Preexercise aminoacidemia and muscle protein synthesis after resistance exercise

Louise M. Burke, John A. Hawley, Megan L. Ross, Daniel R. Moore, Stuart M. Phillips, Gary R. Slater, Trent Stellingwerff, Kevin D. Tipton, Andrew P. Garnham, Vernon G. Coffey

Research output: Contribution to journalArticleResearchpeer-review

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Abstract

PURPOSE: We have previously shown that the aminoacidemia caused by the consumption of a rapidly digested protein after resistance exercise enhances muscle protein synthesis (MPS) more than the amino acid (AA) profile associated with a slowly digested protein. Here, we investigated whether differential feeding patterns of a whey protein mixture commencing before exercise affect postexercise intracellular signaling and MPS. METHODS: Twelve resistance-trained males performed leg resistance exercise 45 min after commencing each of three volume-matched nutrition protocols: placebo (PLAC, artificially sweetened water), BOLUS (25 g of whey protein + 5 g of leucine dissolved in artificially sweetened water; 1× 500 mL), or PULSE (15× 33-mL aliquots of BOLUS drink every 15 min). RESULTS: The preexercise rise in plasma AA concentration with PULSE was attenuated compared with BOLUS (P < 0.05); this effect was reversed after exercise, with two-fold greater leucine concentrations in PULSE compared with BOLUS (P < 0.05). One-hour postexercise, phosphorylation of p70 S6K and rpS6 was increased above baseline with BOLUS and PULSE, but not PLAC (P < 0.05); furthermore, PULSE > BOLUS (P < 0.05). MPS throughout 5 h of recovery was higher with protein ingestion compared with PLAC (0.037 ± 0.007), with no differences between BOLUS or PULSE (0.085 ± 0.013 vs. 0.095 ± 0.010%•h, respectively, P = 0.56). CONCLUSIONS: Manipulation of aminoacidemia before resistance exercise via different patterns of intake of protein altered plasma AA profiles and postexercise intracellular signaling. However, there was no difference in the enhancement of the muscle protein synthetic response after exercise. Protein sources producing a slow AA release, when consumed before resistance exercise in sufficient amounts, are as effective as rapidly digested proteins in promoting postexercise MPS.

Original languageEnglish
Pages (from-to)1968-1977
Number of pages10
JournalMedicine and Science in Sports and Exercise
Volume44
Issue number10
DOIs
Publication statusPublished - Oct 2012
Externally publishedYes

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Muscle Proteins
Amino Acids
Proteins
Intracellular Signaling Peptides and Proteins
Water
Feeding Behavior
Leucine
Blood Proteins
Leg
Eating
Placebos
Whey Proteins
5'-palmitoyl cytarabine

Cite this

Burke, Louise M. ; Hawley, John A. ; Ross, Megan L. ; Moore, Daniel R. ; Phillips, Stuart M. ; Slater, Gary R. ; Stellingwerff, Trent ; Tipton, Kevin D. ; Garnham, Andrew P. ; Coffey, Vernon G. / Preexercise aminoacidemia and muscle protein synthesis after resistance exercise. In: Medicine and Science in Sports and Exercise. 2012 ; Vol. 44, No. 10. pp. 1968-1977.
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title = "Preexercise aminoacidemia and muscle protein synthesis after resistance exercise",
abstract = "PURPOSE: We have previously shown that the aminoacidemia caused by the consumption of a rapidly digested protein after resistance exercise enhances muscle protein synthesis (MPS) more than the amino acid (AA) profile associated with a slowly digested protein. Here, we investigated whether differential feeding patterns of a whey protein mixture commencing before exercise affect postexercise intracellular signaling and MPS. METHODS: Twelve resistance-trained males performed leg resistance exercise 45 min after commencing each of three volume-matched nutrition protocols: placebo (PLAC, artificially sweetened water), BOLUS (25 g of whey protein + 5 g of leucine dissolved in artificially sweetened water; 1× 500 mL), or PULSE (15× 33-mL aliquots of BOLUS drink every 15 min). RESULTS: The preexercise rise in plasma AA concentration with PULSE was attenuated compared with BOLUS (P < 0.05); this effect was reversed after exercise, with two-fold greater leucine concentrations in PULSE compared with BOLUS (P < 0.05). One-hour postexercise, phosphorylation of p70 S6K and rpS6 was increased above baseline with BOLUS and PULSE, but not PLAC (P < 0.05); furthermore, PULSE > BOLUS (P < 0.05). MPS throughout 5 h of recovery was higher with protein ingestion compared with PLAC (0.037 ± 0.007), with no differences between BOLUS or PULSE (0.085 ± 0.013 vs. 0.095 ± 0.010{\%}•h, respectively, P = 0.56). CONCLUSIONS: Manipulation of aminoacidemia before resistance exercise via different patterns of intake of protein altered plasma AA profiles and postexercise intracellular signaling. However, there was no difference in the enhancement of the muscle protein synthetic response after exercise. Protein sources producing a slow AA release, when consumed before resistance exercise in sufficient amounts, are as effective as rapidly digested proteins in promoting postexercise MPS.",
author = "Burke, {Louise M.} and Hawley, {John A.} and Ross, {Megan L.} and Moore, {Daniel R.} and Phillips, {Stuart M.} and Slater, {Gary R.} and Trent Stellingwerff and Tipton, {Kevin D.} and Garnham, {Andrew P.} and Coffey, {Vernon G.}",
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Burke, LM, Hawley, JA, Ross, ML, Moore, DR, Phillips, SM, Slater, GR, Stellingwerff, T, Tipton, KD, Garnham, AP & Coffey, VG 2012, 'Preexercise aminoacidemia and muscle protein synthesis after resistance exercise' Medicine and Science in Sports and Exercise, vol. 44, no. 10, pp. 1968-1977. https://doi.org/10.1249/MSS.0b013e31825d28fa

