NADPH diaphorase localization and nitric oxide synthetase activity in the retina and anterior uvea of the rabbit eye

N. N. Osborne, N. L. Barnett, A. J. Herrera

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Abstract

The distribution of the enzyme nicotinamide adenine dinucleotide phosphate (NADPH) diaphorase was examined histochemically in the retina, iris, ciliary processes, cornea and conjunctiva of the rabbit eye. The epithelial cells of the ciliary process, iris, conjunctiva and, to a lesser extent, the cornea all showed intense staining. In the retina, staining for NADPH diaphorase was intense in the inner segments of the photoreceptors and a sparsely distributed population of amacrine cells. In addition, another population of amacrine cells, some presumed ganglion cells as well as a number of horizontal cells, stained less intensely for the enzyme. The retina, ciliary processes and, as a comparison, the cerebellum of the rabbit all contain nitric oxide synthetase (NOS) activity, as each tissue can metabolize citrulline from arginine. This process is Ca2+ dependent and is reduced by the NOS inhibitor,NG-monomethyl-l-arginine. The presence of NOS activity in the ciliary processes and the localization of NADPH diaphorase in the ciliary epithelial cells are of significance as they suggest that the ciliary epithelial cells may contain NOS which would imply a role for nitric oxide in aqueous humour production.

Original languageEnglish
Pages (from-to)194-198
Number of pages5
JournalBrain Research
Volume610
Issue number2
DOIs
Publication statusPublished - 7 May 1993
Externally publishedYes

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Uvea
NADP
Nitric Oxide Synthase
Retina
Rabbits
Amacrine Cells
Epithelial Cells
Conjunctiva
Iris
Cornea
Arginine
Staining and Labeling
Citrulline
Aqueous Humor
Enzymes
Ganglia
Cerebellum
Population
Nitric Oxide
Cell Count

Cite this

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abstract = "The distribution of the enzyme nicotinamide adenine dinucleotide phosphate (NADPH) diaphorase was examined histochemically in the retina, iris, ciliary processes, cornea and conjunctiva of the rabbit eye. The epithelial cells of the ciliary process, iris, conjunctiva and, to a lesser extent, the cornea all showed intense staining. In the retina, staining for NADPH diaphorase was intense in the inner segments of the photoreceptors and a sparsely distributed population of amacrine cells. In addition, another population of amacrine cells, some presumed ganglion cells as well as a number of horizontal cells, stained less intensely for the enzyme. The retina, ciliary processes and, as a comparison, the cerebellum of the rabbit all contain nitric oxide synthetase (NOS) activity, as each tissue can metabolize citrulline from arginine. This process is Ca2+ dependent and is reduced by the NOS inhibitor,NG-monomethyl-l-arginine. The presence of NOS activity in the ciliary processes and the localization of NADPH diaphorase in the ciliary epithelial cells are of significance as they suggest that the ciliary epithelial cells may contain NOS which would imply a role for nitric oxide in aqueous humour production.",
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NADPH diaphorase localization and nitric oxide synthetase activity in the retina and anterior uvea of the rabbit eye. / Osborne, N. N.; Barnett, N. L.; Herrera, A. J.

In: Brain Research, Vol. 610, No. 2, 07.05.1993, p. 194-198.

Research output: Contribution to journalArticleResearchpeer-review

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