Phasic contractions in urinary bladder from juvenile versus adult pigs

Bahareh Vahabi, Donna J. Sellers, Dominika A. Bijos, Marcus J. Drake

Research output: Contribution to journalArticleResearchpeer-review

11 Citations (Scopus)

Abstract

Aims: Alterations in properties of the bladder with maturation are relevant physiologically and pathophysiologically. The aim of this study was to investigate alterations in bladder properties with maturation in juvenile vs. adult pig, focussing on differences between layers of the bladder wall (mucosa vs. detrusor) and the presence and functional contribution of interstitial cells (ICs). Methods: Basal and cholinergic-induced phasic contractions (PCs) in mucosal and denuded-detrusor strips from juvenile and adult pigs were assessed. Expression of c-kit, a marker of ICs, was investigated in the mucosa and the detrusor layers of the pig bladder. The functional role of ICs in mediating PCs was examined using imatinib. Results: Mucosal strips from juvenile and adult pig bladders demonstrated basal PCs whilst denuded-detrusor strips did not. PCs of mucosal strips from juvenile pigs were significantly greater than those from adult bladders. Immunoreactivity for c-kit was detected in mucosa and detrusor layers of pig bladder. Histological studies demonstrated a distinct layer of smooth muscle between the urothelium and bladder detrusor, termed the muscularis mucosa. Imatinib was only effective in inhibiting PCs in mucosal strips from juvenile pigs. Imatinib inhibited the carbachol-induced PCs of both juvenile and adult denuded-detrusor strips, although strips from juvenile bladders demonstrated a trend towards being more sensitive to this inhibition. Conclusions: We confirm the presence of c-kit positive ICs in pig urinary bladder. The enhanced PCs of mucosal strips from juvenile animals could be due to altered properties of ICs or the muscularis mucosa in the bladders of these animals.

Original languageEnglish
Article numbere58611
Number of pages10
JournalPLoS One
Volume8
Issue number3
DOIs
Publication statusPublished - 13 Mar 2013

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bladder
Urinary Bladder
Swine
swine
mucosa
Mucous Membrane
Animals
cells
Carbachol
Cholinergic Agents
Muscle
carbachol
Urothelium
cholinergic agents
smooth muscle
Smooth Muscle
animals
Imatinib Mesylate

Cite this

Vahabi, Bahareh ; Sellers, Donna J. ; Bijos, Dominika A. ; Drake, Marcus J. / Phasic contractions in urinary bladder from juvenile versus adult pigs. In: PLoS One. 2013 ; Vol. 8, No. 3.
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Phasic contractions in urinary bladder from juvenile versus adult pigs. / Vahabi, Bahareh; Sellers, Donna J.; Bijos, Dominika A.; Drake, Marcus J.

In: PLoS One, Vol. 8, No. 3, e58611, 13.03.2013.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Sellers, Donna J.

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N2 - Aims: Alterations in properties of the bladder with maturation are relevant physiologically and pathophysiologically. The aim of this study was to investigate alterations in bladder properties with maturation in juvenile vs. adult pig, focussing on differences between layers of the bladder wall (mucosa vs. detrusor) and the presence and functional contribution of interstitial cells (ICs). Methods: Basal and cholinergic-induced phasic contractions (PCs) in mucosal and denuded-detrusor strips from juvenile and adult pigs were assessed. Expression of c-kit, a marker of ICs, was investigated in the mucosa and the detrusor layers of the pig bladder. The functional role of ICs in mediating PCs was examined using imatinib. Results: Mucosal strips from juvenile and adult pig bladders demonstrated basal PCs whilst denuded-detrusor strips did not. PCs of mucosal strips from juvenile pigs were significantly greater than those from adult bladders. Immunoreactivity for c-kit was detected in mucosa and detrusor layers of pig bladder. Histological studies demonstrated a distinct layer of smooth muscle between the urothelium and bladder detrusor, termed the muscularis mucosa. Imatinib was only effective in inhibiting PCs in mucosal strips from juvenile pigs. Imatinib inhibited the carbachol-induced PCs of both juvenile and adult denuded-detrusor strips, although strips from juvenile bladders demonstrated a trend towards being more sensitive to this inhibition. Conclusions: We confirm the presence of c-kit positive ICs in pig urinary bladder. The enhanced PCs of mucosal strips from juvenile animals could be due to altered properties of ICs or the muscularis mucosa in the bladders of these animals.

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