Preferential usage of the V beta 8 gene family by CD4-CD8-T cell lines derived from spleen

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Abstract

Receptors encoded by the V beta 8 gene family and identified by the F23.1 antibody are commonly expressed amongst the CD4-CD8-T cell lines isolated from spleen cells infected in vitro with the RadLV retrovirus. All but one out of 12 cell lines showed between 50 and 85% F23.1+ cells in the uncloned cell population which is noticably higher than the approximately 13% level amongst the Ig- normal spleen cell population. There was a high frequency (approximately 50%) of F23.1+ clones from five of these cell lines. The frequency of F23.1 binding cells in the Ig-, CD4/CD8-depleted spleen population is only 0.2%, which gives a precursor frequency in spleen of less than 0.002%. This reflects selective isolation of CD4-CD8- alpha beta+ cells which express V beta 8 gene products by this culture scheme. The requirement for RadLV in induction of these cell lines has been established, suggesting that this retrovirus may selectively stimulate CD4-CD8-F23.1+ T cells. These cells may represent an autoimmune subset present in peripheral lymphoid tissue.

Original languageEnglish
Pages (from-to)256-264
Number of pages9
JournalCellular Immunology
Volume129
Issue number1
DOIs
Publication statusPublished - Aug 1990
Externally publishedYes

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Spleen
T-Lymphocytes
Cell Line
Radiation Leukemia Virus
Genes
Retroviridae
Population
Lymphoid Tissue
Clone Cells
Antibodies

Cite this

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title = "Preferential usage of the V beta 8 gene family by CD4-CD8-T cell lines derived from spleen",
abstract = "Receptors encoded by the V beta 8 gene family and identified by the F23.1 antibody are commonly expressed amongst the CD4-CD8-T cell lines isolated from spleen cells infected in vitro with the RadLV retrovirus. All but one out of 12 cell lines showed between 50 and 85{\%} F23.1+ cells in the uncloned cell population which is noticably higher than the approximately 13{\%} level amongst the Ig- normal spleen cell population. There was a high frequency (approximately 50{\%}) of F23.1+ clones from five of these cell lines. The frequency of F23.1 binding cells in the Ig-, CD4/CD8-depleted spleen population is only 0.2{\%}, which gives a precursor frequency in spleen of less than 0.002{\%}. This reflects selective isolation of CD4-CD8- alpha beta+ cells which express V beta 8 gene products by this culture scheme. The requirement for RadLV in induction of these cell lines has been established, suggesting that this retrovirus may selectively stimulate CD4-CD8-F23.1+ T cells. These cells may represent an autoimmune subset present in peripheral lymphoid tissue.",
author = "O'Neill, {H C}",
year = "1990",
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doi = "10.1016/0008-8749(90)90202-3",
language = "English",
volume = "129",
pages = "256--264",
journal = "Cellular Immunology",
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}

Preferential usage of the V beta 8 gene family by CD4-CD8-T cell lines derived from spleen. / O'Neill, H C.

In: Cellular Immunology, Vol. 129, No. 1, 08.1990, p. 256-264.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Preferential usage of the V beta 8 gene family by CD4-CD8-T cell lines derived from spleen

AU - O'Neill, H C

PY - 1990/8

Y1 - 1990/8

N2 - Receptors encoded by the V beta 8 gene family and identified by the F23.1 antibody are commonly expressed amongst the CD4-CD8-T cell lines isolated from spleen cells infected in vitro with the RadLV retrovirus. All but one out of 12 cell lines showed between 50 and 85% F23.1+ cells in the uncloned cell population which is noticably higher than the approximately 13% level amongst the Ig- normal spleen cell population. There was a high frequency (approximately 50%) of F23.1+ clones from five of these cell lines. The frequency of F23.1 binding cells in the Ig-, CD4/CD8-depleted spleen population is only 0.2%, which gives a precursor frequency in spleen of less than 0.002%. This reflects selective isolation of CD4-CD8- alpha beta+ cells which express V beta 8 gene products by this culture scheme. The requirement for RadLV in induction of these cell lines has been established, suggesting that this retrovirus may selectively stimulate CD4-CD8-F23.1+ T cells. These cells may represent an autoimmune subset present in peripheral lymphoid tissue.

AB - Receptors encoded by the V beta 8 gene family and identified by the F23.1 antibody are commonly expressed amongst the CD4-CD8-T cell lines isolated from spleen cells infected in vitro with the RadLV retrovirus. All but one out of 12 cell lines showed between 50 and 85% F23.1+ cells in the uncloned cell population which is noticably higher than the approximately 13% level amongst the Ig- normal spleen cell population. There was a high frequency (approximately 50%) of F23.1+ clones from five of these cell lines. The frequency of F23.1 binding cells in the Ig-, CD4/CD8-depleted spleen population is only 0.2%, which gives a precursor frequency in spleen of less than 0.002%. This reflects selective isolation of CD4-CD8- alpha beta+ cells which express V beta 8 gene products by this culture scheme. The requirement for RadLV in induction of these cell lines has been established, suggesting that this retrovirus may selectively stimulate CD4-CD8-F23.1+ T cells. These cells may represent an autoimmune subset present in peripheral lymphoid tissue.

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JF - Cellular Immunology

SN - 0008-8749

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