Preexercise aminoacidemia and muscle protein synthesis after resistance exercise. / Burke, Louise M.; Hawley, John A.; Ross, Megan L.; Moore, Daniel R.; Phillips, Stuart M.; Slater, Gary R.; Stellingwerff, Trent; Tipton, Kevin D.; Garnham, Andrew P.; Coffey, Vernon G.

In: Medicine and Science in Sports and Exercise, Vol. 44, No. 10, 10.2012, p. 1968-1977.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Preexercise aminoacidemia and muscle protein synthesis after resistance exercise

AU - Burke, Louise M.

AU - Hawley, John A.

AU - Ross, Megan L.

AU - Moore, Daniel R.

AU - Phillips, Stuart M.

AU - Slater, Gary R.

AU - Stellingwerff, Trent

AU - Tipton, Kevin D.

AU - Garnham, Andrew P.

AU - Coffey, Vernon G.

PY - 2012/10

Y1 - 2012/10

N2 - PURPOSE: We have previously shown that the aminoacidemia caused by the consumption of a rapidly digested protein after resistance exercise enhances muscle protein synthesis (MPS) more than the amino acid (AA) profile associated with a slowly digested protein. Here, we investigated whether differential feeding patterns of a whey protein mixture commencing before exercise affect postexercise intracellular signaling and MPS. METHODS: Twelve resistance-trained males performed leg resistance exercise 45 min after commencing each of three volume-matched nutrition protocols: placebo (PLAC, artificially sweetened water), BOLUS (25 g of whey protein + 5 g of leucine dissolved in artificially sweetened water; 1× 500 mL), or PULSE (15× 33-mL aliquots of BOLUS drink every 15 min). RESULTS: The preexercise rise in plasma AA concentration with PULSE was attenuated compared with BOLUS (P < 0.05); this effect was reversed after exercise, with two-fold greater leucine concentrations in PULSE compared with BOLUS (P < 0.05). One-hour postexercise, phosphorylation of p70 S6K and rpS6 was increased above baseline with BOLUS and PULSE, but not PLAC (P < 0.05); furthermore, PULSE > BOLUS (P < 0.05). MPS throughout 5 h of recovery was higher with protein ingestion compared with PLAC (0.037 ± 0.007), with no differences between BOLUS or PULSE (0.085 ± 0.013 vs. 0.095 ± 0.010%•h, respectively, P = 0.56). CONCLUSIONS: Manipulation of aminoacidemia before resistance exercise via different patterns of intake of protein altered plasma AA profiles and postexercise intracellular signaling. However, there was no difference in the enhancement of the muscle protein synthetic response after exercise. Protein sources producing a slow AA release, when consumed before resistance exercise in sufficient amounts, are as effective as rapidly digested proteins in promoting postexercise MPS.

AB - PURPOSE: We have previously shown that the aminoacidemia caused by the consumption of a rapidly digested protein after resistance exercise enhances muscle protein synthesis (MPS) more than the amino acid (AA) profile associated with a slowly digested protein. Here, we investigated whether differential feeding patterns of a whey protein mixture commencing before exercise affect postexercise intracellular signaling and MPS. METHODS: Twelve resistance-trained males performed leg resistance exercise 45 min after commencing each of three volume-matched nutrition protocols: placebo (PLAC, artificially sweetened water), BOLUS (25 g of whey protein + 5 g of leucine dissolved in artificially sweetened water; 1× 500 mL), or PULSE (15× 33-mL aliquots of BOLUS drink every 15 min). RESULTS: The preexercise rise in plasma AA concentration with PULSE was attenuated compared with BOLUS (P < 0.05); this effect was reversed after exercise, with two-fold greater leucine concentrations in PULSE compared with BOLUS (P < 0.05). One-hour postexercise, phosphorylation of p70 S6K and rpS6 was increased above baseline with BOLUS and PULSE, but not PLAC (P < 0.05); furthermore, PULSE > BOLUS (P < 0.05). MPS throughout 5 h of recovery was higher with protein ingestion compared with PLAC (0.037 ± 0.007), with no differences between BOLUS or PULSE (0.085 ± 0.013 vs. 0.095 ± 0.010%•h, respectively, P = 0.56). CONCLUSIONS: Manipulation of aminoacidemia before resistance exercise via different patterns of intake of protein altered plasma AA profiles and postexercise intracellular signaling. However, there was no difference in the enhancement of the muscle protein synthetic response after exercise. Protein sources producing a slow AA release, when consumed before resistance exercise in sufficient amounts, are as effective as rapidly digested proteins in promoting postexercise MPS.

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EP - 1977

JO - Medicine and science in sports

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