Platinum Induced Fatty Acids: Are they Present in Plasma of Cancer Patients?

Vera C Mazurak, Barbara S Van der Meij, Rachel Murphy, A. Stanislaus, V. V. Damaraju, Q. Chu, Michael Sawyer

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Abstract

Rationale: Preclinical studies suggest that hexadeca-4,7,10,13-tetraenoic [16:4 (n-3)] induces resistance to platinum-based therapies. One study in healthy volunteers reported increases in 16:4(n-3) after consuming fish oil with levels returning to normal after 12 hours. No study has measured in 16:4(n-3) levels in non-small cell lung cancer (NSCLC) patients receiving platinum based chemotherapy.
This study aimed to determine plasma levels of 16:4(n-3) in NSCLC pts undergoing platinum-based chemotherapy in those using or not using fish oil supplements.
Methods: A method to measure 16:4(n-3) in plasma of NSCLC patients was developed and validated. Patients using fish oil (capsules or oil equivalent, n=15) and on standard of care (n=5) were selected for preliminary analysis. Plasma was prospectively collected at baseline (prior to receiving chemotherapy) and after 2 cycles of platinum-based chemotherapy. The plasma range of 16:4(n-3) at baseline and subsequently following chemotherapy were compared between those taking fish oil and those on standard of care. Plasma (80 μl) fatty acids were extracted with iso-octane, derivatized and analyzed by LC-MS/MS in positive ion mode, which enabled detection of fatty acids at <1nmol/L.
Results: At baseline, before starting chemotherapy or fish oil supplementation, all patients had detectable levels of 16:4(n-3) ranging from 8 to 160 nmol/L. Fish oil users had higher absolute values of 16:4(n-3) than standard of care (mean=47 vs 23 nmol/L; p=0.03). Changes in 16:4(n-3) concentrations after platinum based therapy were highly variable with some patients increasing and others decreasing plasma 16:4(n-3).
Conclusion: These preliminary results suggest that 16:4(n-3) may be present in NSCLC patients both before and after receiving platinum-based chemotherapy. It will be important to relate concentrations of 16:4(n-3) to chemotherapy response and survival in a larger sample of cancer patients.
Original languageEnglish
Pages (from-to)S47
Number of pages1
JournalClinical Nutrition
Volume36
Issue numberS1
DOIs
Publication statusPublished - Sep 2017
EventThe 39th European Society for Clinical Nutrition and Metabolism Congress 2017 - Hague, Netherlands
Duration: 9 Sep 201712 Sep 2017
Conference number: 39
http://www.espen.org/congress/next-congresses/12-espen/index.php?option=com_content&view=article&id=214&Itemid=1052

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Mazurak, V. C., Van der Meij, B. S., Murphy, R., Stanislaus, A., V. Damaraju, V., Chu, Q., & Sawyer, M. (2017). Platinum Induced Fatty Acids: Are they Present in Plasma of Cancer Patients? Clinical Nutrition, 36(S1), S47. https://doi.org/10.1016/S0261-5614(17)30673-8