Natural anti-self reactivity which maps to MHC class I genes

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Responder spleen cells of [B10.A(5R) x B10.A(2R)]F1 hybrid mice cultured in vitro with irradiated parental spleen cells, generate a cytotoxic T cell response which maps to the H-2Dd and H-2(K-I)b genes of the B10.A(5R) parent. Specific reactivity of (5R x 2R) cells to B10.A(5R) parental (P) stimulators and not to F1 hybrid or B10.A(2R) stimulators was also demonstrated as an F1 anti-P proliferative response. Generation of a (5R x 2R) anti-5R response has also been noted during 2 degrees in vitro anti-viral cytotoxic responses where (5R x 2R) cells from either ectromelia or Sendai virus primed mice have been stimulated in vitro with infected 5R cells, but not when stimulated with infected 2R or (5R x 2R) cells. The generation of these responses is dependent on the use of parental stimulators for F1 hybrid cells during in vitro culture. All data is consistent with the hypothesis that (5R x 2R) F1 cells are not tolerant of 5R cells, and of the 4-fold higher level of Class I H-2Kb/Dd antigens which they express.

Original languageEnglish
Pages (from-to)438-449
Number of pages12
JournalImmunobiology
Volume181
Issue number4-5
DOIs
Publication statusPublished - Nov 1990
Externally publishedYes

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MHC Class I Genes
Ectromelia virus
Spleen
Sendai virus
Hybrid Cells
T-Lymphocytes
Antigens
In Vitro Techniques

Cite this

@article{28960e039e914562b785129c870102f6,
title = "Natural anti-self reactivity which maps to MHC class I genes",
abstract = "Responder spleen cells of [B10.A(5R) x B10.A(2R)]F1 hybrid mice cultured in vitro with irradiated parental spleen cells, generate a cytotoxic T cell response which maps to the H-2Dd and H-2(K-I)b genes of the B10.A(5R) parent. Specific reactivity of (5R x 2R) cells to B10.A(5R) parental (P) stimulators and not to F1 hybrid or B10.A(2R) stimulators was also demonstrated as an F1 anti-P proliferative response. Generation of a (5R x 2R) anti-5R response has also been noted during 2 degrees in vitro anti-viral cytotoxic responses where (5R x 2R) cells from either ectromelia or Sendai virus primed mice have been stimulated in vitro with infected 5R cells, but not when stimulated with infected 2R or (5R x 2R) cells. The generation of these responses is dependent on the use of parental stimulators for F1 hybrid cells during in vitro culture. All data is consistent with the hypothesis that (5R x 2R) F1 cells are not tolerant of 5R cells, and of the 4-fold higher level of Class I H-2Kb/Dd antigens which they express.",
author = "O'Neill, {H C}",
year = "1990",
month = "11",
doi = "10.1016/S0171-2985(11)80512-7",
language = "English",
volume = "181",
pages = "438--449",
journal = "Zeitschrift fur Immunitatsforschung",
issn = "0171-2985",
publisher = "Urban und Fischer Verlag GmbH und Co. KG",
number = "4-5",

}

Natural anti-self reactivity which maps to MHC class I genes. / O'Neill, H C.

In: Immunobiology, Vol. 181, No. 4-5, 11.1990, p. 438-449.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

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AU - O'Neill, H C

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N2 - Responder spleen cells of [B10.A(5R) x B10.A(2R)]F1 hybrid mice cultured in vitro with irradiated parental spleen cells, generate a cytotoxic T cell response which maps to the H-2Dd and H-2(K-I)b genes of the B10.A(5R) parent. Specific reactivity of (5R x 2R) cells to B10.A(5R) parental (P) stimulators and not to F1 hybrid or B10.A(2R) stimulators was also demonstrated as an F1 anti-P proliferative response. Generation of a (5R x 2R) anti-5R response has also been noted during 2 degrees in vitro anti-viral cytotoxic responses where (5R x 2R) cells from either ectromelia or Sendai virus primed mice have been stimulated in vitro with infected 5R cells, but not when stimulated with infected 2R or (5R x 2R) cells. The generation of these responses is dependent on the use of parental stimulators for F1 hybrid cells during in vitro culture. All data is consistent with the hypothesis that (5R x 2R) F1 cells are not tolerant of 5R cells, and of the 4-fold higher level of Class I H-2Kb/Dd antigens which they express.

AB - Responder spleen cells of [B10.A(5R) x B10.A(2R)]F1 hybrid mice cultured in vitro with irradiated parental spleen cells, generate a cytotoxic T cell response which maps to the H-2Dd and H-2(K-I)b genes of the B10.A(5R) parent. Specific reactivity of (5R x 2R) cells to B10.A(5R) parental (P) stimulators and not to F1 hybrid or B10.A(2R) stimulators was also demonstrated as an F1 anti-P proliferative response. Generation of a (5R x 2R) anti-5R response has also been noted during 2 degrees in vitro anti-viral cytotoxic responses where (5R x 2R) cells from either ectromelia or Sendai virus primed mice have been stimulated in vitro with infected 5R cells, but not when stimulated with infected 2R or (5R x 2R) cells. The generation of these responses is dependent on the use of parental stimulators for F1 hybrid cells during in vitro culture. All data is consistent with the hypothesis that (5R x 2R) F1 cells are not tolerant of 5R cells, and of the 4-fold higher level of Class I H-2Kb/Dd antigens which they express.

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