Pharmacologic responses of the mouse urinary bladder

A. Erdem Canda, Christopher R. Chapple, Russ Chess-Williams

Research output: Contribution to journalArticleResearchpeer-review

4 Citations (Scopus)

Abstract

The aim of the study was to determine pathways involved in contraction and relaxation of the mouse urinary bladder. Mouse bladder strips were set up in gassed Krebs-bicarbonate solution and responses to various drugs and electrical field stimulation were obtained. Isoprenaline (b-receptor agonist) caused a 63% inhibition of carbachol precontracted detrusor (EC50=2nM). Carbachol caused contraction (EC50=0.3μM), responses were antagonised more potently by 4-DAMP (M3-antagonist) than methoctramine (M2-antagonist). Electrical field stimulation caused contraction, which was inhibited by atropine (60%) and less by guanethidine and α,β-methylene-ATP. The neurogenic responses were not potentiated by inhibition of nitric oxide synthase. Presence of an intact urothelium significantly depressed responses to carbachol (p=0.02) and addition of indomethacin and L-NNA to remove prostaglandin and nitric oxide production respectively did not prevent the inhibitory effect of the urothelium. In conclusion, b-receptor agonists cause relaxation and muscarinic agonists cause contraction via the M3-receptor. Acetylcholine is the main neurotransmitter causing contraction while nitric oxide has a minor role. The mouse and human urothelium are similar in releasing a factor that inhibits contraction of the detrusor muscle which is unidentified but is not nitric oxide or a prostaglandin. Therefore, the mouse may be used as a model to study the lower urinary tract.

Original languageEnglish
Pages (from-to)192-197
Number of pages6
JournalCentral European Journal of Medicine
Volume4
Issue number2
DOIs
Publication statusPublished - Jun 2009
Externally publishedYes

Fingerprint

Urothelium
Urinary Bladder
Carbachol
Nitric Oxide
Electric Stimulation
Prostaglandins
Guanethidine
Muscarinic Agonists
Bicarbonates
Muscle Contraction
Urinary Tract
Atropine
Isoproterenol
Nitric Oxide Synthase
Indomethacin
Acetylcholine
Neurotransmitter Agents
Adenosine Triphosphate
Pharmaceutical Preparations

Cite this

Canda, A. Erdem ; Chapple, Christopher R. ; Chess-Williams, Russ. / Pharmacologic responses of the mouse urinary bladder. In: Central European Journal of Medicine. 2009 ; Vol. 4, No. 2. pp. 192-197.
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Pharmacologic responses of the mouse urinary bladder. / Canda, A. Erdem; Chapple, Christopher R.; Chess-Williams, Russ.

In: Central European Journal of Medicine, Vol. 4, No. 2, 06.2009, p. 192-197.

Research output: Contribution to journalArticleResearchpeer-review

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