Nanospiderwebs: Artificial 3D Extracellular Matrix from Nanofibers by Novel Clinical Grade Electrospinning for Stem Cell Delivery

Mohammad A. Alamein, Qin Liu, Sebastien Stephens, Stuart Skabo, Frauke Warnke, Robert Bourke, Peter Heiner, Patrick H. Warnke

Research output: Contribution to journalArticleResearchpeer-review

25 Citations (Scopus)

Abstract

Novel clinical grade electrospinning methods could provide three-dimensional (3D) nanostructured biomaterials comprising of synthetic or natural biopolymer nanofibers. Such advanced materials could potentially mimic the natural extracellular matrix (ECM) accurately and may provide superior niche-like spaces on the subcellular scale for optimal stem-cell attachment and individual cell homing in regenerative therapies. The goal of this study was to design several novel "nanofibrous extracellular matrices" (NF-ECMs) with a natural mesh-like 3D architecture through a unique needle-free multi-jet electrospinning method in highly controlled manner to comply with good manufacturing practices (GMP) for the production of advanced healthcare materials for regenerative medicine, and to test cellular behavior of human mesenchymal stem cells (HMSCs) on these. Biopolymers manufactured as 3D NF-ECM meshes under clinical grade GMP-like conditions show higher intrinsic cytobiocompatibility with superior cell integration and proliferation if compared to their 2D counterparts or a clinically-approved collagen membrane.

Original languageEnglish
Pages (from-to)702-717
Number of pages16
JournalAdvanced Healthcare Materials
Volume2
Issue number5
DOIs
Publication statusPublished - May 2013

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Nanofibers
Electrospinning
Stem cells
Extracellular Matrix
Biopolymers
Stem Cells
Intracellular Space
Regenerative Medicine
Biocompatible Materials
Mesenchymal Stromal Cells
Collagen
Biomaterials
Needles
Cell Proliferation
Membranes
Delivery of Health Care
Therapeutics

Cite this

Alamein, Mohammad A. ; Liu, Qin ; Stephens, Sebastien ; Skabo, Stuart ; Warnke, Frauke ; Bourke, Robert ; Heiner, Peter ; Warnke, Patrick H. / Nanospiderwebs : Artificial 3D Extracellular Matrix from Nanofibers by Novel Clinical Grade Electrospinning for Stem Cell Delivery. In: Advanced Healthcare Materials. 2013 ; Vol. 2, No. 5. pp. 702-717.
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Nanospiderwebs : Artificial 3D Extracellular Matrix from Nanofibers by Novel Clinical Grade Electrospinning for Stem Cell Delivery. / Alamein, Mohammad A.; Liu, Qin; Stephens, Sebastien; Skabo, Stuart; Warnke, Frauke; Bourke, Robert; Heiner, Peter; Warnke, Patrick H.

In: Advanced Healthcare Materials, Vol. 2, No. 5, 05.2013, p. 702-717.

Research output: Contribution to journalArticleResearchpeer-review

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