Nerve-evoked and phasic contractions of the rat bladder: Effects of low testosterone and treatment with the selective and rogen receptor modulator trenbolone

Donna J Sellers, Roselyn Rose'meyer, Joss Du Toit, D. Donner

Research output: Contribution to conferenceAbstractResearchpeer-review

Abstract

Overactive bladder is prevalent with aging and recent evidence suggests androgen deficiency may playa role (Koritsiadis et al., 2008). Androgen receptors are expressed throughout the bladder (Chalvamane et al., 2010),although the role of testosterone in bladder function remains unclear.Aims. To investigate the effect of low testosterone and treatment with a selective androgen receptor modulator oncontractility of rat bladder strips.Methods. Wistar rats (8 weeks) were orchiectomised (5% isoflurane). 8 weeks later, half received trenboloneacetate (2mg/kg/day for 8 weeks, sc.). Sham-operated controls received vehicle. Isolated bladder strips weremounted in tissue baths (Krebs-bicarbonate solution, 1.5g tension, 37°C). Amplitude and frequency of phasiccontractions (PCs) and nerve-evoked contractions (EFS) (1-50Hz, 0.01ms duration, 40V, 5s every 100s) wereexamined.Results. Orchiectomised rats had low serum testosterone vs controls and trenbolone-treated (0.24±0.05 vs 1.68±0.18vs 0.21±0.04ng/ml, P<0.001). Amplitude of PCs was increased in orchiectomised rat bladder strips (0.0266±0.0025vs 0.0016±0.0027g/mg, P<0.05), whilst frequency was reduced (32±5 vs 50±5 events/5mins, P<0.05). Trenbolonetreatmentprevented the increased amplitude, but not the decreased frequency. EFS contractions were depressed inorchiectomised bladder strips and α,β-methylene-ATP (10μM) produced greater inhibition vs controls (80.3±2.8%vs 6.6±3.8%, P<0.01). Trenbolone-treatment did not prevent depressed EFS contractions, but did prevent theincreased purinergic component. Atropine (1μM) plus α,β-methylene-ATP completely abolished EFS responses inorchiectomised bladder strips, but not in control and trenbolone-treated, where the remaining response wasunaffected by L-NNA (100μM).Discussion. Orchiectomy causes increased phasic contractions of rat bladder strips and depressed nerve-evokedcontractions, in which ATP plays a greater role, supporting a role for testosterone in normal bladder function.Trenbolone prevented only some of these alterations, suggesting the actions of testosterone may be partly mediatedvia conversion to other sex steroids.
Original languageEnglish
Pages207-207
Number of pages1
Publication statusPublished - 2012
EventJoint ASCEPT- APSA 2012 Conference : Medication Safety - Sydney Convention and Exhibition Centre, Sydney, Australia
Duration: 2 Dec 20125 Dec 2012
http://www.ascept-apsa.com/

Conference

ConferenceJoint ASCEPT- APSA 2012 Conference
CountryAustralia
CitySydney
Period2/12/125/12/12
Internet address

Fingerprint

Trenbolone Acetate
Testosterone
Urinary Bladder
Therapeutics
Adenosine Triphosphate
Androgen Receptors
Overactive Urinary Bladder
Orchiectomy
Isoflurane
Bicarbonates
Baths
Atropine
Androgens
Wistar Rats
Steroids

