that inhibitors of protein kinase C (PKC) attenuate retinal glutamate uptake in situ. The aim of the current study was to determine whether PKCδ -mediated inhibition differentially reduces the transport of glutamate into retinal Müller cells when compared with retinal neurons. The influence of two different types of PKC inhibitors on the uptake of [3H]D-aspartate was therefore compared in the intact retina, mixed retinal cultures, and Müller cell-enriched retinal cultures. It was found that 25 μM of the pan-isoform PKC inhibitor, chelerythrine, reduced [3H]D-aspartate uptake by 78%, 71%, and 68% in isolated retinas, mixed neuronal/glial cultures, and Müller cell-enriched cultures, respectively. Importantly, 20 μM of the PKCδ -selective inhibitor rottlerin also reduced the uptake of D-aspartate to similar extents in all three systems, and the reductions were statistically similar to those found for the pan-specific PKC inhibitor. Neither pan-isoform nor PKCδ -selective activators stimulated glutamate uptake in either culture system or the intact retina. The current results suggest that specific PKC inhibitors are quantitatively similar in reducing the uptake of glutamate into retinal neurons and Müller cells.

Original languageEnglish
Pages (from-to)123-131
Number of pages9
JournalCurrent Eye Research
Volume32
Issue number2
DOIs
Publication statusPublished - 1 Feb 2007
Externally publishedYes

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Amino Acid Transport System X-AG
Protein Kinase C
Retina
Protein C Inhibitor
Protein Kinase Inhibitors
D-Aspartic Acid
Glutamic Acid
Retinal Neurons
Protein Isoforms
Cell Culture Techniques
Neuroglia

Cite this

Bull, Natalie D. ; Wood, John P. ; Osborne, Neville N. ; Barnett, Nigel L. / Protein kinase C-mediated modulation of glutamate transporter activity in rat retina. In: Current Eye Research. 2007 ; Vol. 32, No. 2. pp. 123-131.
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abstract = "It has previously been shown that inhibitors of protein kinase C (PKC) attenuate retinal glutamate uptake in situ. The aim of the current study was to determine whether PKCδ -mediated inhibition differentially reduces the transport of glutamate into retinal M{\"u}ller cells when compared with retinal neurons. The influence of two different types of PKC inhibitors on the uptake of [3H]D-aspartate was therefore compared in the intact retina, mixed retinal cultures, and M{\"u}ller cell-enriched retinal cultures. It was found that 25 μM of the pan-isoform PKC inhibitor, chelerythrine, reduced [3H]D-aspartate uptake by 78{\%}, 71{\%}, and 68{\%} in isolated retinas, mixed neuronal/glial cultures, and M{\"u}ller cell-enriched cultures, respectively. Importantly, 20 μM of the PKCδ -selective inhibitor rottlerin also reduced the uptake of D-aspartate to similar extents in all three systems, and the reductions were statistically similar to those found for the pan-specific PKC inhibitor. Neither pan-isoform nor PKCδ -selective activators stimulated glutamate uptake in either culture system or the intact retina. The current results suggest that specific PKC inhibitors are quantitatively similar in reducing the uptake of glutamate into retinal neurons and M{\"u}ller cells.",
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Protein kinase C-mediated modulation of glutamate transporter activity in rat retina. / Bull, Natalie D.; Wood, John P.; Osborne, Neville N.; Barnett, Nigel L.

In: Current Eye Research, Vol. 32, No. 2, 01.02.2007, p. 123-131.

Research output: Contribution to journalArticleResearchpeer-review

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