Novel splenic antigen-presenting cells derive from a Lin(-) c-kit(lo) progenitor

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Abstract

The main DC subsets in murine spleen arise from BM-derived precursors. Recently, a novel APC type was described in spleen. To determine if spleen contains the progenitors of this subset, a stromal coculture system was used to assess candidate progenitors for their hematopoietic potential. Here, the progenitor of that subset is identified as a spleen endogenous Lin(-)c-kit(lo) hematopoietic progenitor and is most highly enriched among the Lin(-)c-kit(lo)CD34(+) subset. Dendritic-like cells produced in vitro functionally resemble the previously described in vivo equivalent subset with high endocytic capacity and capability for antigen-specific activation of CD8(+) T cells but not CD4(+) T cells.

Original languageEnglish
Pages (from-to)63-9
Number of pages7
JournalJournal of Leukocyte Biology
Volume93
Issue number1
DOIs
Publication statusPublished - Jan 2013
Externally publishedYes

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Antigen-Presenting Cells
Spleen
T-Lymphocytes
Coculture Techniques
Dendritic Cells
Antigens

Cite this

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title = "Novel splenic antigen-presenting cells derive from a Lin(-) c-kit(lo) progenitor",
abstract = "The main DC subsets in murine spleen arise from BM-derived precursors. Recently, a novel APC type was described in spleen. To determine if spleen contains the progenitors of this subset, a stromal coculture system was used to assess candidate progenitors for their hematopoietic potential. Here, the progenitor of that subset is identified as a spleen endogenous Lin(-)c-kit(lo) hematopoietic progenitor and is most highly enriched among the Lin(-)c-kit(lo)CD34(+) subset. Dendritic-like cells produced in vitro functionally resemble the previously described in vivo equivalent subset with high endocytic capacity and capability for antigen-specific activation of CD8(+) T cells but not CD4(+) T cells.",
author = "Pravin Periasamy and Tan, {Jonathan K H} and O'Neill, {Helen C}",
year = "2013",
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Novel splenic antigen-presenting cells derive from a Lin(-) c-kit(lo) progenitor. / Periasamy, Pravin; Tan, Jonathan K H; O'Neill, Helen C.

In: Journal of Leukocyte Biology, Vol. 93, No. 1, 01.2013, p. 63-9.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Novel splenic antigen-presenting cells derive from a Lin(-) c-kit(lo) progenitor

AU - Periasamy, Pravin

AU - Tan, Jonathan K H

AU - O'Neill, Helen C

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N2 - The main DC subsets in murine spleen arise from BM-derived precursors. Recently, a novel APC type was described in spleen. To determine if spleen contains the progenitors of this subset, a stromal coculture system was used to assess candidate progenitors for their hematopoietic potential. Here, the progenitor of that subset is identified as a spleen endogenous Lin(-)c-kit(lo) hematopoietic progenitor and is most highly enriched among the Lin(-)c-kit(lo)CD34(+) subset. Dendritic-like cells produced in vitro functionally resemble the previously described in vivo equivalent subset with high endocytic capacity and capability for antigen-specific activation of CD8(+) T cells but not CD4(+) T cells.

AB - The main DC subsets in murine spleen arise from BM-derived precursors. Recently, a novel APC type was described in spleen. To determine if spleen contains the progenitors of this subset, a stromal coculture system was used to assess candidate progenitors for their hematopoietic potential. Here, the progenitor of that subset is identified as a spleen endogenous Lin(-)c-kit(lo) hematopoietic progenitor and is most highly enriched among the Lin(-)c-kit(lo)CD34(+) subset. Dendritic-like cells produced in vitro functionally resemble the previously described in vivo equivalent subset with high endocytic capacity and capability for antigen-specific activation of CD8(+) T cells but not CD4(+) T cells.

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DO - 10.1189/jlb.0512260

M3 - Article

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EP - 69

JO - Journal of Leukocyte Biology

JF - Journal of Leukocyte Biology

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