Cite this

Sellers, D. J., Rose'meyer, R., Du Toit, J., & Donner, D. (2012). Nerve-evoked and phasic contractions of the rat bladder: Effects of low testosterone and treatment with the selective and rogen receptor modulator trenbolone. 207-207. Abstract from Joint ASCEPT- APSA 2012 Conference , Sydney, Australia.
Sellers, Donna J ; Rose'meyer, Roselyn ; Du Toit, Joss ; Donner, D. / Nerve-evoked and phasic contractions of the rat bladder : Effects of low testosterone and treatment with the selective and rogen receptor modulator trenbolone. Abstract from Joint ASCEPT- APSA 2012 Conference , Sydney, Australia.1 p.
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abstract = "Overactive bladder is prevalent with aging and recent evidence suggests androgen deficiency may playa role (Koritsiadis et al., 2008). Androgen receptors are expressed throughout the bladder (Chalvamane et al., 2010),although the role of testosterone in bladder function remains unclear.Aims. To investigate the effect of low testosterone and treatment with a selective androgen receptor modulator oncontractility of rat bladder strips.Methods. Wistar rats (8 weeks) were orchiectomised (5{\%} isoflurane). 8 weeks later, half received trenboloneacetate (2mg/kg/day for 8 weeks, sc.). Sham-operated controls received vehicle. Isolated bladder strips weremounted in tissue baths (Krebs-bicarbonate solution, 1.5g tension, 37°C). Amplitude and frequency of phasiccontractions (PCs) and nerve-evoked contractions (EFS) (1-50Hz, 0.01ms duration, 40V, 5s every 100s) wereexamined.Results. Orchiectomised rats had low serum testosterone vs controls and trenbolone-treated (0.24±0.05 vs 1.68±0.18vs 0.21±0.04ng/ml, P<0.001). Amplitude of PCs was increased in orchiectomised rat bladder strips (0.0266±0.0025vs 0.0016±0.0027g/mg, P<0.05), whilst frequency was reduced (32±5 vs 50±5 events/5mins, P<0.05). Trenbolonetreatmentprevented the increased amplitude, but not the decreased frequency. EFS contractions were depressed inorchiectomised bladder strips and α,β-methylene-ATP (10μM) produced greater inhibition vs controls (80.3±2.8{\%}vs 6.6±3.8{\%}, P<0.01). Trenbolone-treatment did not prevent depressed EFS contractions, but did prevent theincreased purinergic component. Atropine (1μM) plus α,β-methylene-ATP completely abolished EFS responses inorchiectomised bladder strips, but not in control and trenbolone-treated, where the remaining response wasunaffected by L-NNA (100μM).Discussion. Orchiectomy causes increased phasic contractions of rat bladder strips and depressed nerve-evokedcontractions, in which ATP plays a greater role, supporting a role for testosterone in normal bladder function.Trenbolone prevented only some of these alterations, suggesting the actions of testosterone may be partly mediatedvia conversion to other sex steroids.",
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Sellers, DJ, Rose'meyer, R, Du Toit, J & Donner, D 2012, 'Nerve-evoked and phasic contractions of the rat bladder: Effects of low testosterone and treatment with the selective and rogen receptor modulator trenbolone' Joint ASCEPT- APSA 2012 Conference , Sydney, Australia, 2/12/12 - 5/12/12, pp. 207-207.

Nerve-evoked and phasic contractions of the rat bladder : Effects of low testosterone and treatment with the selective and rogen receptor modulator trenbolone. / Sellers, Donna J; Rose'meyer, Roselyn; Du Toit, Joss; Donner, D.

2012. 207-207 Abstract from Joint ASCEPT- APSA 2012 Conference , Sydney, Australia.

Research output: Contribution to conferenceAbstractResearchpeer-review

TY - CONF

T1 - Nerve-evoked and phasic contractions of the rat bladder

T2 - Effects of low testosterone and treatment with the selective and rogen receptor modulator trenbolone

AU - Sellers, Donna J

AU - Rose'meyer, Roselyn

AU - Du Toit, Joss

AU - Donner, D.

PY - 2012

Y1 - 2012

N2 - Overactive bladder is prevalent with aging and recent evidence suggests androgen deficiency may playa role (Koritsiadis et al., 2008). Androgen receptors are expressed throughout the bladder (Chalvamane et al., 2010),although the role of testosterone in bladder function remains unclear.Aims. To investigate the effect of low testosterone and treatment with a selective androgen receptor modulator oncontractility of rat bladder strips.Methods. Wistar rats (8 weeks) were orchiectomised (5% isoflurane). 8 weeks later, half received trenboloneacetate (2mg/kg/day for 8 weeks, sc.). Sham-operated controls received vehicle. Isolated bladder strips weremounted in tissue baths (Krebs-bicarbonate solution, 1.5g tension, 37°C). Amplitude and frequency of phasiccontractions (PCs) and nerve-evoked contractions (EFS) (1-50Hz, 0.01ms duration, 40V, 5s every 100s) wereexamined.Results. Orchiectomised rats had low serum testosterone vs controls and trenbolone-treated (0.24±0.05 vs 1.68±0.18vs 0.21±0.04ng/ml, P<0.001). Amplitude of PCs was increased in orchiectomised rat bladder strips (0.0266±0.0025vs 0.0016±0.0027g/mg, P<0.05), whilst frequency was reduced (32±5 vs 50±5 events/5mins, P<0.05). Trenbolonetreatmentprevented the increased amplitude, but not the decreased frequency. EFS contractions were depressed inorchiectomised bladder strips and α,β-methylene-ATP (10μM) produced greater inhibition vs controls (80.3±2.8%vs 6.6±3.8%, P<0.01). Trenbolone-treatment did not prevent depressed EFS contractions, but did prevent theincreased purinergic component. Atropine (1μM) plus α,β-methylene-ATP completely abolished EFS responses inorchiectomised bladder strips, but not in control and trenbolone-treated, where the remaining response wasunaffected by L-NNA (100μM).Discussion. Orchiectomy causes increased phasic contractions of rat bladder strips and depressed nerve-evokedcontractions, in which ATP plays a greater role, supporting a role for testosterone in normal bladder function.Trenbolone prevented only some of these alterations, suggesting the actions of testosterone may be partly mediatedvia conversion to other sex steroids.

AB - Overactive bladder is prevalent with aging and recent evidence suggests androgen deficiency may playa role (Koritsiadis et al., 2008). Androgen receptors are expressed throughout the bladder (Chalvamane et al., 2010),although the role of testosterone in bladder function remains unclear.Aims. To investigate the effect of low testosterone and treatment with a selective androgen receptor modulator oncontractility of rat bladder strips.Methods. Wistar rats (8 weeks) were orchiectomised (5% isoflurane). 8 weeks later, half received trenboloneacetate (2mg/kg/day for 8 weeks, sc.). Sham-operated controls received vehicle. Isolated bladder strips weremounted in tissue baths (Krebs-bicarbonate solution, 1.5g tension, 37°C). Amplitude and frequency of phasiccontractions (PCs) and nerve-evoked contractions (EFS) (1-50Hz, 0.01ms duration, 40V, 5s every 100s) wereexamined.Results. Orchiectomised rats had low serum testosterone vs controls and trenbolone-treated (0.24±0.05 vs 1.68±0.18vs 0.21±0.04ng/ml, P<0.001). Amplitude of PCs was increased in orchiectomised rat bladder strips (0.0266±0.0025vs 0.0016±0.0027g/mg, P<0.05), whilst frequency was reduced (32±5 vs 50±5 events/5mins, P<0.05). Trenbolonetreatmentprevented the increased amplitude, but not the decreased frequency. EFS contractions were depressed inorchiectomised bladder strips and α,β-methylene-ATP (10μM) produced greater inhibition vs controls (80.3±2.8%vs 6.6±3.8%, P<0.01). Trenbolone-treatment did not prevent depressed EFS contractions, but did prevent theincreased purinergic component. Atropine (1μM) plus α,β-methylene-ATP completely abolished EFS responses inorchiectomised bladder strips, but not in control and trenbolone-treated, where the remaining response wasunaffected by L-NNA (100μM).Discussion. Orchiectomy causes increased phasic contractions of rat bladder strips and depressed nerve-evokedcontractions, in which ATP plays a greater role, supporting a role for testosterone in normal bladder function.Trenbolone prevented only some of these alterations, suggesting the actions of testosterone may be partly mediatedvia conversion to other sex steroids.

M3 - Abstract

SP - 207

EP - 207

ER -

Sellers DJ, Rose'meyer R, Du Toit J, Donner D. Nerve-evoked and phasic contractions of the rat bladder: Effects of low testosterone and treatment with the selective and rogen receptor modulator trenbolone. 2012. Abstract from Joint ASCEPT- APSA 2012 Conference , Sydney, Australia.